tag:confocal-microscopy-list.275.s1.nabble.com,2006:forum-588098Nabble - Confocal Microscopy List2024-03-29T07:56:24ZThis is a mirror of the Confocal Microscopy Mailing List.
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<br/><br/>Dear All,
<br/><br/>We are happy to announce the new iteration of Switzerland's Image and Data Analysis School, ZIDAS 2021! Once again, EPFL and ETHZ have teamed up to co-organize this summer school with an emphasis on the incredible tools created by this very community that makes this forum so great.
<br/><br/>This year we are still playing it safe and running the event online, hoping to be back on-site next year.
<br/><br/>This one-week school provides a hands-on introduction to image processing and analysis, with an emphasis on biologically relevant examples.
<br/><br/>This school is for you if...
<br/><br/> * you are a life-science researcher with a pressing need to quantify your light-microscopy images.
<br/> * you are uncertain about how to: Best calculate co-localisation, do deconvolution, automate the counting of cells, track objects over time, handle massive amounts of image data, record your image-analysis workflows in a reproducible manner.
<br/><br/>More information can be found here:
<br/><a href="https://2021.zidas.org/" target="_top" rel="nofollow" link="external">https://2021.zidas.org/</a><br/><br/>We are looking forward to welcoming you soon!
<br/>The organization team
<br/>Arne, Olivier, Romain, Simon & Szymon
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592368Re: uniform focus across the field question2021-06-16T10:41:47Z2021-06-16T10:41:47ZZdenek Svindrych-2
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<br/><br/>Or (seconding to Steffen), does the microscope stand have another pot where
<br/>you could connect a camera directly?
<br/>As already mentioned, if your sample does not go too much out of focus when
<br/>you move the stage a long distance (1 cm) then you can't fix your issue
<br/>with stage insert leveling screws. The issue then must be somewhere between
<br/>the objective lens and the camera (filters in the main turret are not
<br/>suspect, as these are in transmission and in infinity space; thanks Dan for
<br/>pointing out the nosepiece).
<br/>Alternatively, the whole XY stage is not bolted correctly to the microscope
<br/>frame.
<br/>zdenek
<br/><br/>On Wed, Jun 16, 2021 at 12:51 PM Steffen Dietzel <<a href="/user/SendEmail.jtp?type=node&node=7592368&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/>wrote:
<br/><div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
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<br/>> *****
<br/>>
<br/>> Hi Michael,
<br/>>
<br/>> does your microscope have eye pieces? If so, checking for a flat field
<br/>> with the eye pieces should solve the issue whether the tilt comes from
<br/>> the camera alignment or from the stage, no? Beads on a glass surface or
<br/>> any other thin sample should be a good sample for this.
<br/>>
<br/>> Also, how does the tilt behave with different objectives, say 40x and
<br/>> 100x? You should get a different outcome depending on whether the
<br/>> problems arise from the sample/stage or behind the intermediate image.
<br/>>
<br/>> Good hunting!
<br/>>
<br/>> Steffen
<br/>>
<br/>> Am 16.06.2021 um 03:21 schrieb Cammer, Michael:
<br/>> > *****
<br/>> > To join, leave or search the confocal microscopy listserv, go to:
<br/>> > <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> > Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your
<br/>> posting.
<br/>> > *****
<br/>> >
<br/>> > Dear confocalers and other light microscopists,
<br/>> >
<br/>> > We have a question about tilt of a specimen or detectors leading to
<br/>> shift of focus across a field.
<br/>> >
<br/>> > >From the first confocal microscope we used in 1991 through many other
<br/>> microscopes over the years, we have not had problems with planapochromat
<br/>> lenses with the image being in focus corner to corner across the field.
<br/>> This includes newer microscopes with wider fields of view and a spinning
<br/>> disk confocal with a Cairn twin camera setup. (The cameras, however,
<br/>> required substantial alignment to attain this, but it has been locked in
<br/>> place for over a year.) We are used to putting in standard stage inserts
<br/>> or even unusual ones, a variety of samples, and being able to focus at the
<br/>> substrate across the entire field. This includes imaging in reflection
<br/>> mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent
<br/>> confocal. This is routine.
<br/>> >
<br/>> > We have a new microscope, however, which does not have this focus
<br/>> uniformity. I believe it is a dual camera alignment issue and we have been
<br/>> discussing this for almost a year as an installation issue, but recently
<br/>> the manufacturer insists that the only way to fix the problem we need to
<br/>> use screws on the stage to adjust the tilt. I am concerned that this is
<br/>> going to be a major problem in a core facility with users who will play
<br/>> with anything. It is going to add substantial burden on staff. Also, it
<br/>> means that standard inserts will not work. And it makes me wonder what
<br/>> this will do to the PSF if the coverglass isn't perpendicular to the light
<br/>> path.
<br/>> >
<br/>> > Have we been unusually lucky all these years to never have encountered
<br/>> this problem before? Are we unreasonable to expect the microscope to be
<br/>> installed with all planes parallel for routine use? Is this type of stage
<br/>> alignment standard acceptable procedure in other labs?
<br/>> >
<br/>> > Thank you.
<br/>> >
<br/>> >
<br/>> >
<br/>> > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/>> >
<br/>> > NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
<br/>> NY 10016
<br/>> >
<br/>> > <a href="/user/SendEmail.jtp?type=node&node=7592368&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592368&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/>> <a href="http://nyulmc.org/micros" target="_top" rel="nofollow" link="external">http://nyulmc.org/micros</a> <a href="http://microscopynotes.com/" target="_top" rel="nofollow" link="external">http://microscopynotes.com/</a><br/>> >
<br/>> > Voice direct only, no text or messages: 1-914-309-3270 and
<br/>> 1-646-501-0567
<br/>> >
<br/>> >
<br/>> > ------------------------------------------------------------
<br/>> > This email message, including any attachments, is for the sole use of
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<br/>> confidential, and exempt from disclosure under applicable law. Any
<br/>> unauthorized review, use, disclosure, or distribution is prohibited. If you
<br/>> have received this email in error please notify the sender by return email
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<br/>> transmitted by this email.
<br/>> > =================================
<br/>>
<br/>> --
<br/>> ------------------------------------------------------------
<br/>> Steffen Dietzel, PD Dr. rer. nat
<br/>> Ludwig-Maximilians-Universität München
<br/>> Biomedical Center (BMC)
<br/>> Head of the Core Facility Bioimaging
<br/>>
<br/>> Großhaderner Straße 9
<br/>> D-82152 Planegg-Martinsried
<br/>> Germany
<br/>>
<br/>> <a href="http://www.bioimaging.bmc.med.uni-muenchen.de" target="_top" rel="nofollow" link="external">http://www.bioimaging.bmc.med.uni-muenchen.de</a><br/>>
</div><br/><br/>--
<br/>--
<br/>Zdenek Svindrych, Ph.D.
<br/>Research Scientist - Microscopy Imaging Specialist
<br/>Department of Biochemistry and Cell Biology
<br/>Geisel School of Medicine at Dartmouth
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592367Job opening (commercial): Super-resolution applications specialist at Bruker2021-06-16T10:30:02Z2021-06-16T10:30:02ZWinfried Wiegraebe-2
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<br/><br/>Dear all,
<br/>We have an open position for an expert in biological super-resolution microscopy to support our sales force in the US. You would work out of San Jose, CA, or Madison, WI.
<br/><br/>Responsibilities:
<br/>- Support sales, product management, and marketing teams
<br/>- Discuss with customers regarding applications, hardware, and software of the Vutara VXL system and perform remote and on-site demonstrations
<br/>- Help with system installation at customer site and train customers
<br/>- Support customers after sales with application development and instrument operation
<br/>- Work with customers, product management, and external collaborators to develop new application fields.
<br/>- Work with product management and marketing to create application notes and marketing material
<br/>- Give presentations about applications and system capabilities at customer sites, conferences, trade shows, and webinars
<br/>- The successful applicant might be responsible for setting up and maintaining an application laboratory
<br/><br/>We are looking for a candidate who enjoys interacting with biologists from world-class academic laboratories, troubleshoot applications, and represent Bruker at conferences and trade shows.
<br/><br/>For further information and to apply, go to:
<br/><a href="https://englishcareers-bruker.icims.com/jobs/10305/sales-applications-and-service-specialist/job" target="_top" rel="nofollow" link="external">https://englishcareers-bruker.icims.com/jobs/10305/sales-applications-and-service-specialist/job</a><br/><br/>Feel free to contact me directly at: <a href="/user/SendEmail.jtp?type=node&node=7592367&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/><br/>I am looking forward to your resume and please spread the word,
<br/>Winfried
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592366Re: uniform focus across the field question2021-06-16T09:50:08Z2021-06-16T09:50:08ZSteffen Dietzel
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<br/><br/>Hi Michael,
<br/><br/>does your microscope have eye pieces? If so, checking for a flat field
<br/>with the eye pieces should solve the issue whether the tilt comes from
<br/>the camera alignment or from the stage, no? Beads on a glass surface or
<br/>any other thin sample should be a good sample for this.
<br/><br/>Also, how does the tilt behave with different objectives, say 40x and
<br/>100x? You should get a different outcome depending on whether the
<br/>problems arise from the sample/stage or behind the intermediate image.
<br/><br/>Good hunting!
<br/><br/>Steffen
<br/><br/>Am 16.06.2021 um 03:21 schrieb Cammer, Michael:
<div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Dear confocalers and other light microscopists,
<br/>>
<br/>> We have a question about tilt of a specimen or detectors leading to shift of focus across a field.
<br/>>
<br/>> >From the first confocal microscope we used in 1991 through many other microscopes over the years, we have not had problems with planapochromat lenses with the image being in focus corner to corner across the field. This includes newer microscopes with wider fields of view and a spinning disk confocal with a Cairn twin camera setup. (The cameras, however, required substantial alignment to attain this, but it has been locked in place for over a year.) We are used to putting in standard stage inserts or even unusual ones, a variety of samples, and being able to focus at the substrate across the entire field. This includes imaging in reflection mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent confocal. This is routine.
<br/>>
<br/>> We have a new microscope, however, which does not have this focus uniformity. I believe it is a dual camera alignment issue and we have been discussing this for almost a year as an installation issue, but recently the manufacturer insists that the only way to fix the problem we need to use screws on the stage to adjust the tilt. I am concerned that this is going to be a major problem in a core facility with users who will play with anything. It is going to add substantial burden on staff. Also, it means that standard inserts will not work. And it makes me wonder what this will do to the PSF if the coverglass isn't perpendicular to the light path.
<br/>>
<br/>> Have we been unusually lucky all these years to never have encountered this problem before? Are we unreasonable to expect the microscope to be installed with all planes parallel for routine use? Is this type of stage alignment standard acceptable procedure in other labs?
<br/>>
<br/>> Thank you.
<br/>>
<br/>>
<br/>>
<br/>> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/>>
<br/>> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
<br/>>
<br/>> <a href="/user/SendEmail.jtp?type=node&node=7592366&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592366&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> <a href="http://nyulmc.org/micros" target="_top" rel="nofollow" link="external">http://nyulmc.org/micros</a> <a href="http://microscopynotes.com/" target="_top" rel="nofollow" link="external">http://microscopynotes.com/</a><br/>>
<br/>> Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
<br/>>
<br/>>
<br/>> ------------------------------------------------------------
<br/>> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
<br/>> =================================
</div><br/>--
<br/>------------------------------------------------------------
<br/>Steffen Dietzel, PD Dr. rer. nat
<br/>Ludwig-Maximilians-Universität München
<br/>Biomedical Center (BMC)
<br/>Head of the Core Facility Bioimaging
<br/><br/>Großhaderner Straße 9
<br/>D-82152 Planegg-Martinsried
<br/>Germany
<br/><br/><a href="http://www.bioimaging.bmc.med.uni-muenchen.de" target="_top" rel="nofollow" link="external">http://www.bioimaging.bmc.med.uni-muenchen.de</a><br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592365Re: uniform focus across the field question2021-06-16T08:52:19Z2021-06-16T08:52:19ZArmstrong, Brian
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<br/><br/>Hi Michael, for our Zeiss confocal with Airyscan we have a leveling stage insert that came with a protocol for using it. If you want to spend the time of a few minutes you can get a small field of view very flat. Some stage inserts are fairly uniform across vendors so perhaps this could be adapted to your system.
<br/><br/>Cheers,
<br/><br/>Brian Armstrong PhD
<br/>Director, Light Microscopy Core
<br/>Research Professor
<br/>Department of Shared Resources
<br/>Beckman Research Institute, City of Hope
<br/><br/>-----Original Message-----
<br/>From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592365&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>> On Behalf Of Cammer, Michael
<br/>Sent: Tuesday, June 15, 2021 6:21 PM
<br/>To: <a href="/user/SendEmail.jtp?type=node&node=7592365&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>Subject: uniform focus across the field question
<br/><br/>[Attention: This email came from an external source. Do not open attachments or click on links from unknown senders or unexpected emails.]
