Configuration problem with Zeiss LSM 510
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G. Esteban Fernandez |
Re: Configuration problem with Zeiss LSM 510
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You need to go into the Maintain > Pinhole menu and optimize the X/Y positions of pinhole 3. The message means that has never been done for that particular configuration of dichroics. The pinhole is probably not centered directly in front of the PMT so you don't get a good image if it is closed down, but you'll probably get an image if you open the pinhole to max.
-Esteban
On Tue, Feb 10, 2009 at 9:54 AM, yee z <[hidden email]> wrote:
-- G. Esteban Fernandez, Ph.D. Associate Director Molecular Cytology Core Facility University of Missouri 120 Bond Life Sciences Center Columbia, MO 65211 http://www.biotech.missouri.edu/mcc/ 573-882-4895 573-884-9395 fax |
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Pascal Lorentz-2 |
Re: Configuration problem with Zeiss LSM 510
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In reply to this post by yee z
Hi Yi,
this is basically not a problem, it's normal behavior. You have to adjust the pinhole for each configuration. As Esteban already said this can be done in the Maintain > Pinhole menu. You have to center the pinhole in front of the detector. In most of the cases you will anyway get an image even if the pinhole is not adjusted but it might be of uneven brightness. Cheers Pascal -- Pascal Lorentz Department of Biomedicine University of Basel Mattenstrasse 28 4058 Basel Switzerland yee z schrieb: > Dear listers, > > We have a Zeiss LSM510 confocal microscopy system at our core > facility. And I got some problems while writing new configuration. > Here is an example: > Single track configuartion for FITC, I used to use HFT > 405/488/543/633, NFT 545, NFT 490 and BP505-550. It works fine. > (Screen shot: http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1A.jpg) > > But when I try to change the primary dichroic beam splitter to HFT > 488/594 and keep everything else the same, the systme shows "Pinhole > PH3 is not adjusted". (Screen shot: > http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1B.jpg) > > Also if I change the NFT 545 to NFT 595, the system also shows > "Pinhole PH3 is not adjusted" (Screen shot: > http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1C.jpg) > I am still be able to take the images regardless the error window. > > So does anyone here have the same problem? What does "Pinhole PH3 is > not adjusted" mean, any reason for this? > > Any suggestion will be appreciated. > > Thanks, > > Yi > > > |
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Boswell, Carl A - (cboswell) |
Re: Configuration problem with Zeiss LSM 510
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In reply to this post by G. Esteban Fernandez
All I can add to what Estaban said was be sure to
Save the position after you have made the adjustments. Adjust the image
Gain using the Palatte>Range Indicator so there is some saturation in it so
that you have an objective way to see if your changes are making things brighter
or dimmer. Adjusting the columator may also improve your
image.
Carl
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709
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Keith Morris |
Re: Configuration problem with Zeiss LSM 510
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In reply to this post by Boswell, Carl A - (cboswell)
Hi
Yes the 'pinhole not adjusted' warning always appears if you create a 'new' optical filter configuration. Although you can manually adjust the confocal pinhole [even incorrectly] to kill off the nagging & annoying message on the LMS510, Zeiss do provide a utility to do this automatically for you for all the filter configurations you have. You do this while focused on the mirrored slide Zeiss kindly gave you with the confocal when you bought it, and you run a little program that automatically aligns all the pinholes for all the optical configurations you use [even all those that don't display the warning message]. You switch on the 633nm laser select the 10x objective [always]. Leave the system to warm up for at least a couple of hours. Put the mirrored slide under the 10x and focus on it normally you can see dust which helps or use the palettes to show over exposure [red] and when its brightest youve hit the mirror. You then run PHAdjust.exe from Zeisss AIM folder [C:\aim\hwt\PHAdjust.exe on our PC]. This will automatically adjust all the available beam paths in your filter config files. For any other users with unusual filter configurations just resuse the beam path from one of their images and save it in your configs. Generally I run PHAjust.exe. every month. It takes about an hour to