Comparing Zeiss BiG detectors with Confocor APD detectors for FCS

[Ann-W]

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Dear Listsers

 

We’re considering implementing FCS however it turns out Zeiss are no longer selling their Confocor systems with APD detectors. Has anyone carried out comparisons between the GaSP detectors in the BiG detector or elsewhere on a Zeiss system with the APDs in the Confocor systems? If there are other analyses between these and other types of detectors I’d also be happy to hear about that or peoples experiences with other detectors as well. Please PM me.

Thanks

Ann

 

Dr Ann Wheeler
Advanced Imaging Resource, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU

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W: http://www.igmm.ac.uk/imaging.htm

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[0000001ed7f52e4a-dmarc-request]

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Hi Ann,

I have not done this comparison, but worked on a home built system with single photon counting APDs and on a LSM780 using internal GaAsP detector. This was for single molecules floating freely in solution, the SPAPDs were very much superior, but it was possible to do this with the internal detectors in photon counting mode. I gave up because I had to realign the pinhole every time for the FCCS experiments. Keep in mind that APD manufacturers usually give photon detection efficiency as specs, but for microscopes it is usually only the quantum efficiency of the detector material which is talked about.

Best wishes

Andreas

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From: [hidden email]
Sent: ‎14/‎10/‎2016 10:07
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Subject: Comparing Zeiss BiG detectors with Confocor APD detectors for FCS

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****

Dear Listsers

 

We’re considering implementing FCS however it turns out Zeiss are no longer selling their Confocor systems with APD detectors. Has anyone carried out comparisons between the GaSP detectors in the BiG detector or elsewhere on a Zeiss system with the APDs in the Confocor systems? If there are other analyses between these and other types of detectors I’d also be happy to hear about that or peoples experiences with other detectors as well. Please PM me.

Thanks

Ann

 

Dr Ann Wheeler
Advanced Imaging Resource, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU

E: [hidden email]
T: 0131 651 8665
W: http://www.igmm.ac.uk/imaging.htm

[George McNamara]

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****

Hi Ann,

I suggest contacting Ben at ISS and Wolfgang at B&H for their experiences and recommendations upgrading a Zeiss to FCS/FCCS (and maybe nowadays not just two color cross correlation, but triple, quadruple, etc?).

ISS (started with Prof. Gregorio Weber, who was a long time leader in fluorescence)

http://www.iss.com/microscopy/components/zeiss_FLIM_FCS.html

B&H - started by Wolfgang Becker - link below is titled FLIM, page 5 is FCS

http://www.becker-hickl.de/pdf/zeiss-big2-FLIM-03-web.pdf

George

p.s. to anyone interesting in a nice primer on F-techniques:

https://www.ncbi.nlm.nih.gov/pubmed/18387308

Trends Endocrinol Metab. 2008 Jul;19(5):181-90. doi: 10.1016/j.tem.2008.02.004. Epub 2008 Apr 1.
The F-techniques: advances in receptor protein studies.
Liu P1, Ahmed S, Wohland T.
Author information
Abstract
Recent developments in advanced microscopy techniques, the so-called F-techniques, including Förster resonance energy transfer, fluorescence correlation spectroscopy and fluorescence lifetime imaging, have led to a wide range of novel applications in biology. The F-techniques provide quantitative information on biomolecules and their interactions and give high spatial and temporal resolution. In particular, their application to receptor protein studies has led to new insights into receptor localization, oligomerization, activation and function in vivo. This review focuses on the application of the F-techniques to the study of receptor molecules and mechanisms in the last three years and provides information on new modalities that will further improve their applicability and widen the range of biological questions that can be addressed.
PMID: 18387308 DOI: 10.1016/j.tem.2008.02.004

On 10/14/2016 4:01 AM, WHEELER Ann wrote:

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****

Dear Listsers

 

We’re considering implementing FCS however it turns out Zeiss are no longer selling their Confocor systems with APD detectors. Has anyone carried out comparisons between the GaSP detectors in the BiG detector or elsewhere on a Zeiss system with the APDs in the Confocor systems? If there are other analyses between these and other types of detectors I’d also be happy to hear about that or peoples experiences with other detectors as well. Please PM me.

Thanks

Ann

 

Dr Ann Wheeler
Advanced Imaging Resource, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU

E: [hidden email]
T: 0131 651 8665
W: http://www.igmm.ac.uk/imaging.htm

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.