Compare TIRF microscopy geometries: prism-, lightguide-, and objective-based TIRF [commercial posting]

[Alex Asanov]

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Dear Colleagues:

 

TIRF Labs is now offering for sale prism-based TIRF and lightguide-based
TIRF microscopy systems as add-on accessories for research microscopes.
These geometries yield clean TIRF effect with optical sectioning down to
20-40 nm. The evanescent wave decays exponentially, unlike in objective-type
TIRF, in which the evanescent wave is contaminated with large intensity of
stray light. For details visit www.tirf-labs.com  or www.TIRFmicroscopy.com.


 

In lgTIRF and pTIRF geometries, the excitation light is naturally
independent from the emission channel, which results in negligibly small
intensity of stray light. The evanescent wave is reproducible, TIRF images
are crisp, and the signal-to-noise ratio is super high. You can use dry,
water- and oil-immersion objectives and perform TIRFing with UV excitation.
Several research groups have generated unique data using lgTIRF and pTIRF
systems http://tirf-labs.com/1st_pages_12reprints.pdf.  

 

In contrast to lg- and p-TIRF, the objective-type TIRF uses the emission
channel to deliver the excitation light to the TIRF interface. Large
deviations from the theoretically predicted exponential decay of the
evanescent wave have been documented in the literature.  If you have already
acquired an oTIRF system, the analysis of sources will help you to mitigate
the problem of stray light: http://tirf-labs.com/microscopy.html.

 

Until the end of June 2016, we offer lg- and p-TIRF systems at promotional
prices. Contact us for more information.

 

Best regards,

Alexander N. Asanov, Ph.D.
President,  TIRF Labs
106 Grendon Place
Cary, NC 27519
Tel: [phone]    <http://www.tirf-labs.com/> www.tirf-labs.com
<http://www.tirfmicroscopy.com/> www.TIRFmicroscopy.com
Mobile:  [phone]   <mailto:[hidden email]>
[hidden email]

 



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