auto-adjust all the optics correctly and as a bonus gets rid of that annoying message. Regards Keith --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/cytogenetics/ ________________________________________ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Carl Boswell Sent: 10 February 2009 17:17 To: [hidden email] Subject: Re: Configuration problem with Zeiss LSM 510 All I can add to what Estaban said was be sure to Save the position after you have made the adjustments. Adjust the image Gain using the Palatte>Range Indicator so there is some saturation in it so that you have an objective way to see if your changes are making things brighter or dimmer. Adjusting the columator may also improve your image. Carl Carl A. Boswell, Ph.D. Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709 ----- Original Message ----- From: G. Esteban Fernandez To: [hidden email] Sent: Tuesday, February 10, 2009 9:12 AM Subject: Re: Configuration problem with Zeiss LSM 510 You need to go into the Maintain > Pinhole menu and optimize the X/Y positions of pinhole 3. The message means that has never been done for that particular configuration of dichroics. The pinhole is probably not centered directly in front of the PMT so you don't get a good image if it is closed down, but you'll probably get an image if you open the pinhole to max. -Esteban On Tue, Feb 10, 2009 at 9:54 AM, yee z <[hidden email]> wrote: Dear listers, We have a Zeiss LSM510 confocal microscopy system at our core facility. And I got some problems while writing new configuration. Here is an example: Single track configuartion for FITC, I used to use HFT 405/488/543/633, NFT 545, NFT 490 and BP505-550. It works fine. (Screen shot: http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1A.jpg) But when I try to change the primary dichroic beam splitter to HFT 488/594 and keep everything else the same, the systme shows "Pinhole PH3 is not adjusted". (Screen shot: http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1B.jpg) Also if I change the NFT 545 to NFT 595, the system also shows "Pinhole PH3 is not adjusted" (Screen shot: http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1C.jpg) I am still be able to take the images regardless the error window. So does anyone here have the same problem? What does "Pinhole PH3 is not adjusted" mean, any reason for this? Any suggestion will be appreciated. Thanks, Yi -- G. Esteban Fernandez, Ph.D. Associate Director Molecular Cytology Core Facility University of Missouri 120 Bond Life Sciences Center Columbia, MO 65211 http://www.biotech.missouri.edu/mcc/ 573-882-4895 573-884-9395 fax |
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Csúcs Gábor |
Spinning disk systems with FRAP possibility
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Dear List,
We are considering to purchase a spinning disk system with a FRAP/photoactivation module. I'm aware of the systems offered by Andor, PerkinElmer and Zeiss. Are you aware of any alternative provider? Thanks Gabor -- Gabor Csucs Light Microscopy Centre, ETH Zurich Schafmattstrasse 18, HPM F16 CH-8093, Zurich, Switzerland Web: www.lmc.ethz.ch Phone: +41 44 633 6221 Fax: +41 44 632 1298 e-mail: [hidden email] |
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Michael Weber-4 |
Re: Spinning disk systems with FRAP possibility
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Gabor,
3I (Intelligent Imaging Innovations) once told me that they would also be able to build such a setup. But I never had the chance to see it working. But maybe it's an option for you. Michael Gabor Csucs wrote: > Dear List, > > We are considering to purchase a spinning disk system with a > FRAP/photoactivation module. I'm aware of the systems offered by Andor, > PerkinElmer and Zeiss. > Are you aware of any alternative provider? > > Thanks Gabor |
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Beat Ludin |
Re: Spinning disk systems with FRAP possibility
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In reply to this post by Csúcs Gábor
Hi Gabor
We are just installing two spinning disk systems running under Metamorph here in Basel (Biozentrum and FMI), one of which will be equipped with a MAG FRAP module later on. The (dis-)advantage is that it is on open system, i.e. it is more flexible/extensible but presumably less integrated than the other systems. Let me know if you are interested. Cheers, Beat At 11:36 18-02-2009, Gabor Csucs wrote: >Content-Transfer-Encoding: 7bit > >Dear List, > >We are considering to purchase a spinning disk system with a >FRAP/photoactivation module. I'm aware of the systems offered by >Andor, PerkinElmer and Zeiss. >Are you aware of any alternative provider? > >Thanks Gabor > >-- >Gabor Csucs Light Microscopy Centre, ETH Zurich >Schafmattstrasse 18, HPM F16 CH-8093, Zurich, Switzerland > >Web: www.lmc.ethz.ch >Phone: +41 44 633 6221 >Fax: +41 44 632 1298 >e-mail: [hidden email] |