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<br/><br/>Dear confocalers and other light microscopists,
<br/><br/>We have a question about tilt of a specimen or detectors leading to shift of focus across a field.
<br/><br/>From the first confocal microscope we used in 1991 through many other microscopes over the years, we have not had problems with planapochromat lenses with the image being in focus corner to corner across the field. This includes newer microscopes with wider fields of view and a spinning disk confocal with a Cairn twin camera setup. (The cameras, however, required substantial alignment to attain this, but it has been locked in place for over a year.) We are used to putting in standard stage inserts or even unusual ones, a variety of samples, and being able to focus at the substrate across the entire field. This includes imaging in reflection mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent confocal. This is routine.
<br/><br/>We have a new microscope, however, which does not have this focus uniformity. I believe it is a dual camera alignment issue and we have been discussing this for almost a year as an installation issue, but recently the manufacturer insists that the only way to fix the problem we need to use screws on the stage to adjust the tilt. I am concerned that this is going to be a major problem in a core facility with users who will play with anything. It is going to add substantial burden on staff. Also, it means that standard inserts will not work. And it makes me wonder what this will do to the PSF if the coverglass isn't perpendicular to the light path.
<br/><br/>Have we been unusually lucky all these years to never have encountered this problem before? Are we unreasonable to expect the microscope to be installed with all planes parallel for routine use? Is this type of stage alignment standard acceptable procedure in other labs?
<br/><br/>Thank you.
<br/><br/><br/><br/>Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/><br/>NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
<br/><br/><a href="/user/SendEmail.jtp?type=node&node=7592365&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592365&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a>> <a href="https://urldefense.com/v3/__http://nyulmc.org/micros__;!!Fou38LsQmgU!7uabNHoQqyP5N8IeFqRTFkSCUkBUEwmikEqS8RboDGArNZjB8p0Z1EI5JB7D-Ww$" target="_top" rel="nofollow" link="external">https://urldefense.com/v3/__http://nyulmc.org/micros__;!!Fou38LsQmgU!7uabNHoQqyP5N8IeFqRTFkSCUkBUEwmikEqS8RboDGArNZjB8p0Z1EI5JB7D-Ww$</a> <a href="https://urldefense.com/v3/__http://microscopynotes.com/__;!!Fou38LsQmgU!7uabNHoQqyP5N8IeFqRTFkSCUkBUEwmikEqS8RboDGArNZjB8p0Z1EI5SWV_-GE$" target="_top" rel="nofollow" link="external">https://urldefense.com/v3/__http://microscopynotes.com/__;!!Fou38LsQmgU!7uabNHoQqyP5N8IeFqRTFkSCUkBUEwmikEqS8RboDGArNZjB8p0Z1EI5SWV_-GE$</a>
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592364Re: [External] uniform focus across the field question2021-06-16T06:32:38Z2021-06-16T06:32:38ZDouglas Richardson
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<br/><br/>Hi Michael,
<br/><br/>As a follow up to our previous conversation:
<br/><br/>This microscope (others can see the previous string, it's not a confocal)
<br/>comes standard with a unique levelling stage insert. It has X and Y
<br/>adjustment screws in opposite corners and a pivot point in the other
<br/>corner. This stage insert can correct for any tilt throughout the system,
<br/>although it can take a long time to get it right.
<br/><br/>Since you mentioned TIRF, I assume you're not using that insert, but a live
<br/>cell heated stage insert? I do not like the standard heated stage that
<br/>ships with that system. In our facility it eats objectives. Therefore, we
<br/>ordered the Pecon push and click system for live cell holders (and rely on
<br/>the plastic incubation box + time to maintain temperature). We had to sign
<br/>a waiver acknowledging that this insert was not approved for use with the
<br/>system and that we may experience unwanted thermal drift and uncorrectable
<br/>tilt.
<br/><br/>It turns out we didn't see any of this and now exclusively use the push and
<br/>click system for live and fixed samples as it can also hold slides. The
<br/>adjustable stage now collects dust in a box and our images look great. No
<br/>need for adjusting stage screws.
<br/><br/>To be fair, we don't do TIRF, but we image 200 um beads at every SIM
<br/>session and these are always in focus across the field. It was very
<br/>difficult to achieve similar uniformity with the adjustable stage unless
<br/>you put in a good 30-45 mins of adjustments.
<br/><br/>-Doug
<br/><br/>On Wed, Jun 16, 2021 at 9:16 AM Jonkman, James <<a href="/user/SendEmail.jtp?type=node&node=7592364&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/>wrote:
<br/><div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Hi, Michael. We have a dozen confocals and none of them have any
<br/>> appreciable focus shift across a single field of view. In fact, on the
<br/>> inverted microscopes with a thin sample if a user has a label close to one
<br/>> edge of the slide and no label on the other, this should cause an obvious
<br/>> tilt as the slide sits higher on one side than the other - but from a
<br/>> single field of view it isn't very obvious unless you're looking for it.
<br/>> The effect of the label becomes obvious if you move the stage - the sample
<br/>> quickly goes out of focus as you move side to side. If there is a
<br/>> substantial tilt across the field of view I would agree that the
<br/>> manufacturer should address this, and not with stage inserts: we usually
<br/>> remove the stage insert tilt screws as they tend to jiggle their way down
<br/>> with gravity and end up misaligning the stage tilt more than anything else.
<br/>>
<br/>> Maybe you want to create a shared cloud folder (Dropbox, Onedrive, ...)
<br/>> and drop an image or 2 in there so that we can see how bad it is? If it
<br/>> would help, I would be willing to take some images across several of our
<br/>> confocals and upload them to the same folder. I'm thinking a short z-stack
<br/>> of a thin sample (Molecular Probes Prepared Slide #1, BPAE cells for
<br/>> example) would illustrate the problem nicely. And maybe a second test
<br/>> would be to focus the same slide in the middle of the field of view, then
<br/>> take a tiled image (say, 5 x 5). Choose an objective and we can try to
<br/>> match it. Anyone else interested? If not I'm happy to discuss further
<br/>> offline.
<br/>>
<br/>> Cheers,
<br/>> James
<br/>>
<br/>> -----------------------------------------------
<br/>> James Jonkman, Staff Scientist
<br/>> Advanced Optical Microscopy Facility (AOMF)
<br/>> and Wright Cell Imaging Facility (WCIF)
<br/>> University Health Network
<br/>> MaRS, PMCRT tower, 101 College St., Room 15-305
<br/>> Toronto, ON, CANADA M5G 1L7
<br/>> <a href="/user/SendEmail.jtp?type=node&node=7592364&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a> Tel: 416-581-8593
<br/>> www.aomf.ca
<br/>>
<br/>>
<br/>> -----Original Message-----
<br/>> From: Confocal Microscopy List [mailto:<a href="/user/SendEmail.jtp?type=node&node=7592364&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>]
<br/>> On Behalf Of Cammer, Michael
<br/>> Sent: Tuesday, June 15, 2021 9:21 PM
<br/>> To: <a href="/user/SendEmail.jtp?type=node&node=7592364&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>> Subject: [External] uniform focus across the field question
<br/>>
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<br/>> *****
<br/>>
<br/>> Dear confocalers and other light microscopists,
<br/>>
<br/>> We have a question about tilt of a specimen or detectors leading to shift
<br/>> of focus across a field.
<br/>>
<br/>> From the first confocal microscope we used in 1991 through many other
<br/>> microscopes over the years, we have not had problems with planapochromat
<br/>> lenses with the image being in focus corner to corner across the field.
<br/>> This includes newer microscopes with wider fields of view and a spinning
<br/>> disk confocal with a Cairn twin camera setup. (The cameras, however,
<br/>> required substantial alignment to attain this, but it has been locked in
<br/>> place for over a year.) We are used to putting in standard stage inserts
<br/>> or even unusual ones, a variety of samples, and being able to focus at the
<br/>> substrate across the entire field. This includes imaging in reflection
<br/>> mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent
<br/>> confocal. This is routine.
<br/>>
<br/>> We have a new microscope, however, which does not have this focus
<br/>> uniformity. I believe it is a dual camera alignment issue and we have been
<br/>> discussing this for almost a year as an installation issue, but recently
<br/>> the manufacturer insists that the only way to fix the problem we need to
<br/>> use screws on the stage to adjust the tilt. I am concerned that this is
<br/>> going to be a major problem in a core facility with users who will play
<br/>> with anything. It is going to add substantial burden on staff. Also, it
<br/>> means that standard inserts will not work. And it makes me wonder what
<br/>> this will do to the PSF if the coverglass isn't perpendicular to the light
<br/>> path.
<br/>>
<br/>> Have we been unusually lucky all these years to never have encountered
<br/>> this problem before? Are we unreasonable to expect the microscope to be
<br/>> installed with all planes parallel for routine use? Is this type of stage
<br/>> alignment standard acceptable procedure in other labs?
<br/>>
<br/>> Thank you.
<br/>>
<br/>>
<br/>>
<br/>> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/>>
<br/>> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY
<br/>> 10016
<br/>>
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592363Re: [External] uniform focus across the field question2021-06-16T06:15:50Z2021-06-16T06:15:50ZJonkman, James
*****
<br/>To join, leave or search the confocal microscopy listserv, go to:
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<br/>*****
<br/><br/>Hi, Michael. We have a dozen confocals and none of them have any appreciable focus shift across a single field of view. In fact, on the inverted microscopes with a thin sample if a user has a label close to one edge of the slide and no label on the other, this should cause an obvious tilt as the slide sits higher on one side than the other - but from a single field of view it isn't very obvious unless you're looking for it. The effect of the label becomes obvious if you move the stage - the sample quickly goes out of focus as you move side to side. If there is a substantial tilt across the field of view I would agree that the manufacturer should address this, and not with stage inserts: we usually remove the stage insert tilt screws as they tend to jiggle their way down with gravity and end up misaligning the stage tilt more than anything else.
<br/><br/>Maybe you want to create a shared cloud folder (Dropbox, Onedrive, ...) and drop an image or 2 in there so that we can see how bad it is? If it would help, I would be willing to take some images across several of our confocals and upload them to the same folder. I'm thinking a short z-stack of a thin sample (Molecular Probes Prepared Slide #1, BPAE cells for example) would illustrate the problem nicely. And maybe a second test would be to focus the same slide in the middle of the field of view, then take a tiled image (say, 5 x 5). Choose an objective and we can try to match it. Anyone else interested? If not I'm happy to discuss further offline.
<br/><br/>Cheers,
<br/>James
<br/><br/>-----------------------------------------------
<br/> James Jonkman, Staff Scientist
<br/> Advanced Optical Microscopy Facility (AOMF)
<br/> and Wright Cell Imaging Facility (WCIF)
<br/> University Health Network
<br/> MaRS, PMCRT tower, 101 College St., Room 15-305
<br/> Toronto, ON, CANADA M5G 1L7
<br/> <a href="/user/SendEmail.jtp?type=node&node=7592363&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a> Tel: 416-581-8593
<br/> www.aomf.ca
<br/><br/><br/>-----Original Message-----
<br/>From: Confocal Microscopy List [mailto:<a href="/user/SendEmail.jtp?type=node&node=7592363&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>] On Behalf Of Cammer, Michael
<br/>Sent: Tuesday, June 15, 2021 9:21 PM
<br/>To: <a href="/user/SendEmail.jtp?type=node&node=7592363&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>Subject: [External] uniform focus across the field question
<br/><br/>*****
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<br/>*****
<br/><br/>Dear confocalers and other light microscopists,
<br/><br/>We have a question about tilt of a specimen or detectors leading to shift of focus across a field.
<br/><br/>From the first confocal microscope we used in 1991 through many other microscopes over the years, we have not had problems with planapochromat lenses with the image being in focus corner to corner across the field. This includes newer microscopes with wider fields of view and a spinning disk confocal with a Cairn twin camera setup. (The cameras, however, required substantial alignment to attain this, but it has been locked in place for over a year.) We are used to putting in standard stage inserts or even unusual ones, a variety of samples, and being able to focus at the substrate across the entire field. This includes imaging in reflection mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent confocal. This is routine.
<br/><br/>We have a new microscope, however, which does not have this focus uniformity. I believe it is a dual camera alignment issue and we have been discussing this for almost a year as an installation issue, but recently the manufacturer insists that the only way to fix the problem we need to use screws on the stage to adjust the tilt. I am concerned that this is going to be a major problem in a core facility with users who will play with anything. It is going to add substantial burden on staff. Also, it means that standard inserts will not work. And it makes me wonder what this will do to the PSF if the coverglass isn't perpendicular to the light path.
