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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi everyone, I made a mailing-list backup for the Confocal List at Nabble.com. I use it a lot and I thought some of you might be interested in using it also : http://www.nabble.com/Confocal-Microscopy-List-f25581.html It allows to interact with the list in a way similar to a forum, with search capabilities and a clean interface (a bit more friendly than the official archives). Of course it only contains email since I set it up (two weeks ago) so searching is not very helpful yet. But it allows to browse and send messages, and also suscribe to the list via an RSS feed. -- Christophe Leterrier Postdoc INSERM UMR641 Neurobiologie des canaux ioniques (Michael Seagar) Equipe Canaux ioniques et polarité neuronale (Bénédicte Dargent) IFR Jean Roche - Université de la Méditerranée 51, bd Pierre Dramard 13916 Marseille cedex 20 France /http://ifrjr.nord.univ-mrs.fr// tel: (33) 4 91 69 89 30 fax: (33) 4 91 09 05 06 /[hidden email] <mailto:[hidden email]> / |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I tried Nabble and it works great for me.
On Aug 27, 2007, at 10:30 AM, Christophe Leterrier wrote:
Thomas Baginski Director of IMAGING, room G-230 USUHS-Biomedical Instrumentation Center 4301 Jones Bridge Road Bethesda, MD 20814-4799 OFFICE: 301 295 5691 FAX: 301 319 8218 |
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In reply to this post by lechristophe
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I tried Nabble
and it works great for me.
On Aug 27, 2007, at 10:30 AM, Christophe Leterrier wrote:
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi everyone, I made a mailing-list backup for the Confocal List at Nabble.com. I use it a lot and I thought some of you might be interested in using it also : http://www.nabble.com/Confocal-Microscopy-List-f25581.html It allows to interact with the list in a way similar to a forum, with search capabilities and a clean interface (a bit more friendly than the official archives). Of course it only contains email since I set it up (two weeks ago) so searching is not very helpful yet. But it allows to browse and send messages, and also suscribe to the list via an RSS feed. -- Christophe Leterrier Postdoc INSERM UMR641 Neurobiologie des canaux ioniques (Michael Seagar) Equipe Canaux ioniques et polarité neuronale (Bénédicte Dargent) IFR Jean Roche - Université de la Méditerranée 51, bd Pierre Dramard 13916 Marseille cedex 20 France /http://ifrjr.nord.univ-mrs.fr// tel: (33) 4 91 69 89 30 fax: (33) 4 91 09 05 06 /[hidden email] <[hidden email]> / Thomas Baginski
Director of IMAGING, room G-230
USUHS-Biomedical Instrumentation Center
4301 Jones Bridge Road
Bethesda, MD 20814-4799
OFFICE: 301 295 5691
FAX: 301 319 8218
-- ****************************************
Prof. James B. Pawley, Room 223, Zoology Research Building, FAX 608-262-9083 250 N. Mills St., Madison, WI, 53706 [hidden email] "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39 |
 
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Lauran Oomen |
Single Q-dots for PSF measurement
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In reply to this post by Thomas Baginski
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello
all,
We have
tried to use Q-dots for PSF measurements on our confocals, but in our
hands they are not bright enough and also bleach. I guess a number of you
have tried Q-Dots as well, since they promise to be good
sub-resolution targets. I have searched the archive and only found a message
about this dating back to 2004, also reporting negative results. Did anyone
successfully use Q-dots as sub-resolution targets to measure the PSF of a
confocal? If yes, could you share your protocol with us?