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Wiegraebe, Winfried-2 |
Re: Configuration problem with Zeiss LSM 510
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In reply to this post by Keith Morris
Some additions to the well described pinhole alignment procedure for the Zeiss LSM 510 described by Keith:
- I typically have a much better success rate if I use a glass slide instead of the mirror slide. The reflected light from the mirror tends to saturate the detectors. - PHAdjust adjusts only single tracks for the logged-in user. Thus we have one account where we collect all potential configurations and use this log-in to align the pinholes. We include reflected light configurations for the META detector to ensure proper alignment (they do not make any sense for normal imaging). Don't worry about duplicate configurations, PHAdust will care about them. - Before saving a configuration, I make sure that detector gain and offset are in a reasonable range (e.g. using Find). Otherwise PhAdjust might crash because the signal is to high or low. Hope this helps, Winfried ======================================= Winfried Wiegraebe Director, Stowers Microscopy Center Stowers Institute for Medical Research 1000 East 50th Street, Kansas City, MO 64110 http://research.stowers-institute.org/wiw/index.htm [hidden email] 816-926-4415 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Keith Morris Sent: Thursday, February 12, 2009 4:15 AM To: [hidden email] Subject: Re: Configuration problem with Zeiss LSM 510 Hi Yes the 'pinhole not adjusted' warning always appears if you create a 'new' optical filter configuration. Although you can manually adjust the confocal pinhole [even incorrectly] to kill off the nagging & annoying message on the LMS510, Zeiss do provide a utility to do this automatically for you for all the filter configurations you have. You do this while focused on the 'mirrored' slide Zeiss kindly gave you with the confocal when you bought it, and you run a little program that automatically aligns all the pinholes for all the optical configurations you use [even all those that don't display the warning message]. You switch on the 633nm laser select the 10x objective [always]. Leave the system to warm up for at least a couple of hours. Put the mirrored slide under the 10x and focus on it - normally you can see dust which helps or use the palettes to show over exposure [red] and when it's brightest you've hit the mirror. You then run PHAdjust.exe from Zeiss's AIM folder [C:\aim\hwt\PHAdjust.exe on our PC]. This will automatically adjust all the available beam paths in your filter config files. For any other users with unusual filter configurations just 'resuse' the beam path from one of their images and save it in your configs. Generally I run PHAjust.exe. every month. It takes about an hour to auto-adjust all the optics correctly and as a bonus gets rid of that annoying message. Regards Keith --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/cytogenetics/ ________________________________________ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Carl Boswell Sent: 10 February 2009 17:17 To: [hidden email] Subject: Re: Configuration problem with Zeiss LSM 510 All I can add to what Estaban said was be sure to Save the position after you have made the adjustments. Adjust the image Gain using the Palatte>Range Indicator so there is some saturation in it so that you have an objective way to see if your changes are making things brighter or dimmer. Adjusting the columator may also improve your image. Carl Carl A. Boswell, Ph.D. Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709 ----- Original Message ----- From: G. Esteban Fernandez To: [hidden email] Sent: Tuesday, February 10, 2009 9:12 AM Subject: Re: Configuration problem with Zeiss LSM 510 You need to go into the Maintain > Pinhole menu and optimize the X/Y positions of pinhole 3. The message means that has never been done for that particular configuration of dichroics. The pinhole is probably not centered directly in front of the PMT so you don't get a good image if it is closed down, but you'll probably get an image if you open the pinhole to max. -Esteban On Tue, Feb 10, 2009 at 9:54 AM, yee z <[hidden email]> wrote: Dear listers, We have a Zeiss LSM510 confocal microscopy system at our core facility. And I got some problems while writing new configuration. Here is an example: Single track configuartion for FITC, I used to use HFT 405/488/543/633, NFT 545, NFT 490 and BP505-550. It works fine. (Screen shot: http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1A.jpg) But when I try to change the primary dichroic