<br/><br/>Have we been unusually lucky all these years to never have encountered this problem before? Are we unreasonable to expect the microscope to be installed with all planes parallel for routine use? Is this type of stage alignment standard acceptable procedure in other labs?
<br/><br/>Thank you.
<br/><br/><br/><br/>Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/><br/>NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
<br/><br/><a href="/user/SendEmail.jtp?type=node&node=7592363&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592363&i=4" target="_top" rel="nofollow" link="external">[hidden email]</a>> <a href="https://urldefense.com/v3/__http://nyulmc.org/micros__;!!CjcC7IQ!Z7U94tM1sMtYZhhlvDZ6lwfd94ioWz9rYEzmfx_koBo2Yd27Vv0vHrKYYJniWk7qagUc$" target="_top" rel="nofollow" link="external">https://urldefense.com/v3/__http://nyulmc.org/micros__;!!CjcC7IQ!Z7U94tM1sMtYZhhlvDZ6lwfd94ioWz9rYEzmfx_koBo2Yd27Vv0vHrKYYJniWk7qagUc$</a> [nyulmc[.]org] <a href="https://urldefense.com/v3/__http://microscopynotes.com/__;!!CjcC7IQ!Z7U94tM1sMtYZhhlvDZ6lwfd94ioWz9rYEzmfx_koBo2Yd27Vv0vHrKYYJniWvaaQUMl$" target="_top" rel="nofollow" link="external">https://urldefense.com/v3/__http://microscopynotes.com/__;!!CjcC7IQ!Z7U94tM1sMtYZhhlvDZ6lwfd94ioWz9rYEzmfx_koBo2Yd27Vv0vHrKYYJniWvaaQUMl$</a> [microscopynotes[.]com]
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592362Re: uniform focus across the field question2021-06-16T04:09:58Z2021-06-16T04:09:58ZDan Focht
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<br/>*****
<br/><br/>Michael
<br/><br/><br/>As a former Zeiss affiliate service tech, back when I was young, and this still applies today, we used a stage square to make sure that the flat surface of the nosepiece and the surface of the stage were parallel with each other. The device was made on a lathe. The base was about 24mm diameter and screwed into the nosepiece and extended 45mm to a flat disk about 75mm round. You would carefully lower the 75mm disk to the stage surface to make sure it touched evenly. Can be used on uprights or inverts.
<br/>This enabled the user to check for parallelism issues caused by either the stage mount or the detent in the nosepiece. If the detent wore it caused a rotational misalignment which causes the focus to be nonuniform across the field. It is also best to have a crosshair objective to check alignment of the optical axis of the scope. Check with your scope manufacturer to see if they can or will provide these reference parts.
<br/><br/><br/><br/>Dan Focht
<br/><a href="/user/SendEmail.jtp?type=node&node=7592362&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/><br/><br/><div class='shrinkable-quote'><br/>> On Jun 15, 2021, at 9:21 PM, Cammer, Michael <<a href="/user/SendEmail.jtp?type=node&node=7592362&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> wrote:
<br/>>
<br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
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<br/>> *****
<br/>>
<br/>> Dear confocalers and other light microscopists,
<br/>>
<br/>> We have a question about tilt of a specimen or detectors leading to shift of focus across a field.
<br/>>
<br/>> From the first confocal microscope we used in 1991 through many other microscopes over the years, we have not had problems with planapochromat lenses with the image being in focus corner to corner across the field. This includes newer microscopes with wider fields of view and a spinning disk confocal with a Cairn twin camera setup. (The cameras, however, required substantial alignment to attain this, but it has been locked in place for over a year.) We are used to putting in standard stage inserts or even unusual ones, a variety of samples, and being able to focus at the substrate across the entire field. This includes imaging in reflection mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent confocal. This is routine.
<br/>>
<br/>> We have a new microscope, however, which does not have this focus uniformity. I believe it is a dual camera alignment issue and we have been discussing this for almost a year as an installation issue, but recently the manufacturer insists that the only way to fix the problem we need to use screws on the stage to adjust the tilt. I am concerned that this is going to be a major problem in a core facility with users who will play with anything. It is going to add substantial burden on staff. Also, it means that standard inserts will not work. And it makes me wonder what this will do to the PSF if the coverglass isn't perpendicular to the light path.
<br/>>
<br/>> Have we been unusually lucky all these years to never have encountered this problem before? Are we unreasonable to expect the microscope to be installed with all planes parallel for routine use? Is this type of stage alignment standard acceptable procedure in other labs?
<br/>>
<br/>> Thank you.
<br/>>
<br/>>
<br/>>
<br/>> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/>>
<br/>> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
<br/>>
<br/>> <a href="/user/SendEmail.jtp?type=node&node=7592362&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592362&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a>> <a href="http://nyulmc.org/micros" target="_top" rel="nofollow" link="external">http://nyulmc.org/micros</a> <a href="http://microscopynotes.com/" target="_top" rel="nofollow" link="external">http://microscopynotes.com/</a><br/>>
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<br/>>
<br/>>
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592361Re: uniform focus across the field question2021-06-15T18:49:15Z2021-06-15T18:49:15ZGeorge McNamara
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<br/><br/>Hi Michael,
<br/><br/>There is no tolerance on how level a microscope stage, or its specimen
<br/>holding insert (ex. K-Frame) is supposed to be for research microscopes.
<br/>In fact, other than #1.5 coverglasses being ~170 um of glass made of
<br/>[whatever] (ok, and similar specifications for #0, #1, #2, etc), there
<br/>are not a lot of specifications (and most of the specifications, re:
<br/>ISO, are just to try to cause innovators to be slowed down by writing
<br/>mountains of documentation).
<br/><br/>I suggest: get an "optical flat" (my local Olympus rep had one ... or
<br/>maybe it floats with some of O's demo equipment ... if too expensive,
<br/>then a plain glass slide, image, turn around 180 degrees, image, if
<br/>consistent, hope it is "flat" enough), and use reflected light -- for a
<br/>widefield filter set to do this see, for example, my
<br/><a href="http://confocal.jhu.edu/current-equipment/fishscope" target="_top" rel="nofollow" link="external">http://confocal.jhu.edu/current-equipment/fishscope</a> page (RT10/90 or
<br/>RT03/97 -- either one is fine for this, and for interference reflection
<br/>light microscope of cell-substratum interactions ... many confocals have
<br/>R10/T90 or similar master[mistress?] beamsplitter) -- to determine if
<br/>the two cameras simultaneously see the same thing or not (flat or not).
<br/>If not, then use the "stage screws" (i.e. the screws on the corners of a
<br/>K-frame insert) to level the insert with respect to one or hopefully
<br/>both. If only one is flat, follow whatever instructions the dual camera
<br/>thingy manufacturer recommends to get the other to match.
<br/><br/>This becomes the "ground truth" and users are then responsible for doing
<br/>their best to make their coverglass-slide and imaging dish purchases and
<br/>"assemblies" (ex: no label or coverglass at one edge of a slide) be flat
<br/>with respect to your "truth".
<br/><br/>Document all your steps ... including (hopefully) that that your
<br/>"optical flat" remains constant over days, weeks, months, then publish
<br/>one or more articles (or preprints, then articles, then protocol articles).
<br/><br/>enjoy,
<br/><br/>George
<br/><br/>p.s. except for TIRF filter cubes (and maybe not even then) the
<br/>fluorescence filter companies (i.e. Chroma, Semrock) do not claim their
<br/>glass is flat (or exactly 45 degrees for beamsplitters ... hmmm ... some
<br/>companies may deliberately tilt their emission filters from being
<br/>exactly perpendicular to the light path), so different filter cubes
<br/>could impact the microscope flatness ... thin layer of fluorescent stuff
<br/>(or small beads) or Michael Model's approach of high concentration
<br/>fluorescent dyes could be useful.
<br/><br/>p.p.s. "you have another filter(s)" - whatever is splitting the light
<br/>path between the two cameras may not have perfectly "optical flat"
<br/>glasses. Maybe that component should have a TIRF quality beamsplitter
<br/>and high quality emission filters.
<br/><br/>p.p.s. having (or buying on amazon.com, Home Depot, etc) a "bubble
<br/>level" could tell you if your microscope stage is level wrt gravity. I
<br/>suggest no need for something like "BLACK+DECKER Laser Level (BDL220S)"
<br/>(amazon.com product, currently $14.52) ... if you do buy a laser
<br/>leveler, please keep in mind: "do not look at laser with remaining eye".
<br/><br/><br/>On 6/15/2021 9:21 PM, Cammer, Michael wrote:
<div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Dear confocalers and other light microscopists,
<br/>>
<br/>> We have a question about tilt of a specimen or detectors leading to shift of focus across a field.
<br/>>
<br/>> From the first confocal microscope we used in 1991 through many other microscopes over the years, we have not had problems with planapochromat lenses with the image being in focus corner to corner across the field. This includes newer microscopes with wider fields of view and a spinning disk confocal with a Cairn twin camera setup. (The cameras, however, required substantial alignment to attain this, but it has been locked in place for over a year.) We are used to putting in standard stage inserts or even unusual ones, a variety of samples, and being able to focus at the substrate across the entire field. This includes imaging in reflection mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent confocal. This is routine.
<br/>>
<br/>> We have a new microscope, however, which does not have this focus uniformity. I believe it is a dual camera alignment issue and we have been discussing this for almost a year as an installation issue, but recently the manufacturer insists that the only way to fix the problem we need to use screws on the stage to adjust the tilt. I am concerned that this is going to be a major problem in a core facility with users who will play with anything. It is going to add substantial burden on staff. Also, it means that standard inserts will not work. And it makes me wonder what this will do to the PSF if the coverglass isn't perpendicular to the light path.
<br/>>
<br/>> Have we been unusually lucky all these years to never have encountered this problem before? Are we unreasonable to expect the microscope to be installed with all planes parallel for routine use? Is this type of stage alignment standard acceptable procedure in other labs?
<br/>>
<br/>> Thank you.
<br/>>
<br/>>
<br/>>
<br/>> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/>>
<br/>> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
<br/>>
<br/>> <a href="/user/SendEmail.jtp?type=node&node=7592361&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592361&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> <a href="http://nyulmc.org/micros" target="_top" rel="nofollow" link="external">http://nyulmc.org/micros</a> <a href="http://microscopynotes.com/" target="_top" rel="nofollow" link="external">http://microscopynotes.com/</a><br/>>
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<br/>>
<br/>>
<br/>> ------------------------------------------------------------
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<br/>> =================================
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592360uniform focus across the field question2021-06-15T18:21:02Z2021-06-15T18:21:02ZCammer, Michael-2
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<br/>*****
<br/><br/>Dear confocalers and other light microscopists,
<br/><br/>We have a question about tilt of a specimen or detectors leading to shift of focus across a field.
<br/><br/>From the first confocal microscope we used in 1991 through many other microscopes over the years, we have not had problems with planapochromat lenses with the image being in focus corner to corner across the field. This includes newer microscopes with wider fields of view and a spinning disk confocal with a Cairn twin camera setup. (The cameras, however, required substantial alignment to attain this, but it has been locked in place for over a year.) We are used to putting in standard stage inserts or even unusual ones, a variety of samples, and being able to focus at the substrate across the entire field. This includes imaging in reflection mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent confocal. This is routine.
<br/><br/>We have a new microscope, however, which does not have this focus uniformity. I believe it is a dual camera alignment issue and we have been discussing this for almost a year as an installation issue, but recently the manufacturer insists that the only way to fix the problem we need to use screws on the stage to adjust the tilt. I am concerned that this is going to be a major problem in a core facility with users who will play with anything. It is going to add substantial burden on staff. Also, it means that standard inserts will not work. And it makes me wonder what this will do to the PSF if the coverglass isn't perpendicular to the light path.
<br/><br/>Have we been unusually lucky all these years to never have encountered this problem before? Are we unreasonable to expect the microscope to be installed with all planes parallel for routine use? Is this type of stage alignment standard acceptable procedure in other labs?
<br/><br/>Thank you.
<br/><br/><br/><br/>Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
<br/><br/>NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
<br/><br/><a href="/user/SendEmail.jtp?type=node&node=7592360&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592360&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> <a href="http://nyulmc.org/micros" target="_top" rel="nofollow" link="external">http://nyulmc.org/micros</a> <a href="http://microscopynotes.com/" target="_top" rel="nofollow" link="external">http://microscopynotes.com/</a><br/><br/>Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
<br/><br/><br/>------------------------------------------------------------
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592359Re: MyScope is down. Any info?2021-06-15T02:07:52Z2021-06-15T02:07:52ZAurora
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<br/>*****
<br/><br/>Dear All,
<br/><br/>thank you so much for the feedback and the help!
