(Commercial Posting) New Monitoring slides for fluorescence based systems

[Gautier Papon]

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Many members of the confocal list are also clients or people we meet in conference. Just a short message to introduce our new products : http://argolight.com/new-generation-argolight/

Thanks
Gautier Papon, CEO
 

[Zdenek Svindrych-2]

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Dear Gautier,
is there any detailed information available concerning the lifetime of the
luminescecne of your patterns?


I quickly went through some of the papers of L. Canioni's group at the
university of Bordeax about direct laser writing into Ag-dopes zinc-
phosphate glasses, some of them show lifetime histograms, but the decays
differ between papers and they may not be relevant for your 'tailored' glass
composition.

I know it's difficult to characterize, as the lifetime will most likely
depend on both the excitation and the emission wavelength, and some lifetime
components may stretch to microsecond or more...

But I'm mostly interested in stability of lifetimes (as well as intensity)
with time and temperature. Also, can something analogous to absorption cross
-section and quantum yield be characterized for your slides? And can you
expand more on water (PBS, glycerol) compatibility of the slides?

Thank you.

Best, zdenek


--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
University of Virginia, Charlottesville, VA
http://www.kcci.virginia.edu/
tel: [phone]
Annual FRET Workshop: http://kcci.virginia.edu/workshop-2017


---------- Původní zpráva ----------
Od: Gautier Papon <[hidden email]>
Komu: [hidden email]
Datum: 14. 6. 2016 8:43:17
Předmět: (Commercial Posting) New Monitoring slides for fluorescence based
systems

"*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

Many members of the confocal list are also clients or people we meet in
conference. Just a short message to introduce our new products : http://
argolight.com/new-generation-argolight/

Thanks
Gautier Papon, CEO"

[George McNamara]

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Hi zdenek,

FLIM dye standards ... Ben Barbieri, ISS, showed Rose Bengal in several
solvents, during his vendor talk today at the Automated Microscopy
conference here in Houston,
http://www.txsact.org/events/application-of-automated-microscopy-and-image-informatics-to-cancer-research

I did not see many Rose Bengal entries on ISS's several online tables of
FLIM values for many well behaved dyes at
http://www.iss.com/resources/reference/data_tables/index.html

so if you need those, I suggest contacting ISS though their web site.

Sincerely,

George



On 6/14/2016 10:20 AM, [hidden email] wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Gautier,
> is there any detailed information available concerning the lifetime of the
> luminescecne of your patterns?
>
>
> I quickly went through some of the papers of L. Canioni's group at the
> university of Bordeax about direct laser writing into Ag-dopes zinc-
> phosphate glasses, some of them show lifetime histograms, but the decays
> differ between papers and they may not be relevant for your 'tailored' glass
> composition.
>
> I know it's difficult to characterize, as the lifetime will most likely
> depend on both the excitation and the emission wavelength, and some lifetime
> components may stretch to microsecond or more...
>
> But I'm mostly interested in stability of lifetimes (as well as intensity)
> with time and temperature. Also, can something analogous to absorption cross
> -section and quantum yield be characterized for your slides? And can you
> expand more on water (PBS, glycerol) compatibility of the slides?
>
> Thank you.
>
> Best, zdenek
>
>

--



George McNamara, Ph.D.
Houston, TX 77054
[hidden email]
713-239-0365 home
305-764-2081 cell
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/   Tattletales and T-Bow

[Gautier Papon]

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*****

Dear Zdenek,

Our glass is indeed different from the ones used in these publications - That being said we performed some lifetime measurement using FLIM : two main decay components of (0.25 ± 0.05) ns and (2.50 ± 0.50) ns were been measured. The measurements were made with a 400 nm excitation collected at 500 nm (+/- 20nm). This is the only lifetime measurement we performed.

The size of our feature (<120 nm) makes the measurement of quantum yield of our emitter were difficult, so far our attempt to measure a value were not conclusive.

Concerning the compatibility of the slide, they are compatible with immersion oil, water and of course dry objectives : For widefield systems, there is no issue to use water as an immersion medium. To give you an idea, we irradiated one of our Argo-HM slides with the 365 nm LED of our Colibri 2, at 100% of illumination power, using the DAPI filter cube and a 40x/1.2 water immersion objective. After one hour of continuous exposure, nothing happened. After six hours of continuous exposure, a slightly whitish layer appeared at the surface, making the glass a little less transparent.

So if you expose the Argo-HM slide to light with water as an immersion medium, there is no issue as long as the exposure time remains within a few minutes, which I think is sufficient to acquire images of the patterns. We chose to give a conservative value of 5 minutes in our user guide to prevent any excessive exposition. For confocal systems, where light power densities can be 3 orders of magnitude more important than for widefield systems, the conservative value of 5 minutes has to be strictly respected.
 