beam splitter to HFT 488/594 and keep everything else the same, the systme shows "Pinhole PH3 is not adjusted". (Screen shot: http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1B.jpg) Also if I change the NFT 545 to NFT 595, the system also shows "Pinhole PH3 is not adjusted" (Screen shot: http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1C.jpg) I am still be able to take the images regardless the error window. So does anyone here have the same problem? What does "Pinhole PH3 is not adjusted" mean, any reason for this? Any suggestion will be appreciated. Thanks, Yi -- G. Esteban Fernandez, Ph.D. Associate Director Molecular Cytology Core Facility University of Missouri 120 Bond Life Sciences Center Columbia, MO 65211 http://www.biotech.missouri.edu/mcc/ 573-882-4895 573-884-9395 fax |
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Sylvie Le Guyader-2 |
Re: Spinning disk systems with FRAP possibility
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In reply to this post by Csúcs Gábor
Hi Gabor
We have such a system by Andor. It also has a spinning disk. I can certainly provide you with feedback on our machine if you need it. As far I as know, 3I works in collaboration with Zeiss on their TIRF/FRAP but that was early last year so it might have changed. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Dept of Biosciences and Nutrition Karolinska Institutet Novum 14157 Huddinge Sweden +46 (0)8 608 9240 > -----Original Message----- > From: Confocal Microscopy List > [mailto:] On Behalf Of Gabor Csucs > Sent: 18 February 2009 11:36 > To: [hidden email] > Subject: Spinning disk systems with FRAP possibility > > Dear List, > > We are considering to purchase a spinning disk system with a > FRAP/photoactivation module. I'm aware of the systems offered by Andor, > PerkinElmer and Zeiss. > Are you aware of any alternative provider? > > Thanks Gabor > > -- > Gabor Csucs > Light Microscopy Centre, ETH Zurich > Schafmattstrasse 18, HPM F16 > CH-8093, Zurich, Switzerland > > Web: www.lmc.ethz.ch > Phone: +41 44 633 6221 > Fax: +41 44 632 1298 > e-mail: [hidden email] |
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Keith Morris |
Re: Posting twice to Listserver. Posting twice to Listserver
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In reply to this post by Wiegraebe, Winfried-2
Hi all,
I notice that my messages are posting twice [and this is happening to other Listserver posters]. When I send a reply to 'confocal listserver' I get two messages returned, one saying OK distributed and one saying you have submitted the message twice and it's being rejected [it's a lying weasel, I only submitted it once]. Sometimes, sometimes not, the message appears twice on the list - once two weeks later, with an email on the day to me saying 'successfully listed' [again]. As the posting repeat is only happening to a few of us apparently, any ideas on what is going wrong? Am I registered twice somehow? There doesn't seem to be a God on this listserver [unlike microscopy.com] to chat to, and looking though the Listserver website wasn't enlightening. Searching the list-server archives only came up with old posters having the same problems, no answer to stop it. Thanks for your time. Keith --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/cytogenetics/ |
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Knecht, David |
Re: Posting twice to Listserver. Posting twice to Listserver
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Same thing is happening to me. Dave
On Mar 11, 2009, at 8:36 AM, Keith Morris wrote:
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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Jerry Sedgewick-2 |
Re: Posting twice to Listserver. Posting twice to Listserver
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Hello All,
The "real" administrator of the listserv (I'm the "adminstrator" insofar as I can remove or add names) wrote this note to me in regard to the message about posting twice in regard to an inquiry: "I see mail coming in from [hidden email] to [hidden email] and then 10 minutes later a mail coming into the [hidden email] from [hidden email] Is it possible that you are also sending to this list and then that list is sending back to the [hidden email] ?" It seems that if a subscriber was formerly subscribed to the server at Buffalo that hosted the listserv, then a second email gets bounced back from it because it is no longer operating. The email reads as follows: Your message is being returned to you unprocessed because it appears to have already been distributed to the CONFOCALMICROSCOPY list. That is, a message with identical text (but possibly with different mail headers) has been posted to the list recently, either by you or by someone else. If you