Lauran
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Eric Scarfone |
Re: Single Q-dots for PSF measurement
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal hello if I remember well, Q-dots are remarquably bright using multiphoton excitation (check out the original Zipfel paper). In my hands, using continuous light sources, they are bright but not that much. I never tried to dilute them that much that I could visualise subresolution dots. Eric Eric Scarfone, PhD, CNRS, Center for Hearing and communication Research Department of Clinical Neuroscience Karolinska Institutet Postal Address: CFH, M1:02 Karolinska Hospital, SE-171 76 Stockholm, Sweden Work: +46 (0)8-517 70343, Cell: +46 (0)70 888 2352 Fax: +46 (0)8-301876 email: [hidden email] http://www.ki.se/cfh/ ----- Original Message ----- From: Lauran Oomen <[hidden email]> Date: Thursday, August 30, 2007 11:35 am Subject: Single Q-dots for PSF measurement To: [hidden email] > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hello all, > > We have tried to use Q-dots for PSF measurements on our confocals, > but in our hands they are not bright enough and also bleach. I > guess a number of you have tried Q-Dots as well, since they promise > to be good sub-resolution targets. I have searched the archive and > only found a message about this dating back to 2004, also reporting > negative results. Did anyone successfully use Q-dots as sub- > resolution targets to measure the PSF of a confocal? If yes, could > you share your protocol with us? > > Lauran > ************************************* > Lauran Oomen > The Netherlands Cancer Institute > Digital Microscopy Facility (H0) > PO box 90203 > 1006BE Amsterdam > The Netherlands > tel: +31 20 5126080 > fax: +31 20 5122909 > > |
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Gary Laevsky-2 |
Re: Single Q-dots for PSF measurement
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I've looked at Qdots using 2P, 1P, and widefield and they have always been pretty bright. Can the "blinking" phenomena be an issue? Do they even still do that? Best, Gary Laevsky, Ph.D. Imaging Application Specialist Andor Technology discover new ways of seeing Cell (774) 291 - 9992 Office (860) 290 - 9211 x219 Fax (860) 290 - 9566 Web: www.andor.com -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Eric Scarfone Sent: Thursday, August 30, 2007 11:14 AM To: [hidden email] Subject: Re: Single Q-dots for PSF measurement Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal hello if I remember well, Q-dots are remarquably bright using multiphoton excitation (check out the original Zipfel paper). In my hands, using continuous light sources, they are bright but not that much. I never tried to dilute them that much that I could visualise subresolution dots. Eric Eric Scarfone, PhD, CNRS, Center for Hearing and communication Research Department of Clinical Neuroscience Karolinska Institutet Postal Address: CFH, M1:02 Karolinska Hospital, SE-171 76 Stockholm, Sweden Work: +46 (0)8-517 70343, Cell: +46 (0)70 888 2352 Fax: +46 (0)8-301876 email: [hidden email] http://www.ki.se/cfh/ ----- Original Message ----- From: Lauran Oomen <[hidden email]> Date: Thursday, August 30, 2007 11:35 am Subject: Single Q-dots for PSF measurement To: [hidden email] > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hello all, > > We have tried to use Q-dots for PSF measurements on our confocals, > but in our hands they are not bright enough and also bleach. I > guess a number of you have tried Q-Dots as well, since they promise > to be good sub-resolution targets. I have searched the archive and > only found a message about this dating back to 2004, also reporting > negative results. Did anyone successfully use Q-dots as sub- > resolution targets to measure the PSF of a confocal? If yes, could > you share your protocol with us? > > Lauran > ************************************* > Lauran Oomen > The Netherlands Cancer Institute > Digital Microscopy Facility (H0) > PO box 90203 > 1006BE Amsterdam > The Netherlands > tel: +31 20 5126080 > fax: +31 20 5122909 > > |
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Eli Rothenberg |
Re: Single Q-dots for PSF measurement
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In reply to this post by Lauran Oomen