<br/>Apparently the problem was that our firewall blacklisted the website.
<br/>Also in a neighbour institute (i.e. not same network) there was this
<br/>problem, therefore we assumed it was a global issue.
<br/>So thanks for everyone that gave the feedback "for me it's working"!
<br/><br/>Thank you (and a big sorry!) to Michelle Peckham for the email
<br/>addresses. With the stress of the moment I did not think about running a
<br/>search query through the confocalmailinglist.... #facepalm
<br/><br/>Taking the chance to wish you all a lovely day
<br/>cheers, Aurora
<br/><br/>On 14/06/2021 17:14, Christoph Ruediger Bauer wrote:
<div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Dear Aurora,
<br/>>
<br/>> I know and used the "myscope" site for teaching and especially liked the TEM simulator. But it was based on the Flash Player which was deprecated in 2017 and officially discontinued on December 31, 2020. They state this equally on the myscope website: "Due to the discontinued support of Flash, the TEM and XRD simulators are currently unavailable". I think other pages and resources are still accessible.
<br/>> Best regards,
<br/>> Christoph
<br/>>
<br/>>
<br/>> PHOTONIC Bioimaging Center
<br/>> & Dubochet Center for Imaging CryoGEnic
<br/>> University of Geneva - Science II
<br/>> Room 245
<br/>> 30, Quai Ernest Ansermet
<br/>> CH - 1211 Genève 4
<br/>>
<br/>> Dr. Christoph R. Bauer
<br/>> Managing Director
<br/>> Tel.: + 41 22 3796632
<br/>> Fax: + 41 22 3796868
<br/>> email: <a href="/user/SendEmail.jtp?type=node&node=7592359&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>> websites: <a href="https://www.unige.ch/bioimaging/photonic/" target="_top" rel="nofollow" link="external">https://www.unige.ch/bioimaging/photonic/</a><br/>> &: <a href="https://cryoem.unige.ch" target="_top" rel="nofollow" link="external">https://cryoem.unige.ch</a><br/>>
<br/>>
<br/>>
<br/>> -----Original Message-----
<br/>> From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592359&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> On Behalf Of Aurora Panzera
<br/>> Sent: 14 June 2021 12:29
<br/>> To: <a href="/user/SendEmail.jtp?type=node&node=7592359&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>> Subject: MyScope is down. Any info?
<br/>>
<br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Dear Microscopy people.
<br/>>
<br/>> The online learning environment MyScope <a href="https://myscope.training" target="_top" rel="nofollow" link="external">https://myscope.training</a> is down since this morning.
<br/>>
<br/>> Does anyone have any news about whether the resource will be available later today?
<br/>>
<br/>> We, Light Microscopy Facility of the Max Planck Tübingen, are currently running a course for the master Students of Biochemistry:
<br/>> The plan for this week is that students in their afternoon are self-learning a certain Module from the MyScope which we facility-people will then discuss and demonstrate on the day after.
<br/>>
<br/>> If this resource won't be available for the next day(s) we will need to restructure our course.
<br/>> I couldn't find an email address of the developers therefore I am writing here to see if someone can give us some news.
<br/>>
<br/>> Otherwise, do you know if we can get some of the modules as a PDF(or other) and how?
<br/>>
<br/>>
<br/>> thanking you all
<br/>>
<br/>> Bests
<br/>> Aurora
<br/>>
<br/>> --
<br/>> Aurora Panzera (she/her)
<br/>> Light Microscopy Facility
<br/>> Max-Planck-Institute for Developmental Biology Max-Planck-Ring 5
<br/>> D-72076 Tuebingen
<br/>> Germany
<br/>>
<br/>> Phone: +49 7071 601 1443
<br/>> e-mail:<a href="/user/SendEmail.jtp?type=node&node=7592359&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a>
</div><br/>--
<br/>Aurora Panzera (she/her)
<br/>Light Microscopy Facility
<br/>Max-Planck-Institute for Developmental Biology
<br/>Max-Planck-Ring 5
<br/>D-72076 Tuebingen
<br/>Germany
<br/><br/>Phone: +49 7071 601 1443
<br/>e-mail:<a href="/user/SendEmail.jtp?type=node&node=7592359&i=4" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592358Re: MyScope is down. Any info?2021-06-14T08:14:56Z2021-06-14T08:14:56ZChristoph Ruediger Bauer
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<br/>*****
<br/><br/>Dear Aurora,
<br/><br/>I know and used the "myscope" site for teaching and especially liked the TEM simulator. But it was based on the Flash Player which was deprecated in 2017 and officially discontinued on December 31, 2020. They state this equally on the myscope website: "Due to the discontinued support of Flash, the TEM and XRD simulators are currently unavailable". I think other pages and resources are still accessible.
<br/>Best regards,
<br/>Christoph
<br/><br/><br/>PHOTONIC Bioimaging Center
<br/>& Dubochet Center for Imaging CryoGEnic
<br/>University of Geneva - Science II
<br/>Room 245
<br/>30, Quai Ernest Ansermet
<br/>CH - 1211 Genève 4
<br/><br/>Dr. Christoph R. Bauer
<br/>Managing Director
<br/>Tel.: + 41 22 3796632
<br/>Fax: + 41 22 3796868
<br/>email: <a href="/user/SendEmail.jtp?type=node&node=7592358&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>websites: <a href="https://www.unige.ch/bioimaging/photonic/" target="_top" rel="nofollow" link="external">https://www.unige.ch/bioimaging/photonic/</a><br/>&: <a href="https://cryoem.unige.ch" target="_top" rel="nofollow" link="external">https://cryoem.unige.ch</a><br/><br/><br/><br/>-----Original Message-----
<br/>From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592358&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> On Behalf Of Aurora Panzera
<br/>Sent: 14 June 2021 12:29
<br/>To: <a href="/user/SendEmail.jtp?type=node&node=7592358&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>Subject: MyScope is down. Any info?
<br/><br/>*****
<br/>To join, leave or search the confocal microscopy listserv, go to:
<br/><a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>*****
<br/><br/> Dear Microscopy people.
<br/><br/>The online learning environment MyScope <a href="https://myscope.training" target="_top" rel="nofollow" link="external">https://myscope.training</a> is down since this morning.
<br/><br/>Does anyone have any news about whether the resource will be available later today?
<br/><br/>We, Light Microscopy Facility of the Max Planck Tübingen, are currently running a course for the master Students of Biochemistry:
<br/>The plan for this week is that students in their afternoon are self-learning a certain Module from the MyScope which we facility-people will then discuss and demonstrate on the day after.
<br/><br/>If this resource won't be available for the next day(s) we will need to restructure our course.
<br/>I couldn't find an email address of the developers therefore I am writing here to see if someone can give us some news.
<br/><br/>Otherwise, do you know if we can get some of the modules as a PDF(or other) and how?
<br/><br/><br/>thanking you all
<br/><br/>Bests
<br/>Aurora
<br/><br/>--
<br/>Aurora Panzera (she/her)
<br/>Light Microscopy Facility
<br/>Max-Planck-Institute for Developmental Biology Max-Planck-Ring 5
<br/>D-72076 Tuebingen
<br/>Germany
<br/><br/>Phone: +49 7071 601 1443
<br/>e-mail:<a href="/user/SendEmail.jtp?type=node&node=7592358&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592357Re: MyScope is down. Any info?2021-06-14T05:25:43Z2021-06-14T05:25:43ZZdenek Svindrych-2
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<br/>*****
<br/><br/>Hi Aurora,
<br/><br/>thanks for pointing out this nice resource. It seems to work OK from New
<br/>Hampshire as of 6/14/2021, 8:20 am EST.
<br/><br/>Best, zdenek
<br/><br/>On Mon, Jun 14, 2021 at 6:40 AM Aurora Panzera <<a href="/user/SendEmail.jtp?type=node&node=7592357&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/>wrote:
<br/><div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Dear Microscopy people.
<br/>>
<br/>> The online learning environment MyScope <a href="https://myscope.training" target="_top" rel="nofollow" link="external">https://myscope.training</a> is down
<br/>> since this morning.
<br/>>
<br/>> Does anyone have any news about whether the resource will be available
<br/>> later today?
<br/>>
<br/>> We, Light Microscopy Facility of the Max Planck Tübingen, are currently
<br/>> running a course for the master Students of Biochemistry:
<br/>> The plan for this week is that students in their afternoon are
<br/>> self-learning a certain Module from the MyScope which we facility-people
<br/>> will then discuss and demonstrate on the day after.
<br/>>
<br/>> If this resource won't be available for the next day(s) we will need to
<br/>> restructure our course.
<br/>> I couldn't find an email address of the developers therefore I am writing
<br/>> here to see if someone can give us some news.
<br/>>
<br/>> Otherwise, do you know if we can get some of the modules as a PDF(or other)
<br/>> and how?
<br/>>
<br/>>
<br/>> thanking you all
<br/>>
<br/>> Bests
<br/>> Aurora
<br/>>
<br/>> --
<br/>> Aurora Panzera (she/her)
<br/>> Light Microscopy Facility
<br/>> Max-Planck-Institute for Developmental Biology
<br/>> Max-Planck-Ring 5
<br/>> D-72076 Tuebingen
<br/>> Germany
<br/>>
<br/>> Phone: +49 7071 601 1443
<br/>> e-mail:<a href="/user/SendEmail.jtp?type=node&node=7592357&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>>
</div><br/><br/>--
<br/>--
<br/>Zdenek Svindrych, Ph.D.
<br/>Research Scientist - Microscopy Imaging Specialist
<br/>Department of Biochemistry and Cell Biology
<br/>Geisel School of Medicine at Dartmouth
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592356FW: [EXTERNAL] Re: Confocal and Widefield microscopy training material2021-06-14T05:19:29Z2021-06-14T05:19:29ZMichelle Peckham
*****
<br/>To join, leave or search the confocal microscopy listserv, go to:
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<br/>*****
<br/><br/>And another possible contact?
<br/><br/>On 17/10/2019, 05:34, "Confocal Microscopy List on behalf of Neftali Flores Rodriguez" <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a> on behalf of <a href="/user/SendEmail.jtp?type=node&node=7592356&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> wrote:
<br/><br/> *****
<br/> To join, leave or search the confocal microscopy listserv, go to:
<br/> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/> *****
<br/><br/> Hi Niyanta,
<br/><br/> You might find our MyScope useful.
<br/><br/> <a href="https://myscope.training/" target="_top" rel="nofollow" link="external">https://myscope.training/</a><br/><br/><br/> Neftali Flores-Rodriguez | Light Microscopist
<br/><br/> Sydney Microscopy and Microanalysis (SMM)
<br/> Australian Centre for Microscopy & Microanalysis (ACMM)
<br/><br/> THE UNIVERSITY OF SYDNEY
<br/><br/><br/> ________________________________
<br/> From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>> on behalf of Kumar, Niyanta <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/> Sent: Wednesday, 16 October 2019, 6:04 pm
<br/> To: <a href="/user/SendEmail.jtp?type=node&node=7592356&i=4" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
<br/><br/> *****
<br/> To join, leave or search the confocal microscopy listserv, go to:
<br/> <a href="https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu</a><br/> Post images on <a href="https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com</a> and include the link in your posting.
<br/> *****
<br/><br/> Thanks Sylvie, this is helpful!
<br/><br/> Niyanta Kumar, Ph.D.
<br/> Senior Scientist - Pharmacokinetics and ADME
<br/> Merck Research Laboratories
<br/><br/> -----Original Message-----
<br/> From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=5" target="_top" rel="nofollow" link="external">[hidden email]</a>> On Behalf Of Sylvie Le Guyader
<br/> Sent: Wednesday, October 16, 2019 9:55 AM
<br/> To: <a href="/user/SendEmail.jtp?type=node&node=7592356&i=6" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
<br/><br/> EXTERNAL EMAIL – Use caution with any links or file attachments.
<br/><br/> *****
<br/> To join, leave or search the confocal microscopy listserv, go to:
<br/> <a href="https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu</a><br/> Post images on <a href="https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com</a> and include the link in your posting.