have reason to resend this message to the list (for instance because you have been notified of a hardware failure with loss of data), please alter the text of the message in some way and resend it to the list. Altering the "Subject:" line or adding blank lines at the top or bottom of the message is not sufficient; instead, you should add a sentence or two at the top explaining why you are resending the message. This explanation will help the other subscribers understand why they are getting two copies of the same message. It is not possible to unsubscribe from the Buffalo list because it isn't operating as far as I know. I will see if there is anything else I can do from my end. In the meantime, ignore the message. Jerry Sedgewick David Knecht wrote: > Same thing is happening to me. Dave > > On Mar 11, 2009, at 8:36 AM, Keith Morris wrote: > >> Hi all, >> >> I notice that my messages are posting twice [and this is happening to >> other >> Listserver posters]. >> >> When I send a reply to 'confocal listserver' I get two messages returned, >> one saying OK distributed and one saying you have submitted the message >> twice and it's being rejected [it's a lying weasel, I only submitted it >> once]. Sometimes, sometimes not, the message appears twice on the list - >> once two weeks later, with an email on the day to me saying 'successfully >> listed' [again]. >> >> As the posting repeat is only happening to a few of us apparently, >> any ideas >> on what is going wrong? Am I registered twice somehow? There doesn't >> seem to >> be a God on this listserver [unlike microscopy.com] to chat to, and >> looking >> though the Listserver website wasn't enlightening. Searching the >> list-server >> archives only came up with old posters having the same problems, no >> answer >> to stop it. >> >> Thanks for your time. >> >> Keith >> >> --------------------------------------------------------------------------- >> Dr Keith J. Morris, >> Molecular Cytogenetics and Microscopy Core, >> Laboratory 00/069 and 00/070, >> The Wellcome Trust Centre for Human Genetics, >> Roosevelt Drive, >> Oxford OX3 7BN, >> United Kingdom. >> >> Telephone: +44 (0)1865 287568 >> Email: [hidden email] <mailto:[hidden email]> >> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ >> > > Dr. David Knecht > Department of Molecular and Cell Biology > Co-head Flow Cytometry and Confocal Microscopy Facility > U-3125 > 91 N. Eagleville Rd. > University of Connecticut > Storrs, CT 06269 > 860-486-2200 > 860-486-4331 (fax) > > -- Jerry (Gerald) Sedgewick Program Director, Biomedical Image Processing Lab (BIPL) Department of Neuroscience, University of Minnesota 312 Church St. SE, 1-205 Hasselmo Hall Minneapolis, MN 55455 (612) 624-6607 [hidden email] http://www.bipl.umn.edu Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output." Rawlight.com (dba Sedgewick Initiatives) 965 Cromwell Avenue Saint Paul, MN 55114 [hidden email] (651) 308-1466 http://www.quickphotoshop.com http://www.heartFROMstone.com http://www.rawlight.com --- Get FREE High Speed Internet from USFamily.Net! -- http://www.usfamily.net/mkt-freepromo.html --- |
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We are possibly getting close to the source of the problem.
There doesn't seem to be any evidence that it only happens to people who were subscribed to the old list - I think it happens to everyone so if there is anyone who has NOT had this double posting problem could they please reply? The problem was already happening before the transfer of ownership of the list. But since an alias was created (I presume) so that messages sent to the old list address would go to the new one (to make the transition seamless) any problem that preexisted would still be present .... Can someone please check the list of subscribers to [hidden email] to see if it contains [hidden email] ? This is a simple question which can be answered in a minute or less. I'll try a test to the latter address and see what happens. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jerry Sedgewick Sent: Thursday, 12 March 2009 3:49 AM To: [hidden email] Subject: Re: Posting twice to Listserver. Posting twice to Listserver Hello All, The "real" administrator of the listserv (I'm the "adminstrator" insofar as I can remove or add names) wrote this note to me in regard to the message about posting twice in regard to an inquiry: "I see mail coming in from [hidden email] to [hidden email] and then 10 minutes later a mail coming into the [hidden email] from [hidden email] Is it possible that you are also sending to this list and then that list is sending back to the [hidden email] ?" It seems that if a subscriber was formerly subscribed to the server at Buffalo that hosted the listserv, then a second email gets bounced back from