Commercial laser tweezer systems

[Csúcs Gábor-3]

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Dear All,

For a project we are looking into the different possibilities to equip an existing microscope (Nikon Ti-Eclipse) with an optical tweezer system. Our primary application (right now) would be single cell manipulation (moving them) but an existing future possibility for an (easy) upgrade for a force measurement system would be also appreciated. Does anyone have experience with commercial systems? During my search I have found solutions from Thorlabs, JPK, Elliot scientific, Aresis, MMI and Zeiss (though for a Nikon scope this is probably not an option). Is there any other source existing? ). It would be also nice if the tweezer could be used in combination with Micromanager (used for imaging). Some posts smention also Arry but this company seem to disappeared. Thanks for any feedback!

Greetings       Gabor

[Philip Nicovich]

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Gabor,

We have a JPK system built on top of a Nikon Ti-E.  The system does
everything it's supposed to do - two traps, one controllable with the
mouse, plus lots of options for force measurements. As complicated as an
optical tweezers system can be, this one is actually reasonably easy to
use. The construction and design is v. high quality, with lots of built-in
alignment and calibration features that work well.  We have had it for
nearly 4 years now and have had zero issues on the JPK side.  Even lifted
the whole system onto dolleys, pushed it into the freight elevator and
moved it up 4 floors, did a couple of the calibration procedures, and had
it back to original spec in an easy afternoon.

Initial focusing can be a bit of a bear and at least on our system new a
new sample substrate (ie 25 mm coverslips vs 35 mm dishes) means a new
sample holder.  Thankfully that's easy to do on the 3D printer.

(Fluorescence) imaging at the same time is a bit of a kludge on our system,
but that is more reflective of the local group that designed it rather than
JPK.  I wouldn't doubt someone has a more elegant way to do that.

Thanks,
Rusty

On Fri, Aug 19, 2016 at 1:27 AM, Csúcs Gábor <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> For a project we are looking into the different possibilities to equip an
> existing microscope (Nikon Ti-Eclipse) with an optical tweezer system. Our
> primary application (right now) would be single cell manipulation (moving
> them) but an existing future possibility for an (easy) upgrade for a force
> measurement system would be also appreciated. Does anyone have experience
> with commercial systems? During my search I have found solutions from
> Thorlabs, JPK, Elliot scientific, Aresis, MMI and Zeiss (though for a Nikon
> scope this is probably not an option). Is there any other source existing?
> ). It would be also nice if the tweezer could be used in combination with
> Micromanager (used for imaging). Some posts smention also Arry but this
> company seem to disappeared. Thanks for any feedback!
>
> Greetings       Gabor
>

--

*Philip R Nicovich*

*Research Fellow,  **ARC Centre of Excellence in Advanced Molecular Imaging*



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