Gautier Papon, Ph.D
------------------------------------
Tel : [phone]
Argolight, A Precision Company
www.argolight.com 
——————————
Argolight is Carl Zeiss Choice for microscope quality assessment and monitoring – Read About it on www.argolight.com  

-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de [hidden email]
Envoyé : mardi 14 juin 2016 17:21
À : [hidden email]
Objet : Re: (Commercial Posting) New Monitoring slides for fluorescence based systems

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Gautier,
is there any detailed information available concerning the lifetime of the luminescecne of your patterns?


I quickly went through some of the papers of L. Canioni's group at the
university of Bordeax about direct laser writing into Ag-dopes zinc-
phosphate glasses, some of them show lifetime histograms, but the decays
differ between papers and they may not be relevant for your 'tailored' glass
composition.

I know it's difficult to characterize, as the lifetime will most likely
depend on both the excitation and the emission wavelength, and some lifetime
components may stretch to microsecond or more...

But I'm mostly interested in stability of lifetimes (as well as intensity)
with time and temperature. Also, can something analogous to absorption cross
-section and quantum yield be characterized for your slides? And can you
expand more on water (PBS, glycerol) compatibility of the slides?

Thank you.

Best, zdenek


--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
University of Virginia, Charlottesville, VA
http://www.kcci.virginia.edu/
tel: [phone]
Annual FRET Workshop: http://kcci.virginia.edu/workshop-2017


---------- Původní zpráva ----------
Od: Gautier Papon <[hidden email]>
Komu: [hidden email]
Datum: 14. 6. 2016 8:43:17
Předmět: (Commercial Posting) New Monitoring slides for fluorescence based
systems

"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Many members of the confocal list are also clients or people we meet in
conference. Just a short message to introduce our new products : http://
argolight.com/new-generation-argolight/

Thanks
Gautier Papon, CEO"

[Sylvie Le Guyader]

*****
To join, leave or search the confocal microscopy listserv, go to:
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Hi Gauthier

Which immersion oil did you try? As discussed before on this forum the oils sold by Zeiss and by Nikon do not have the same chemistry at all. The standard Nikon oil dissolves Ibidi polymer for example whereas the Zeiss oil doesn’t.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7,
Novum, G lift, floor 6
14157 Huddinge
Sweden
mobile: [phone]
office: [phone] New number!
LCI website


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gautier Papon
Sent: den 16 juni 2016 14:53
To: [hidden email]
Subject: Re: (Commercial Posting) New Monitoring slides for fluorescence based systems

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Zdenek,

Our glass is indeed different from the ones used in these publications - That being said we performed some lifetime measurement using FLIM : two main decay components of (0.25 ± 0.05) ns and (2.50 ± 0.50) ns were been measured. The measurements were made with a 400 nm excitation collected at 500 nm (+/- 20nm). This is the only lifetime measurement we performed.

The size of our feature (<120 nm) makes the measurement of quantum yield of our emitter were difficult, so far our attempt to measure a value were not conclusive.

Concerning the compatibility of the slide, they are compatible with immersion oil, water and of course dry objectives : For widefield systems, there is no issue to use water as an immersion medium. To give you an idea, we irradiated one of our Argo-HM slides with the 365 nm LED of our Colibri 2, at 100% of illumination power, using the DAPI filter cube and a 40x/1.2 water immersion objective. After one hour of continuous exposure, nothing happened. After six hours of continuous exposure, a slightly whitish layer appeared at the surface, making the glass a little less transparent.

So if you expose the Argo-HM slide to light with water as an immersion medium, there is no issue as long as the exposure time remains within a few minutes, which I think is sufficient to acquire images of the patterns. We chose to give a conservative value of 5 minutes in our user guide to prevent any excessive exposition. For confocal systems, where light power densities can be 3 orders of magnitude more important than for widefield systems, the conservative value of 5 minutes has to be strictly respected.
 

Gautier Papon, Ph.D
------------------------------------
Tel : [phone]
Argolight, A Precision Company
www.argolight.com
——————————
Argolight is Carl Zeiss Choice for microscope quality assessment and monitoring – Read About it on www.argolight.com  

-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de [hidden email] Envoyé : mardi 14 juin 2016 17:21 À : [hidden email] Objet : Re: (Commercial Posting) New Monitoring slides for fluorescence based systems

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Gautier,
is there any detailed information available concerning the lifetime of the luminescecne of your patterns?


I quickly went through some of the papers of L. Canioni's group at the university of Bordeax about direct laser writing into Ag-dopes zinc- phosphate glasses, some of them show lifetime histograms, but the decays differ between papers and they may not be relevant for your 'tailored' glass composition.

I know it's difficult to characterize, as the lifetime will most likely depend on both the excitation and the emission wavelength, and some lifetime components may stretch to microsecond or more...

But I'm mostly interested in stability of lifetimes (as well as intensity) with time and temperature. Also, can something analogous to absorption cross -section and quantum yield be characterized for your slides? And can you expand more on water (PBS, glycerol) compatibility of the slides?

Thank you.