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal You can suppress blinking, and have long lived surface attached single Qdots in solution- should be no problem. What's good about Qdots that you can excite them with any wavelength below their emission. Here are several references, for blinking suppression of dyes and Qdots, and for localization using single fluorophores. 1)Hyokeun Park, George Hanson, Steven Duff, Paul Selvin. Nanometer Localization of Single ReAsH Molecules. Journal of Microscopy, 216, 199-205(2004). 2)Rasnik, I., S. A. McKinney, and T. Ha, "Nonblinking and Long-lasting Single Molecule Fluorescence Imaging", Nature Methods 3(11), 891-893 (2006) 3)Hohng S., and T. Ha, "Near-complete Suppression of Quantum Dot Blinking in Ambient Conditions", J. Am. Chem. Soc. 126(5), 1324-1325 (2004) good luck Eli _______________ Eli Rothenberg Postdoctoral research associate HHMI lab and the department of physics University of Illinois Urbana-Champaign ---- Original message ---- >Date: Thu, 30 Aug 2007 11:25:05 +0200 >From: Lauran Oomen <[hidden email]> >Subject: Single Q-dots for PSF measurement >To: [hidden email] > > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > Hello all, > > We have tried to use Q-dots for PSF measurements > on our confocals, but in our hands they are > not bright enough and also bleach. I guess a number > of you have tried Q-Dots as well, since they promise > to be good sub-resolution targets. I have searched > the archive and only found a message about this > dating back to 2004, also reporting negative > results. Did anyone successfully use Q-dots as > sub-resolution targets to measure the PSF of a > confocal? If yes, could you share your protocol with > us? > > Lauran > > ************************************* > Lauran Oomen > The Netherlands Cancer Institute > Digital Microscopy Facility (H0) > PO box 90203 > 1006BE Amsterdam > The Netherlands > tel: +31 20 5126080 > fax: +31 20 5122909 |
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Matt Bootman |
Re: Single Q-dots for PSF measurement
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal <vendor response> Try a sample of QD nanoclusters from Crystalplex. Nanoclusters are aggregates of multiple QD's encased in a water soluble shell. They are approx. 25 nm (+/-10 nm) in diameter. They are much brighter than individual quantum dots and the signal from multiple dots averages out blinking. Matt Matt Bootman CEO Crystalplex Corporation 890 William Pitt Way Pittsburgh, PA 15238 http://www.crystalplex.com 412.826.3081 412.826.3083 fx 724.344.7128 cell -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Eli Rothenberg Sent: Thursday, August 30, 2007 12:25 PM To: [hidden email] Subject: Re: Single Q-dots for PSF measurement Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal You can suppress blinking, and have long lived surface attached single Qdots in solution- should be no problem. What's good about Qdots that you can excite them with any wavelength below their emission. Here are several references, for blinking suppression of dyes and Qdots, and for localization using single fluorophores. 1)Hyokeun Park, George Hanson, Steven Duff, Paul Selvin. Nanometer Localization of Single ReAsH Molecules. Journal of Microscopy, 216, 199-205(2004). 2)Rasnik, I., S. A. McKinney, and T. Ha, "Nonblinking and Long-lasting Single Molecule Fluorescence Imaging", Nature Methods 3(11), 891-893 (2006) 3)Hohng S., and T. Ha, "Near-complete Suppression of Quantum Dot Blinking in Ambient Conditions", J. Am. Chem. Soc. 126(5), 1324-1325 (2004) good luck Eli _______________ Eli Rothenberg Postdoctoral research associate HHMI lab and the department of physics University of Illinois Urbana-Champaign ---- Original message ---- >Date: Thu, 30 Aug 2007 11:25:05 +0200 >From: Lauran Oomen <[hidden email]> >Subject: Single Q-dots for PSF measurement >To: [hidden email] > > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > Hello all, > > We have tried to use Q-dots for PSF measurements > on our confocals, but in our hands they are > not bright enough and also bleach. I guess a number > of you have tried Q-Dots as well, since they promise > to be good sub-resolution targets. I have searched > the archive and only found a message about this > dating back to 2004, also reporting negative > results. Did anyone successfully use Q-dots as > sub-resolution targets to measure the PSF of a > confocal? If yes, could you share your protocol with > us? > > Lauran > > ************************************* > Lauran Oomen > The Netherlands Cancer Institute > Digital Microscopy Facility (H0) > PO box 90203 > 1006BE Amsterdam > The Netherlands > tel: +31 20 5126080 > fax: +31 20 5122909 |
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