it because it is no longer operating. The email reads as follows: Your message is being returned to you unprocessed because it appears to have already been distributed to the CONFOCALMICROSCOPY list. That is, a message with identical text (but possibly with different mail headers) has been posted to the list recently, either by you or by someone else. If you have reason to resend this message to the list (for instance because you have been notified of a hardware failure with loss of data), please alter the text of the message in some way and resend it to the list. Altering the "Subject:" line or adding blank lines at the top or bottom of the message is not sufficient; instead, you should add a sentence or two at the top explaining why you are resending the message. This explanation will help the other subscribers understand why they are getting two copies of the same message. It is not possible to unsubscribe from the Buffalo list because it isn't operating as far as I know. I will see if there is anything else I can do from my end. In the meantime, ignore the message. Jerry Sedgewick David Knecht wrote: > Same thing is happening to me. Dave > > On Mar 11, 2009, at 8:36 AM, Keith Morris wrote: > >> Hi all, >> >> I notice that my messages are posting twice [and this is happening to >> other Listserver posters]. >> >> When I send a reply to 'confocal listserver' I get two messages >> returned, one saying OK distributed and one saying you have submitted >> the message twice and it's being rejected [it's a lying weasel, I >> only submitted it once]. Sometimes, sometimes not, the message >> appears twice on the list - once two weeks later, with an email on >> the day to me saying 'successfully listed' [again]. >> >> As the posting repeat is only happening to a few of us apparently, >> any ideas on what is going wrong? Am I registered twice somehow? >> There doesn't seem to be a God on this listserver [unlike >> microscopy.com] to chat to, and looking though the Listserver website >> wasn't enlightening. Searching the list-server archives only came up >> with old posters having the same problems, no answer to stop it. >> >> Thanks for your time. >> >> Keith >> >> --------------------------------------------------------------------- >> ------ >> Dr Keith J. Morris, >> Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and >> 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt >> Drive, Oxford OX3 7BN, United Kingdom. >> >> Telephone: +44 (0)1865 287568 >> Email: [hidden email] <mailto:[hidden email]> >> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ >> > > Dr. David Knecht > Department of Molecular and Cell Biology Co-head Flow Cytometry and > Confocal Microscopy Facility > U-3125 > 91 N. Eagleville Rd. > University of Connecticut > Storrs, CT 06269 > 860-486-2200 > 860-486-4331 (fax) > > -- Jerry (Gerald) Sedgewick Program Director, Biomedical Image Processing Lab (BIPL) Department of Neuroscience, University of Minnesota 312 Church St. SE, 1-205 Hasselmo Hall Minneapolis, MN 55455 (612) 624-6607 [hidden email] http://www.bipl.umn.edu Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output." Rawlight.com (dba Sedgewick Initiatives) 965 Cromwell Avenue Saint Paul, MN 55114 [hidden email] (651) 308-1466 http://www.quickphotoshop.com http://www.heartFROMstone.com http://www.rawlight.com --- Get FREE High Speed Internet from USFamily.Net! -- http://www.usfamily.net/mkt-freepromo.html --- No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.10/1995 - Release Date: 11/03/2009 8:28 AM No virus found in this outgoing message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.10/1995 - Release Date: 11/03/2009 8:28 AM |
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Rosemary.White |
Re: Posting twice to Listserver. Posting twice to Listserver
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On 12/03/09 7:12 PM, "Guy Cox" <[hidden email]> wrote:
> We are possibly getting close to the source of the problem. > There doesn't seem to be any evidence that it only happens to > people who were subscribed to the old list - I think it happens > to everyone so if there is anyone who has NOT had this double > posting problem could they please reply? Doesn't happen to me, not for a long time.... cheers, Rosemary > > The problem was already happening before the transfer of ownership > of the list. But since an alias was created (I presume) so that > messages sent to the old list address would go to the new one (to > make the transition seamless) any problem that preexisted would > still be present .... > > Can someone please check the list of subscribers to > [hidden email] > to see if it contains [hidden email] ? This is a simple > question > which can be answered in a minute or less. > > I'll try a test to the latter