<br/> *****
<br/><br/> ’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’
<br/> Definitely! That would bore me too and I am not from the millennium generation! 😉
<br/> I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊
<br/><br/> Med vänlig hälsning / Best regards
<br/><br/> Sylvie
<br/><br/> @@@@@@@@@@@@@@@@@@@@@@@@
<br/> Sylvie Le Guyader, PhD
<br/> Live Cell Imaging Facility Manager
<br/> Karolinska Institutet- Bionut Dpt
<br/> Blickagången 16,
<br/> Room 7362 (lab)/7840 (office)
<br/> 14157 Huddinge, Sweden
<br/> mobile: +46 (0) 73 733 5008
<br/> LCI website<<a href="https://protect-au.mimecast.com/s/40xyCMwvLQTAQG37hJE2HF?domain=ki.se" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/40xyCMwvLQTAQG37hJE2HF?domain=ki.se</a>>
<br/> Follow our microscopy blog!<<a href="https://protect-au.mimecast.com/s/vt_4CNLwM9iG213zIRmKQP?domain=microscopykarolinska.se" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/vt_4CNLwM9iG213zIRmKQP?domain=microscopykarolinska.se</a>>
<br/><br/> From: Arvydas Matiukas <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=7" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/> Sent: 16 October 2019 13:53
<br/> To: Sylvie Le Guyader <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=8" target="_top" rel="nofollow" link="external">[hidden email]</a>>; <a href="/user/SendEmail.jtp?type=node&node=7592356&i=9" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
<br/><br/> Hello Sylvie,
<br/><br/> Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.
<br/><br/> with best wishes Arvydas
<br/><br/><br/><br/><br/> +++++++++++++++++++++++++++++
<br/> Arvydas Matiukas, Ph.D.
<br/> Manager of NRB Shared Equipment
<br/> Director of Confocal&Two-Photon Core
<br/> SUNY Upstate Medical University
<br/><br/> >>> Sylvie Le Guyader <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=10" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592356&i=11" target="_top" rel="nofollow" link="external">[hidden email]</a>>> 10/16/19 6:28 AM >>>
<br/> *****
<br/> To join, leave or search the confocal microscopy listserv, go to:
<br/> <a href="https://protect-au.mimecast.com/s/k0SUCOMxNyty6oxMcP_F05?domain=urldefense.proofpoint.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/k0SUCOMxNyty6oxMcP_F05?domain=urldefense.proofpoint.com</a><br/> Post images on <a href="https://protect-au.mimecast.com/s/F4k8CP7yOZtk7qXPIrKiv7?domain=urldefense.proofpoint.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/F4k8CP7yOZtk7qXPIrKiv7?domain=urldefense.proofpoint.com</a> and include the link in your posting.
<br/> *****
<br/><br/> Hi Niyanta
<br/><br/><br/><br/> Alyona Minina has tons of videos on you tube. Check her channel<<a href="https://protect-au.mimecast.com/s/Dn7vCQnzP0ty5mPYc99xjB?domain=urldefense.proofpoint.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/Dn7vCQnzP0ty5mPYc99xjB?domain=urldefense.proofpoint.com</a> <<a href="https://protect-au.mimecast.com/s/dOszCROAQotzA8WxT05sni?domain=urldefense.proofpoint.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/dOszCROAQotzA8WxT05sni?domain=urldefense.proofpoint.com</a>> >.
<br/><br/><br/><br/> Med vänlig hälsning / Best regards
<br/><br/><br/><br/> Sylvie
<br/><br/><br/><br/> @@@@@@@@@@@@@@@@@@@@@@@@
<br/><br/> Sylvie Le Guyader, PhD
<br/><br/> Live Cell Imaging Facility Manager
<br/><br/> Karolinska Institutet- Bionut Dpt
<br/><br/> Blickagången 16,
<br/><br/> Room 7362 (lab)/7840 (office)
<br/><br/> 14157 Huddinge, Sweden
<br/><br/> mobile: +46 (0) 73 733 5008
<br/><br/> LCI website
<br/><br/> Follow our microscopy blog!
<br/><br/><br/><br/> -----Original Message-----
<br/> From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592356&i=12" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592356&i=13" target="_top" rel="nofollow" link="external">[hidden email]</a>>> On Behalf Of Niyanta Kumar
<br/> Sent: 11 October 2019 22:00
<br/> To: <a href="/user/SendEmail.jtp?type=node&node=7592356&i=14" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592356&i=15" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/> Subject: Confocal and Widefield microscopy training material
<br/><br/><br/><br/> *****
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<br/><br/> *****
<br/><br/><br/><br/> Hi all,
<br/><br/> Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.
<br/><br/> Thanks,
<br/><br/> Niyanta
<br/><br/><br/><br/> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<<a href="https://protect-au.mimecast.com/s/08zqCXLKZoizx3KWTx7TnX?domain=urldefense.proofpoint.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/08zqCXLKZoizx3KWTx7TnX?domain=urldefense.proofpoint.com</a> <<a href="https://protect-au.mimecast.com/s/XRVlCYWL1vi4zPlwurPdw1?domain=urldefense.proofpoint.com" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/XRVlCYWL1vi4zPlwurPdw1?domain=urldefense.proofpoint.com</a>> >.
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<br/><br/><br/><br/> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<<a href="https://protect-au.mimecast.com/s/2ZpGC2xZYvC9NA3gIWN0gM?domain=ki.se" target="_top" rel="nofollow" link="external">https://protect-au.mimecast.com/s/2ZpGC2xZYvC9NA3gIWN0gM?domain=ki.se</a>>.
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592355Re: MyScope is down. Any info?2021-06-14T05:18:52Z2021-06-14T05:18:52ZMichelle Peckham
*****
<br/>To join, leave or search the confocal microscopy listserv, go to:
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<br/>*****
<br/><br/>I found this in a previous email
<br/><br/>Introduction to MyScope – a free online microscopy training platform - Presented by Dr Jenny Whiting from Microscopy Australia (This will be recorded for future use)
<br/><br/>Is Jenny someone you might be able to contact?
<br/><br/>On 14/06/2021, 11:40, "Confocal Microscopy List on behalf of Aurora Panzera" <<a href="/user/SendEmail.jtp?type=node&node=7592355&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a> on behalf of <a href="/user/SendEmail.jtp?type=node&node=7592355&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> wrote:
<br/><br/> *****
<br/> To join, leave or search the confocal microscopy listserv, go to:
<br/> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/> *****
<br/><br/> Dear Microscopy people.
<br/><br/> The online learning environment MyScope <a href="https://myscope.training" target="_top" rel="nofollow" link="external">https://myscope.training</a> is down
<br/> since this morning.
<br/><br/> Does anyone have any news about whether the resource will be available
<br/> later today?
<br/><br/> We, Light Microscopy Facility of the Max Planck Tübingen, are currently
<br/> running a course for the master Students of Biochemistry:
<br/> The plan for this week is that students in their afternoon are
<br/> self-learning a certain Module from the MyScope which we facility-people
<br/> will then discuss and demonstrate on the day after.
<br/><br/> If this resource won't be available for the next day(s) we will need to
<br/> restructure our course.
<br/> I couldn't find an email address of the developers therefore I am writing
<br/> here to see if someone can give us some news.
<br/><br/> Otherwise, do you know if we can get some of the modules as a PDF(or other)
<br/> and how?
<br/><br/><br/> thanking you all
<br/><br/> Bests
<br/> Aurora
<br/><br/> --
<br/> Aurora Panzera (she/her)
<br/> Light Microscopy Facility
<br/> Max-Planck-Institute for Developmental Biology
<br/> Max-Planck-Ring 5
<br/> D-72076 Tuebingen
<br/> Germany
<br/><br/> Phone: +49 7071 601 1443
<br/> e-mail:<a href="/user/SendEmail.jtp?type=node&node=7592355&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/><br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592354Survey on Bioimaging Research Data Management2021-06-14T04:43:33Z2021-06-14T04:43:33ZChristian Schmidt
*****
<br/>To join, leave or search the confocal microscopy listserv, go to:
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<br/>*****
<br/><br/>Dear members of the Confocal Microscopy List,
<br/><br/>in Germany, the national research data infrastructure (Nationale
<br/>Forschungsdateninfrastruktur
<br/><<a href="https://www.dfg.de/en/research_funding/programmes/nfdi/index.html" target="_top" rel="nofollow" link="external">https://www.dfg.de/en/research_funding/programmes/nfdi/index.html</a>>,
<br/>NFDI) dedicated to research data management (RDM) is currently being
<br/>established. With a number of universties, Leibniz institutes, Helmholtz
<br/>centers, EMBL, incl. members from German BioImaging (GerBI-GMB) &
<br/>Euro-BioImaging & international partner initiatives (e.g., Open
<br/>Microscopy Environment OME, QUAREP-LiMi, and more), we have formed an
<br/>initiative, called NFDI4BIOIMAGE <<a href="https://nfdi4bioimage.de" target="_top" rel="nofollow" link="external">https://nfdi4bioimage.de</a>>, and we will
<br/>apply to the 3rd Call for NFDI consortia in September 2021.
<br/><br/>It is our central aim to serve the research community with respect to
<br/>its /RDM needs in bioimaging, including microscopy, biophotonics and
<br/>bioimage informatics/, with a primary focus on Germany, closely aligning
<br/>with international efforts.
<br/><br/>To help us assessing the current situation of RDM in bioimaging, we
<br/>kindly ask you to participate in our anonymous
<br/>*NFDI4BIOIMAGE Community Survey <<a href="https://nfdi4bioimage.de/en/survey" target="_top" rel="nofollow" link="external">https://nfdi4bioimage.de/en/survey</a>>.*
<br/><br/>We welcome contributions from all fields of research at all career
<br/>levels including research support areas.
<br/>We plan to share the survey results openly with the public.
<br/><br/>Please forward this E-Mail to interested colleagues and retweet our
<br/>announcement on Twitter
<br/><<a href="https://twitter.com/SchmChristian/status/1399664641480052741?s=20" target="_top" rel="nofollow" link="external">https://twitter.com/SchmChristian/status/1399664641480052741?s=20</a>>.
<br/><br/>Thank you very much for your contribution!
<br/><br/>Yours sincerely,
<br/><br/>Stefanie Weidtkamp-Peters (spokesperson),
<br/>Elisa May (co-spokesperson),
<br/>& Christian Schmidt (project coordinator)
<br/><br/>on behalf of the NFDI4BIOIMAGE initiative.
<br/>--
<br/>Dr. Christian Schmidt
<br/>/Coordinator NFDI4BIOIMAGE/
<br/>University of Konstanz
<br/>Bioimaging Center
<br/>Universitätsstraße 10
<br/>D-78457 Konstanz
<br/>phone: +49 (0) 7531 88-5337
<br/>mail to: <a href="/user/SendEmail.jtp?type=node&node=7592354&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592353MyScope is down. Any info?2021-06-14T03:29:25Z2021-06-14T03:29:25ZAurora Panzera
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<br/><br/> Dear Microscopy people.
<br/><br/>The online learning environment MyScope <a href="https://myscope.training" target="_top" rel="nofollow" link="external">https://myscope.training</a> is down
<br/>since this morning.
<br/><br/>Does anyone have any news about whether the resource will be available
<br/>later today?
<br/><br/>We, Light Microscopy Facility of the Max Planck Tübingen, are currently
<br/>running a course for the master Students of Biochemistry:
<br/>The plan for this week is that students in their afternoon are
<br/>self-learning a certain Module from the MyScope which we facility-people
<br/>will then discuss and demonstrate on the day after.
<br/><br/>If this resource won't be available for the next day(s) we will need to
<br/>restructure our course.
<br/>I couldn't find an email address of the developers therefore I am writing
<br/>here to see if someone can give us some news.
<br/><br/>Otherwise, do you know if we can get some of the modules as a PDF(or other)
<br/>and how?
<br/><br/><br/>thanking you all
<br/><br/>Bests
<br/>Aurora
<br/><br/>--
<br/>Aurora Panzera (she/her)
<br/>Light Microscopy Facility
<br/>Max-Planck-Institute for Developmental Biology
<br/>Max-Planck-Ring 5
<br/>D-72076 Tuebingen
<br/>Germany
<br/><br/>Phone: +49 7071 601 1443
<br/>e-mail:<a href="/user/SendEmail.jtp?type=node&node=7592353&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592352[Commercial Post] Invited webinar: Prof. Elisabeth Rhoades talks about "functional studies of dysfunctional proteins"2021-06-14T02:13:15Z2021-06-14T02:13:15ZAndré Devaux
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<br/>*****
<br/><br/>Dear list members,
<br/><br/>It is a great pleasure and honor to welcome Prof. Elizabeth Rhoades from
<br/>the University of Pennsylvania (USA) to our series of invited webinars.
<br/>On Friday June 25, 2021 at 10 am Eastern Daylight Time (EDT),
<br/>corresponding to 4 pm Berlin time (CEST), Prof. Elizabeth Rhoades will
<br/>share some insights into her work.
<br/><br/>What is the webinar about?