Best, zdenek


--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003) University of Virginia, Charlottesville, VA http://www.kcci.virginia.edu/
tel: [phone]
Annual FRET Workshop: http://kcci.virginia.edu/workshop-2017


---------- Původní zpráva ----------
Od: Gautier Papon <[hidden email]>
Komu: [hidden email]
Datum: 14. 6. 2016 8:43:17
Předmět: (Commercial Posting) New Monitoring slides for fluorescence based systems

"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Many members of the confocal list are also clients or people we meet in conference. Just a short message to introduce our new products : http:// argolight.com/new-generation-argolight/

Thanks
Gautier Papon, CEO"

[Gautier Papon]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Sylvie,

So far our slide have been used with all brands of oil by our clients and ourselves and there had been no issues. The slide were also tested by all four major manufacturers (Leica, Zeiss, Nikon, Olympus) in their lab and, and oil incompatibly was not found. Our material is a glass and not a polymer, while certain type of glass can be instable, ours was designed and tweaked to have the best stability over time.

Best regards,
Gautier


Gautier Papon, Ph.D
------------------------------------
Tel : [phone]
Argolight, A Precision Company
www.argolight.com 
——————————
Argolight is Carl Zeiss Choice for microscope quality assessment and monitoring – Read About it on www.argolight.com  

-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de Sylvie Le Guyader
Envoyé : jeudi 16 juin 2016 15:15
À : [hidden email]
Objet : Re: (Commercial Posting) New Monitoring slides for fluorescence based systems

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Gauthier

Which immersion oil did you try? As discussed before on this forum the oils sold by Zeiss and by Nikon do not have the same chemistry at all. The standard Nikon oil dissolves Ibidi polymer for example whereas the Zeiss oil doesn’t.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7,
Novum, G lift, floor 6
14157 Huddinge
Sweden
mobile: [phone]
office: [phone] New number!
LCI website


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gautier Papon
Sent: den 16 juni 2016 14:53
To: [hidden email]
Subject: Re: (Commercial Posting) New Monitoring slides for fluorescence based systems

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Zdenek,

Our glass is indeed different from the ones used in these publications - That being said we performed some lifetime measurement using FLIM : two main decay components of (0.25 ± 0.05) ns and (2.50 ± 0.50) ns were been measured. The measurements were made with a 400 nm excitation collected at 500 nm (+/- 20nm). This is the only lifetime measurement we performed.

The size of our feature (<120 nm) makes the measurement of quantum yield of our emitter were difficult, so far our attempt to measure a value were not conclusive.

Concerning the compatibility of the slide, they are compatible with immersion oil, water and of course dry objectives : For widefield systems, there is no issue to use water as an immersion medium. To give you an idea, we irradiated one of our Argo-HM slides with the 365 nm LED of our Colibri 2, at 100% of illumination power, using the DAPI filter cube and a 40x/1.2 water immersion objective. After one hour of continuous exposure, nothing happened. After six hours of continuous exposure, a slightly whitish layer appeared at the surface, making the glass a little less transparent.

So if you expose the Argo-HM slide to light with water as an immersion medium, there is no issue as long as the exposure time remains within a few minutes, which I think is sufficient to acquire images of the patterns. We chose to give a conservative value of 5 minutes in our user guide to prevent any excessive exposition. For confocal systems, where light power densities can be 3 orders of magnitude more important than for widefield systems, the conservative value of 5 minutes has to be strictly respected.
 

Gautier Papon, Ph.D
------------------------------------
Tel : [phone]
Argolight, A Precision Company
www.argolight.com
——————————
Argolight is Carl Zeiss Choice for microscope quality assessment and monitoring – Read About it on www.argolight.com  

-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de [hidden email] Envoyé : mardi 14 juin 2016 17:21 À : [hidden email] Objet : Re: (Commercial Posting) New Monitoring slides for fluorescence based systems

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Gautier,
is there any detailed information available concerning the lifetime of the luminescecne of your patterns?


I quickly went through some of the papers of L. Canioni's group at the university of Bordeax about direct laser writing into Ag-dopes zinc- phosphate glasses, some of them show lifetime histograms, but the decays differ between papers and they may not be relevant for your 'tailored' glass composition.

I know it's difficult to characterize, as the lifetime will most likely depend on both the excitation and the emission wavelength, and some lifetime components may stretch to microsecond or more...

But I'm mostly interested in stability of lifetimes (as well as intensity) with time and temperature. Also, can something analogous to absorption cross -section and quantum yield be characterized for your slides? And can you expand more on water (PBS, glycerol) compatibility of the slides?

Thank you.

Best, zdenek


--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003) University of Virginia, Charlottesville, VA http://www.kcci.virginia.edu/
tel: [phone]
Annual FRET Workshop: http://kcci.virginia.edu/workshop-2017


---------- Původní zpráva ----------
Od: Gautier Papon <[hidden email]>
Komu: [hidden email]
Datum: 14. 6. 2016 8:43:17
Předmět: (Commercial Posting) New Monitoring slides for fluorescence based systems

"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Many members of the confocal list are also clients or people we meet in conference. Just a short message to introduce our new products : http:// argolight.com/new-generation-argolight/

Thanks
Gautier Papon, CEO"