address and see what happens. > > Guy > > > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of Jerry Sedgewick > Sent: Thursday, 12 March 2009 3:49 AM > To: [hidden email] > Subject: Re: Posting twice to Listserver. Posting twice to Listserver > > Hello All, > > The "real" administrator of the listserv (I'm the "adminstrator" insofar as I > can remove or add names) wrote this note to me in regard to the message about > posting twice in regard to an inquiry: > > "I see mail coming in from [hidden email] to > [hidden email] and then 10 minutes later a mail coming into > the [hidden email] from [hidden email] > > Is it possible that you are also sending to this list and then that list is > sending back to the [hidden email] ?" > > > It seems that if a subscriber was formerly subscribed to the server at Buffalo > that hosted the listserv, then a second email gets bounced back from it > because it is no longer operating. The email reads as follows: > > Your message is being returned to you unprocessed because it appears to > have already been distributed to the CONFOCALMICROSCOPY list. That is, a > message with identical text (but possibly with different mail headers) has > been posted to the list recently, either by you or by someone else. If you > have reason to resend this message to the list (for instance because you have > been notified of a hardware failure with loss of data), please alter the text > of the message in some way and resend it to the list. Altering the > "Subject:" line or adding blank lines at the top or bottom of the message is > not sufficient; instead, you should add a sentence or two at the top > explaining why you are resending the message. This explanation will help the > other subscribers understand why they are getting two copies of the same > message. > > It is not possible to unsubscribe from the Buffalo list because it isn't > operating as far as I know. I will see if there is anything else I can do > from my end. In the meantime, ignore the message. > > Jerry Sedgewick > > > > > > > David Knecht wrote: >> Same thing is happening to me. Dave >> >> On Mar 11, 2009, at 8:36 AM, Keith Morris wrote: >> >>> Hi all, >>> >>> I notice that my messages are posting twice [and this is happening to >>> other Listserver posters]. >>> >>> When I send a reply to 'confocal listserver' I get two messages >>> returned, one saying OK distributed and one saying you have submitted >>> the message twice and it's being rejected [it's a lying weasel, I >>> only submitted it once]. Sometimes, sometimes not, the message >>> appears twice on the list - once two weeks later, with an email on >>> the day to me saying 'successfully listed' [again]. >>> >>> As the posting repeat is only happening to a few of us apparently, >>> any ideas on what is going wrong? Am I registered twice somehow? >>> There doesn't seem to be a God on this listserver [unlike >>> microscopy.com] to chat to, and looking though the Listserver website >>> wasn't enlightening. Searching the list-server archives only came up >>> with old posters having the same problems, no answer to stop it. >>> >>> Thanks for your time. >>> >>> Keith >>> >>> --------------------------------------------------------------------- >>> ------ >>> Dr Keith J. Morris, >>> Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and >>> 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt >>> Drive, Oxford OX3 7BN, United Kingdom. >>> >>> Telephone: +44 (0)1865 287568 >>> Email: [hidden email] <mailto:[hidden email]> >>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ >>> >> >> Dr. David Knecht >> Department of Molecular and Cell Biology Co-head Flow Cytometry and >> Confocal Microscopy Facility >> U-3125 >> 91 N. Eagleville Rd. >> University of Connecticut >> Storrs, CT 06269 >> 860-486-2200 >> 860-486-4331 (fax) >> >> > > > -- > Jerry (Gerald) Sedgewick > Program Director, Biomedical Image Processing Lab (BIPL) Department of > Neuroscience, University of Minnesota > 312 Church St. SE, 1-205 Hasselmo Hall > Minneapolis, MN 55455 > (612) 624-6607 > [hidden email] > http://www.bipl.umn.edu > Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output." > > Rawlight.com (dba Sedgewick Initiatives) > 965 Cromwell Avenue > Saint Paul, MN 55114 > [hidden email] > (651) 308-1466 > http://www.quickphotoshop.com > http://www.heartFROMstone.com > http://www.rawlight.com > > > > > --- Get FREE High Speed Internet from USFamily.Net! -- > http://www.usfamily.net/mkt-freepromo.html --- > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.10/1995 - Release Date: 11/03/2009 > 8:28 AM > > > No virus found in this outgoing message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.10/1995 - Release Date: 11/03/2009 > 8:28 AM > |
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