Compare TIRF microscopy geometries: prism-, lightguide-, and objective-based TIRF [commercial posting]

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Compare TIRF microscopy geometries: prism-, lightguide-, and objective-based TIRF [commercial posting]

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Dear Colleagues:

TIRF Labs is now offering for sale prism-based TIRF and lightguide-based TIRF microscopy
systems as add-on accessories for research microscopes. These geometries yield clean TIRF
effect with optical sectioning down to 20-40 nm. The evanescent wave decays exponentially,
unlike in objective-type TIRF, in which the evanescent wave is contaminated with large
intensity of stray light. For details visit www.tirf-labs.com  or www.TIRFmicroscopy.com.

In lgTIRF and pTIRF geometries, the excitation light is naturally independent from the
emission channel, which results in negligibly small intensity of stray light. The evanescent
wave is reproducible, TIRF images are crisp, and the signal-to-noise ratio is super high. You
can use dry, water- and oil-immersion objectives and perform TIRFing with UV excitation.
Several research groups have generated unique data using lgTIRF and pTIRF systems
http://tirf-labs.com/1st_pages_12reprints.pdf.  

In contrast to lg- and p-TIRF, the objective-type TIRF uses the emission channel to deliver
the excitation light to the TIRF interface. Large deviations from the theoretically predicted
exponential decay of the evanescent wave have been documented in the literature.  If you
have already acquired an oTIRF system, the analysis of sources will help you to mitigate the
problem of stray light: http://tirf-labs.com/microscopy.html.

Until the end of June 2016, we offer lg- and p-TIRF systems at promotional prices. We also
offer turnkey TIRF stations with service contracts. Contact us for more information.  Thank
you!


Best regards,
Alexander N. Asanov, Ph.D.
President,  TIRF Labs
106 Grendon Place
Cary, NC 27519
Tel: 919-463-9545   www.tirf-labs.com   www.TIRFmicroscopy.com
Mobile:  919-903-4792  [hidden email]