<br/>-------------------------------------
<br/><br/>Tau and alpha-synuclein are intrinsically disordered proteins,
<br/>abundantly expressed in the brain. Both are associated with the
<br/>pathology of devastating neurodegenerative diseases through their
<br/>abnormal aggregation into beta-sheet rich fibers. Despite intensive
<br/>study, many open questions remain about both the exact roles of these
<br/>proteins in disease development, as well as about their native
<br/>functions. We use fluorescence lifetime microscopy, single molecule
<br/>fluorescence and fluorescence correlation spectroscopy to characterize
<br/>the interactions of tau and alpha-synuclein with cellular binding
<br/>partners to provide insight into their functional mechanisms which, in
<br/>turn, may be used to identify novel therapeutic approaches to treat
<br/>disease.
<br/><br/>About the speaker
<br/>------------------------
<br/><br/>Prof. Elizabeth Rhoades obtained a B.S. in Physics and PhD in Biophysics
<br/>before joining the Group of Prof. Gilad Haran at the Weizmann Institute
<br/>as a postdoc , orking on single molecule FRET to study protein folding
<br/>dynamics. This was followed by a postdoctroal stay at the Cornell
<br/>University in the group of Prof. Watt Webb, where she employed FCS to
<br/>characterize protein-membrane interactions. She joined the Molecular
<br/>Biophysics & Biochemistry Department at Yale University in 2006, then
<br/>moved to the University of Pennsylvania in 2015. Her lab focuses on
<br/>functional mechanisms of intrinsically disordered proteins, often using
<br/>single molecule fluorescence and quantitative microscopy.
<br/><br/>Interested? Then register for free at: www.picoquant.com/webinars
<br/><br/>Best regards
<br/>André Devaux
<br/>PicoQuant GmbH
<br/><br/>postal address: Rudower Chaussee 29, 12489 Berlin, Germany
<br/>shipping address: Kekuléstraße 7, 12489 Berlin, Germany
<br/><br/>Tel: +49-30-1208820-644
<br/>Fax: +49-30-1208820-90
<br/>URL: <a href="http://www.picoquant.com" target="_top" rel="nofollow" link="external">http://www.picoquant.com</a><br/><br/>HRB 60901 Berlin Charlottenburg, Managing Director Rainer Erdmann
<br/>Privacy information according to Articles 13 and 14 GDPR can be found at
<br/><a href="https://www.picoquant.com/info_gdpr" target="_top" rel="nofollow" link="external">https://www.picoquant.com/info_gdpr</a><br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592351Re: Aperture Stop on a Nikon Ti-22021-06-09T19:46:07Z2021-06-09T19:46:07ZZdenek Svindrych-2
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<br/><br/>Hi Jeff,
<br/><br/>Short answer: 2 inch. Maybe more.
<br/><br/>Long answer: well, depends what your light source is. You've mentioned a
<br/>"lamp", but today it's most often a fiber or liquid light guide, sometimes
<br/>LED directly.
<br/>A "4-f" relay is easy to design, but it's never used in commercial systems,
<br/>as it's bulky, especially if you want both aperture and field stops. Adding
<br/>extra lenses makes the illumination setup more compact.
<br/>Generally, shorter focal length lenses can be smaller. But how close to
<br/>your objective lens do you think you can get? In commercial systems the
<br/>closest lens is just outside the filter turret housing, but in a homemade
<br/>setup with lenses outside the microscope body you're at 300 mm, if not
<br/>more. A simple ray trace on a piece of paper will give you an idea how big
<br/>the lenses (achromatic doublets) need to be for a given field of view (or
<br/>rather 'field number') and numerical aperture.
<br/>Also, lower power lenses tend to perform better optically than the short
<br/>focal length ones. Depending on your setup (Koehler vs 'critical') you
<br/>still may need a high-power collimating lens right at the light source (a
<br/>condenser lens).
<br/>Btw, I'd estimate the magnification between the lamp and the objective lens
<br/>around 5x for arc lamp setups.
<br/><br/>Best, zdenek
<br/><br/>On Wed, Jun 9, 2021 at 2:45 PM Jeff Spector <<a href="/user/SendEmail.jtp?type=node&node=7592351&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>> wrote:
<br/><div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Greetings,
<br/>> I'm working on setting up a system based around a Nikon Ti-2 body. For
<br/>> reasons out of my control the system does not have the normal Nikon Lapp
<br/>> adapters on the EPI port, but instead has sine non Nikon hardware attached
<br/>> to it. The custom hardware has a mirror and an open port where I can attach
<br/>> my lightsource. The issue is that I want to have ND filters, and more
<br/>> importantly and Aperture stop in the EPI light path ( like the stock Nikon
<br/>> hardware does). I think this can easily be accomplished with the use of a
<br/>> 4f lens system to relay the plane where I'd like the aperture to go (so we
<br/>> can control the incident NA of the epi lamp). My question is what size
<br/>> lenses to use,I think 1 or 2 inch lenses will work but I also think I need
<br/>> to be aware of the magnification. I'm not sure what is in the Nikon EPI
<br/>> arm, but I don't think it is a 1x mag between the lamp and the objective.
<br/>> If anybody knows this info, or has built their own "epi illuminator"
<br/>> please get back to me.
<br/>> Thanks!
<br/>> -Jeff
<br/>>
</div><br/><br/>--
<br/>--
<br/>Zdenek Svindrych, Ph.D.
<br/>Research Scientist - Microscopy Imaging Specialist
<br/>Department of Biochemistry and Cell Biology
<br/>Geisel School of Medicine at Dartmouth
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592350Job Opportunity: Postdoctoral Research Associate at King's College London UK2021-06-09T12:51:55Z2021-06-09T12:51:55ZBullen, Anwen
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<br/>*****
<br/><br/>Dear all,
<br/><br/>We have a job opportunity for a Post-Doctoral Research Associate. The post is fixed-term for 36 months and is based at King's College London in the lab of Dr Zoe Mann. The project would be carried out in collaboration with myself and Dr Dan Jagger at the UCL Ear Institute.
<br/><br/>The aim of the project is to use a combination of microscopy techniques, confocal microscopy, FLIM, and volume electron microscopy to examine the role of metabolism and mitochondrial dynamics in the development of the auditory system. We are looking for a motivated post-doc wth an interest in microscopy and cell and developmental biology.
<br/><br/>Job Posting: <a href="https://jobs.kcl.ac.uk/gb/en/job/024686/Post-doctoral-Research-Associate-in-Developmental-Biology-and-Cell-Metabolism" target="_top" rel="nofollow" link="external">https://jobs.kcl.ac.uk/gb/en/job/024686/Post-doctoral-Research-Associate-in-Developmental-Biology-and-Cell-Metabolism</a><br/><br/>For any enquiries, please contact Dr Zoe Mann (<a href="/user/SendEmail.jtp?type=node&node=7592350&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>)
<br/><br/>Best,
<br/><br/>Anwen
<br/><br/><br/>Dr Anwen Bullen
<br/>UCL Ear Institute
<br/>332 Gray's Inn Road
<br/>London
<br/>WC1X 8EE
<br/>E-mail: <a href="/user/SendEmail.jtp?type=node&node=7592350&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592349Re: Aperture Stop on a Nikon Ti-22021-06-09T11:51:30Z2021-06-09T11:51:30ZCraig Brideau
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<br/>*****
<br/><br/>Hi Jeff, I've tried this myself with a Ti-1 and I found you can get
<br/>considerable chromatic aberration depending on the lenses you use. If you
<br/>are working with filtered light with narrow bandwidth you will be OK, but
<br/>if you are working with polychromatic light or several widely-spaced colors
<br/>it can be problematic. I've actually used Nikon Tube Lenses as relay lenses
<br/>and it was... ok for narrow bandwidth light but terrible for poly.
<br/><br/>Craig
<br/><br/>On Wed, Jun 9, 2021 at 12:45 PM Jeff Spector <<a href="/user/SendEmail.jtp?type=node&node=7592349&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>> wrote:
<br/><div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Greetings,
<br/>> I'm working on setting up a system based around a Nikon Ti-2 body. For
<br/>> reasons out of my control the system does not have the normal Nikon Lapp
<br/>> adapters on the EPI port, but instead has sine non Nikon hardware attached
<br/>> to it. The custom hardware has a mirror and an open port where I can attach
<br/>> my lightsource. The issue is that I want to have ND filters, and more
<br/>> importantly and Aperture stop in the EPI light path ( like the stock Nikon
<br/>> hardware does). I think this can easily be accomplished with the use of a
<br/>> 4f lens system to relay the plane where I'd like the aperture to go (so we
<br/>> can control the incident NA of the epi lamp). My question is what size
<br/>> lenses to use,I think 1 or 2 inch lenses will work but I also think I need
<br/>> to be aware of the magnification. I'm not sure what is in the Nikon EPI
<br/>> arm, but I don't think it is a 1x mag between the lamp and the objective.
<br/>> If anybody knows this info, or has built their own "epi illuminator"
<br/>> please get back to me.
<br/>> Thanks!
<br/>> -Jeff
<br/>>
<br/></div>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592348Aperture Stop on a Nikon Ti-22021-06-09T11:44:41Z2021-06-09T11:44:41ZJeff Spector
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<br/><a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>*****
<br/><br/>Greetings,
<br/> I'm working on setting up a system based around a Nikon Ti-2 body. For
<br/>reasons out of my control the system does not have the normal Nikon Lapp
<br/>adapters on the EPI port, but instead has sine non Nikon hardware attached
<br/>to it. The custom hardware has a mirror and an open port where I can attach
<br/>my lightsource. The issue is that I want to have ND filters, and more
<br/>importantly and Aperture stop in the EPI light path ( like the stock Nikon
<br/>hardware does). I think this can easily be accomplished with the use of a
<br/>4f lens system to relay the plane where I'd like the aperture to go (so we
<br/>can control the incident NA of the epi lamp). My question is what size
<br/>lenses to use,I think 1 or 2 inch lenses will work but I also think I need
<br/>to be aware of the magnification. I'm not sure what is in the Nikon EPI
<br/>arm, but I don't think it is a 1x mag between the lamp and the objective.
<br/>If anybody knows this info, or has built their own "epi illuminator"
<br/>please get back to me.
<br/>Thanks!
<br/>-Jeff
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592347Re: Inquire about Fees for confocal High Content Screening2021-06-09T06:55:33Z2021-06-09T06:55:33ZChloe van Oostende
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<br/><a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>*****
<br/><br/>Hi Claire,
<br/>No high-content confocal at uOttawa either.
<br/>However, Incucyte is 1$/h per slot (we can put 6 multi-well plates). This
<br/>is for long-term live imaging.
<br/>For other live imaging and screening: 20$/h.
<br/>Take care
<br/>Chloe
<br/><br/><br/>*Chloë van Oostende, PhD*
<br/><br/>*Cell Biology and Image Acquisition Core Facility manager*
<br/>*Senior Microscopy specialist *
<br/>Faculty of Medicine
<br/>University of Ottawa
<br/><br/>451 Smyth Road
<br/>Ottawa, ON K1H 8M5
<br/>Office: 613-562-5800 ext: 8376
<br/><br/><a href="http://www.chloevanoostende.net" target="_top" rel="nofollow" link="external">http://www.chloevanoostende.net</a><br/>CBIA core facility
<br/><<a href="http://www.med.uottawa.ca/research/corelabs/CoreLabs_CBIA/eng/" target="_top" rel="nofollow" link="external">http://www.med.uottawa.ca/research/corelabs/CoreLabs_CBIA/eng/</a>>
<br/><br/><br/>Le mar. 11 mai 2021 à 13:45, Claire Brown, Dr. <<a href="/user/SendEmail.jtp?type=node&node=7592347&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>> a
<br/>écrit :
<br/><div class='shrinkable-quote'><br/>> *****
<br/>> To join, leave or search the confocal microscopy listserv, go to:
<br/>> <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>> Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
<br/>> *****
<br/>>
<br/>> Hello,
<br/>>
<br/>> We are reviewing our fee structure for confocal based high content
<br/>> screening.
<br/>> Can anyone share their rates with me? Feel free to email me directly if
<br/>> you prefer or send a link to your facility fees.
<br/>>
<br/>> We have the Perkin Elmer Opera Phenix.
<br/>>
<br/>> All the best,
<br/>>
<br/>> Claire
<br/>>
<br/>>
<br/>> Claire M. Brown, PhD - McGill University
<br/>>
<br/></div>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592346Re: nuclear marking fluorophores - for simpler image analysis2021-06-08T12:32:30Z2021-06-08T12:32:30ZSylvie Le Guyader
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<br/>*****
<br/><br/>Hi Jeremy
<br/><br/>We like to use laminA antibodies. Robust staining and easy to segment. :)
<br/><br/>Med vänlig hälsning / Best regards
<br/>Sylvie
<br/>@@@@@@@@@@@@@@@@@@@@@@@@
<br/>Sylvie Le Guyader, PhD
<br/>Live Cell Imaging Facility Manager
<br/>Karolinska Institutet- Bionut Dpt
<br/>Blickagången 16,
<br/>Room 7362 (lab)/7840 (office)
<br/>14157 Huddinge, Sweden
<br/>mobile: +46 (0) 73 733 5008
<br/>LCI website
<br/>LCI microscopy blog
<br/><br/>-----Original Message-----
<br/>From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592346&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>> On Behalf Of Jeremy Adler
<br/>Sent: Wednesday, 2 June, 2021 12:32
<br/>To: <a href="/user/SendEmail.jtp?type=node&node=7592346&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>Subject: nuclear marking fluorophores - for simpler image analysis
<br/><br/>*****
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<br/>*****
<br/><br/>Dapi and similar fluorophores are regularly used to highlight nuclei.
<br/>But the staining is inhomogenous within nuclei and can vary substantially between nuclei in the same specimen, which makes segmentation for quantitative analysis difficult.
<br/>So any suggestions for a marker that produces a more uniform fluorescence within individual nuclei and less variation between nuclei, which would make image analysis simpler when we only want to know where the nuclei are.
<br/><br/>Jeremy Adler
<br/>BioVis
<br/>Uppsala U
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592345Postdoctoral Opportunities in Quantitative Imaging at NIST2021-06-08T10:26:55Z2021-06-08T10:26:55ZMichael Halter-2
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<br/><br/>Postdoctoral opportunities are available in the Biosystems and Biomaterials Division (<a href="https://www.nist.gov/people/anne-l-plant" target="_top" rel="nofollow" link="external">https://www.nist.gov/people/anne-l-plant</a> and <a href="https://www.nist.gov/people/michael-halter" target="_top" rel="nofollow" link="external">https://www.nist.gov/people/michael-halter</a>) at the National Institute of Standards and Technology.
<br/><br/>Our efforts focus on quantitative imaging of cellular dynamics with the goal of improving measurement methods and reliability for the development, manufacturing and deployment of cell-based therapies.
<br/><br/>To carry this out, we are advancing the rate and volume with which image data can be acquired, processed, and analyzed. This work involves instrument development, computational methods for large biological image datasets, deep learning methods for dynamic analysis of living cells, massive integration of data and analysis, and AI validation. Computational work is performed in collaboration with the Software and Systems Division at NIST (<a href="https://isg.nist.gov/deepzoomweb/" target="_top" rel="nofollow" link="external">https://isg.nist.gov/deepzoomweb/</a> and <a href="https://www.nist.gov/itl/ssd/information-systems-group" target="_top" rel="nofollow" link="external">https://www.nist.gov/itl/ssd/information-systems-group</a>).
<br/>
<br/>In the Biosystems and Biomaterials Division we are interested in collecting data from large numbers of single cells to characterize population phenotypes and understand their dynamics. We are examining how dynamic imaging of engineered fluorescent reporter cell lines enables the modeling of gene regulatory networks by statistical thermodynamics ( <a href="https://doi.org/10.1073/pnas.1207544109;" target="_top" rel="nofollow" link="external">https://doi.org/10.1073/pnas.1207544109;</a> <a href="https://doi.org/10.1371/journal.pone.0230076;" target="_top" rel="nofollow" link="external">https://doi.org/10.1371/journal.pone.0230076;</a> <a href="https://doi.org/10.3390/e23010063;" target="_top" rel="nofollow" link="external">https://doi.org/10.3390/e23010063;</a> <a href="https://doi.org/10.1016/j.csbj.2020.09.025" target="_top" rel="nofollow" link="external">https://doi.org/10.1016/j.csbj.2020.09.025</a> ). We employ iPSCs and other cell types of interest to regenerative medicine and cellular engineering.
<br/><br/>Candidates should have experience in one or more of the following areas: live cell microscopy, systems biology, software engineering, AI computational methods, and theoretical modeling.
<br/><br/>Interested applicants can contact Michael Halter and/or Anne Plant (information below):
<br/><br/>Michael Halter
<br/><a href="/user/SendEmail.jtp?type=node&node=7592345&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/><br/>Anne Plant
<br/><a href="/user/SendEmail.jtp?type=node&node=7592345&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592344Commercial Post: Scientifica Are Recruiting!2021-06-08T08:29:50Z2021-06-08T08:29:50ZPhillipa Timmins
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<br/><br/>Dear All,
<br/><br/>I have recently joined Scientifica as Sales Manager for the EMEIA territory. I am pleased to announce that we are recruiting for a Product Specialist to join our sales team, based in the UK.
<br/><br/>This person would be responsible for selling the Scientifica multiphoton and electrophysiology products as part of our dynamic and supportive sales team. The role is home based with benefits including, but not limited to:
<br/><br/>Flexible working
<br/>Employee discount scheme
<br/>Life assurance/death in service
<br/>Long term income protection
<br/>Wellbeing programme
<br/>Judges Scientific PLC Share Incentive Plan
<br/><br/>If you are a self starter, having some experience selling capital or scientific equipment, educated to degree level in a life science discipline and have an interest in neuroscience we would love to hear from you.
<br/><br/>If you would like to receive more information about this role, please feel free to drop me an email or contact <a href="/user/SendEmail.jtp?type=node&node=7592344&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592344&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>>
<br/><br/>All the best!
<br/><br/>Phillipa
<br/><br/>Dr Phillipa Timmins / Sales Manager EMEIA
<br/><a href="/user/SendEmail.jtp?type=node&node=7592344&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a> / +44 (0)7392 873218
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592343Re: Advice on high-end light-sheet2021-06-08T06:11:23Z2021-06-08T06:11:23ZSylvie Le Guyader
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<br/><br/>Hi Javier
<br/><br/>We have an Aurora light sheet from MSquared Lasers and are delighted with it. It is very versatile and with its Airy beam, offers high res across a large (around 800 um) FOV. :)
<br/><br/>Med vänlig hälsning / Best regards
<br/><br/>Sylvie
<br/><br/>@@@@@@@@@@@@@@@@@@@@@@@@
<br/>Sylvie Le Guyader, PhD
<br/>Live Cell Imaging Facility Manager
<br/><br/>Karolinska Institutet- Bionut
<br/>Hälsovägen 7C,
<br/>Room 7362 (lab)/7840 (office)
<br/>14157 Huddinge, Sweden
<br/>mobile: +46 (0) 73 733 5008
<br/>LCI website
<br/>Sign in to follow our microscopy blog!
<br/><br/>-----Original Message-----
<br/>From: Confocal Microscopy List <<a href="/user/SendEmail.jtp?type=node&node=7592343&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>> On Behalf Of F Javier Díez Guerra
<br/>Sent: 02 June 2021 20:15
<br/>To: <a href="/user/SendEmail.jtp?type=node&node=7592343&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>Subject: Advice on high-end light-sheet
<br/><br/>*****
<br/>To join, leave or search the confocal microscopy listserv, go to:
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<br/><br/>Hi,
<br/><br/>We are considering several options for a high-end light-sheet (lattice) system and would like to know your experience. The system is meant for our advanced light microscopy facility at the CBMSO in Madrid, with many users and projects from different fields of the biosciences. So we value versatility and robustness, in addition to specifications.
<br/><br/>I would be very grateful to listen to your comments off-list (if you think it should be that way).
<br/><br/>Thanks all for your cooperation.
<br/><br/>Best
<br/><br/>Javier
<br/><br/><br/>F Javier Diez-Guerra, PhD
<br/>Servicio de Microscopía Confocal
<br/>Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Campus de Cantoblanco
<br/>28049 Madrid
<br/>SPAIN
<br/><br/>Tel +34 91 196 4612
<br/>e-mail: <a href="/user/SendEmail.jtp?type=node&node=7592343&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/><br/><br/>När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<<a href="https://ki.se/medarbetare/integritetsskyddspolicy" target="_top" rel="nofollow" link="external">https://ki.se/medarbetare/integritetsskyddspolicy</a>>.
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592342Image analysis and datsa processing workshop 20212021-06-08T02:20:47Z2021-06-08T02:20:47ZZuzana Burdikova
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<br/><br/>Dear Colleagues,
<br/><br/>we would like to invite you to the *Image analysis and data processing in
<br/>superresolution microscopy 2021* workshop.
<br/><br/>*The workshop will take place at Charles University in Prague from Monday,
<br/>August 30th to Wednesday, September 1st, 2021. *
<br/>We are optimistic that the coronavirus situation will allow an in-person
<br/>meeting, but we will prepare the online version if necessary as well.
<br/><br/>The workshop's main purpose is to provide a stimulating environment for the
<br/>creation of advanced image analysis pipelines in
<br/>superresolution microscopy.
<br/><br/>*Detailed program *and enrollment are available here
<br/><<a href="https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.google.com%2Fdocument%2Fd%2F10rzXsIQfZeoJlbh-eJn8PlOaYYhtdVEl%2Fedit%23heading%3Dh.ihe6w9yzsvvs&data=04%7C01%7Czdenek.svindrych%40dartmouth.edu%7Cb8e216f4cf3f414bb2c808d929c28571%7C995b093648d640e5a31ebf689ec9446f%7C0%7C1%7C637586737827499561%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=E5Tx6LFO183THHpETm6i%2BiDhgtX6T8NM1to4eQWRgJs%3D&reserved=0" target="_top" rel="nofollow" link="external">https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.google.com%2Fdocument%2Fd%2F10rzXsIQfZeoJlbh-eJn8PlOaYYhtdVEl%2Fedit%23heading%3Dh.ihe6w9yzsvvs&data=04%7C01%7Czdenek.svindrych%40dartmouth.edu%7Cb8e216f4cf3f414bb2c808d929c28571%7C995b093648d640e5a31ebf689ec9446f%7C0%7C1%7C637586737827499561%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=E5Tx6LFO183THHpETm6i%2BiDhgtX6T8NM1to4eQWRgJs%3D&reserved=0</a>>
<br/>.
<br/><br/>*The registration is open here:*
<br/><br/><a href="https://forms.gle/F9xRRzeg6SjkQZef8" target="_top" rel="nofollow" link="external">https://forms.gle/F9xRRzeg6SjkQZef8</a><br/><br/>We are looking forward to seeing you!
<br/><br/>Kind regards
<br/>Your KONFMI team
<br/><br/>Zuzana Burdikova, Martin Schätz, Peter Hoboth and Ondřej Šebesta
<br/><br/><br/>Zuzana Burdíková, Ph.D
<br/><br/>Laboratory of Confocal and Fluorescence Microscopy
<br/>Charles University in Prague, Faculty of Science
<br/>Albertov 6, 128 43 Praha 2, Czech Republic
<br/><br/>Tel.: +420 221951061
<br/>Cell: +420 775305602
<br/>E-mail: <a href="/user/SendEmail.jtp?type=node&node=7592342&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592341****Commercial Post**** MATRIX: STED reloaded (Online symposium)2021-06-08T00:53:12Z2021-06-08T00:53:12ZMatthias Reuss
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<br/><br/>Dear Community,
<br/><br/>Tomorrow, abberior will host another online symposium:
<br/><br/> June 9th, 15:30 CEST (9:30 ET).
<br/> Register here: register.matrix-sted.com
<br/><br/>Join us and Dr. Martin Meschkat to learn more about the technology behind MATRIX detection, superresolution imaging of thick samples, and how to remove background and increase optical sectioning.
<br/><br/>We are looking forward to seeing you!
<br/><br/>Kind regards
<br/>Your abberior team
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592340Job Opportunity in Ottawa- Canada2021-06-07T14:24:39Z2021-06-07T14:24:39ZChloe van Oostende
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<br/><a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" target="_top" rel="nofollow" link="external">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><br/>Post images on <a href="http://www.imgur.com" target="_top" rel="nofollow" link="external">http://www.imgur.com</a> and include the link in your posting.
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<br/><br/>Hi everyone!
<br/>I would appreciate if you can pass this job to anybody who might be
<br/>interested!
<br/><br/>Thank you!
<br/><br/>Take care!
<br/><br/>Chloe
<br/><br/><br/>*Job Title: Light Microscopy Imaging Specialist*
<br/><br/><br/><br/>We are seeking a highly skilled imaging specialist for the Cell Biology and
<br/>Imaging Analysis (CBIA) core facility at the Faculty of Medicine of
<br/>University of Ottawa. Our facility provides access to advanced imaging
<br/>techniques such as live, high- and super-resolution imaging as well as
<br/>high-throughput microscopy. The CBIA core offers support from sample
<br/>preparation to image analysis and is accessed by a broad community
<br/>including cell biologists, neuroscientists, virologists, pathologists, and
<br/>engineers. The primary responsibilities of the successful candidate will be
<br/>to work with the facility manager to provide extensive training and
<br/>advanced technical assistance to users of the facility as well as maintain
<br/>the instrumentation and its associated infrastructure.
<br/><br/><br/><br/>It is expected that the successful candidate will design, apply and
<br/>interpret a variety of complex microscopy experiments from image
<br/>acquisition to image analysis; will continue to improve and expand their
<br/>expertise to develop more advanced imaging protocols across a variety of
<br/>platforms (Zeiss, Leica, Quorum, and Deltavision); and will continue to
<br/>expand their skills in image analysis.
<br/><br/><br/><br/>*Responsibilities*:
<br/><br/>1) User training (entry level to advanced)
<br/><br/>2) Image analysis support (Imaris, Fiji, create macros with
<br/>CellProfiler and Matlab,)
<br/><br/>3) Routine and meticulous maintenance of instruments
<br/><br/>4) Work with manager to monitor equipment access
<br/><br/>5) Maintain and update CBIA web page
<br/><br/>6) Participate in the development of outreach activities and core
<br/>promotion
<br/><br/><br/><br/><br/><br/>*Qualifications*:
<br/><br/> 1. Graduate level training (PhD preferred or equivalent experience) with
<br/> solid background in light microscopy.
<br/> 2. Extensive experience with epifluorescence and confocal microscopy.
<br/> 3. Extensive experience with immunostaining.
<br/> 4. High level experience with Image Analysis (Fiji, Imaris, Matlab)
<br/> 5. Ability to learn new and demanding techniques.
<br/> 6. Adaptable and detail-oriented
<br/> 7. Coding in Python and Matlab will be an asset.
<br/><br/><br/><br/>Salary will be commensurate with experience and will include access to the
<br/>University of Ottawa benefit and pension plans*
<br/><br/><br/><br/>To apply, submit by e-mail a cover letter and CV highlighting your relevant
<br/>experience to <a href="/user/SendEmail.jtp?type=node&node=7592340&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>.
<br/><br/>*Deadline*: July 23rd, 2021
<br/><br/>Informal inquiries can also be directed by e-mail to Dr. Copeland, director
<br/>of the CBIA
<br/><br/>*pursuant eligibility requirements
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592339DiasproLab - PhD calls in Genoa, Italy2021-06-05T13:12:33Z2021-06-05T13:12:33ZAlberto Diaspro-2
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<br/><br/>Dear,
<br/>In Genoa we have 4 new PhD calls.
<br/>All the best
<br/>Alby
<br/><br/><br/>——————————————————————————
<br/><br/>Do not miss the New PhD Call – 37th cycle at DIASPROLAB.
<br/><br/>PhD programs at UNIGE (1, 2, 3): <a href="https://unige.it/en/usg/en/phd-programmes" target="_top" rel="nofollow" link="external">https://unige.it/en/usg/en/phd-programmes</a> <<a href="https://unige.it/en/usg/en/phd-programmes" target="_top" rel="nofollow" link="external">https://unige.it/en/usg/en/phd-programmes</a>>
<br/>Deadline, June 15th 2021, 12 p.m. CEST
<br/><br/>PhD program at UNIPARMA (4): <a href="https://en.unipr.it/studying/research-doctorates" target="_top" rel="nofollow" link="external">https://en.unipr.it/studying/research-doctorates</a> <<a href="https://en.unipr.it/studying/research-doctorates" target="_top" rel="nofollow" link="external">https://en.unipr.it/studying/research-doctorates</a>>
<br/>Development of a Multimodal Optical Microscopy Image Correlation Sensing - MOMIX™ - a super resolution microscope to study cellular systems at the nanoscale.
<br/><br/>Investigating life at the cellular and molecular level demands for super-resolution (<200nm) imaging techniques capable of providing three-dimensional access to structural and functional information. The MOMIX™ research project deals with the development of an innovative super-resolution optical microscope capable of combining spatial super-resolution (e.g. confocal, image scanning and STED microscopy) with spectroscopic approaches like fluorescence lifetime and circular intensity differential scattering, CIDS. Successful implementation of the MOMIX™ microscope will result in an innovative quantitative imaging approach in nanoscale biophysics and bioengineering.
<br/><br/>The ideal candidate holds a Master Degree in a technical or scientific field (e.g. Physics, Engineering, Computer Science). Knowledge in scientific programming with Matlab, Labview or Python is desirable.
<br/><br/>Applicants will be part of an international group located at Center of Human Technologies, Great Campus Erzelli - IIT and Department of Physics (DIFI), University of Genoa.
<br/>
<br/>The successful candidate will have the chance to participate in national and international training opportunities, workshops, and to access funding for visits abroad.
<br/><br/>Research team: Alberto Diaspro, (supervisor, DIFI-UNIGE, IIT), Paolo Bianchini (DIFI-UNIGE. IIT), Marco Castello (IIT), Simonluca Piazza (IIT), Irene Nepita (IIT).
<br/><br/>Bib ref (open access): Diaspro, A., Bianchini, P. Optical nanoscopy. Riv. Nuovo Cim. 43, 385–455 (2020). <a href="https://doi.org/10.1007/s40766-020-00008-1" target="_top" rel="nofollow" link="external">https://doi.org/10.1007/s40766-020-00008-1</a> <<a href="https://doi.org/10.1007/s40766-020-00008-1" target="_top" rel="nofollow" link="external">https://doi.org/10.1007/s40766-020-00008-1</a>>
<br/>For more information please contact <a href="/user/SendEmail.jtp?type=node&node=7592339&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a> <mailto:<a href="/user/SendEmail.jtp?type=node&node=7592339&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> with subject ‘PhD-2021-MOMIX’
<br/><br/>2. Label-free optical microscopy, using circular intensity differential scattering (CIDS) and non linear processes (multiphoton and second harmonic generation), towards nanoscale biophysics applications.
<br/><br/>The project deals with the realization of label free optical modules, incorporating advanced linear and non-linear fluorescence approaches, based on phase-contrast, circular intensity differential scattering (CIDS) and second-harmonic generated signals. The system will benefit of advances in Mueller matrix microscopy and multiphoton imaging. Case studies will be related to exploit in a light-matter interaction context, ray tracing and photon detection, light matters interactions in single cells, cellular spheroids and tissues.
<br/><br/>The ideal candidate holds a Master Degree in a technical or scientific field (e.g. Physics, Engineering, Computer Science). Knowledge in scientific programming with Matlab, Labview or Python is desirable.
<br/><br/><br/>Applicants will be part of an international group located at Center of Human Technologies, Great Campus Erzelli - IIT and Department of Physics (DIFI), University of Genoa.
<br/>
<br/>The successful candidate will have the chance to participate in national and international training opportunities, workshops, and to access funding for visits abroad.
<br/><br/>Research team: Alberto Diaspro, (supervisor, DIFI-UNIGE, IIT) Paolo Bianchini, (DIFI-UNIGE,IIT) Irene Nepita (IIT).
<br/><br/>Bib ref (open access): Diaspro, A., Bianchini, P. Optical nanoscopy. Riv. Nuovo Cim. 43, 385–455 (2020). <a href="https://doi.org/10.1007/s40766-020-00008-1" target="_top" rel="nofollow" link="external">https://doi.org/10.1007/s40766-020-00008-1</a> <<a href="https://doi.org/10.1007/s40766-020-00008-1" target="_top" rel="nofollow" link="external">https://doi.org/10.1007/s40766-020-00008-1</a>>.
<br/>For more information please contact <a href="/user/SendEmail.jtp?type=node&node=7592339&i=2" target="_top" rel="nofollow" link="external">[hidden email]</a> <mailto:<a href="/user/SendEmail.jtp?type=node&node=7592339&i=3" target="_top" rel="nofollow" link="external">[hidden email]</a>> with subject ‘PhD-2021-LFREE’
<br/><br/>3. Multimodal optical nanoscopy to study chromatin organization during cell differentiation and neoplastic transformation.
<br/><br/>Nuclear architecture and chromatin remodelling are the central regulators of cell identity and fate. Today we can follow chromatin organization mechanisms at the molecular level, taking advantage of a multimodal optical approach as the one provided by super-resolved optical fluorescence microscopy coupled with label-free CIDS (circular intensity differential scattering signature). The project focuses on neuroblastoma (NB), the most common extracranial solid tumour in childhood. Since the molecular and genetic mechanisms involved in NB are still partially unknown, we aim characterizing changes of chromatin nanoscale architecture correlated with NB transformation. The research will integrate multimodal optical data towards the outcome of unveiling whether NB-associated chromatin alteration locates in correspondence of specific territories or genes and paving the way towards new prognostic and therapeutic approaches.
<br/><br/>The ideal candidate holds a preferred degree on Molecular and cellular Biology, Biotechnology, Physics (Biophysics), Bioengineering.
<br/><br/>Applicants will be part of an international group located at Center of Human Technologies, Great Campus Erzelli - IIT and Department of Earth Sciences, of the Environment and of Life Sciences (DISTAV), University of Genoa.
<br/><br/>The successful candidate will have the chance to participate in national and international training opportunities, workshops, and to access funding for visits abroad.
<br/><br/>Project supervised and tutored by Laura Vergani (DISTAV-UNIGE), Alberto Diaspro (IIT, DIFI-UNIGE), Francesca Baldini (IIT).
<br/><br/><br/>4. Biophysics of Chromatin by Correlative Imaging and Simulation.
<br/><br/>Nucleosomes and chromatin regulate the accessibility to the genome and thereby mediate and control DNA processes, including transcription, replication, and repair.
<br/>Atomic force microscopy (AFM) and electron microscopy (EM) are excellent candidates for the investigation of mechanical and structural properties of the nucleus, while lacking of chemical specificity. Thus, optical advanced and super-resolution microscopy, with its high specificity, can complement collecting dynamic information at molecular level in living samples.
<br/>We aim developing a work flow that enables a multimodal and correlative approach. For instance, the use of functionalised photosensitiser molecules can enable super-resolution optical nanoscopy, but also generating the condition for diaminobenzidine (DAB) oxidation and precipitation, would enable correlative light-electron microscopy (CLEM).
<br/><br/>Applicants will be part of an international group located at Center of Human Technologies, Great Campus Erzelli - IIT and Department of Mathematical, Physical and Computer Sciences
<br/>University of PARMA.
<br/><br/>The successful candidate will have the chance to participate in national and international training opportunities, workshops, and to access funding for visits abroad.
<br/><br/>Project supervised and tutored by Paolo Bianchini (IIT), Cristiano Viappiani (UNIPR), Alberto Diaspro (IIT, DIFI-UNIGE).
<br/>Some refs: Bendandi, A., S. Dante, S.R. Zia, A. Diaspro, and W. Rocchia. 2020. Chromatin Compaction Multiscale Modeling: A Complex Synergy Between Theory, Simulation, and Experiment. Frontiers Mol Biosci. 7:15. Cosentino, M., C. Canale, P. Bianchini, and A. Diaspro. 2019. AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging. Science Advances. 5:eaav8062. Bianchini, P., M. Cozzolino, M. Oneto, L. Pesce, F. Pennacchietti, M. Tognolini, C. Giorgio, S. Nonell, L. Cavanna, P. Delcanale, S. Abbruzzetti, A. Diaspro, and C. Viappiani. 2019. Hypericin-Apomyoglobin: An Enhanced Photosensitizer Complex for the Treatment of Tumor Cells. Biomacromolecules. 20:2024-2033.
<br/><br/>------------------------------------------------------
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592338Re: Job Posting at Rutgers NJMS Imaging Core2021-06-04T08:26:40Z2021-06-04T08:26:40ZLuke Fritzky
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<br/><br/>The position for a Microscopic Imaging Specialist at Rutgers RBHS Newark NJ has been reposted. Please see the information and the new link below.
<br/><br/>Rutgers, The State University of New Jersey, is seeking a Microscopic Imaging Specialist for the Imaging Core Facility in the Office of Research at New Jersey Medical School. Under the direction of the facility manger the Microscopic Imaging Specialist will be required to operate and maintain the high-end microscopic imaging systems within the Core Facility, including the Nikon A1R HD SI confocal microscope, the Nikon Ti2 High Content Imaging System, the Objective Imaging whole slide imaging system, the Zeiss PALM laser capture microdissection microscope, the brand new Leica Stellaris 8 tau-STED 3x Nanoscopy system, as well as other microscope systems with-in the core. Will be directly responsible for the training and assistance of researchers using the microscopy systems within the core facility.
<br/><br/>Full Job Posting: <a href="https://jobs.rutgers.edu/postings/133351" target="_top" rel="nofollow" link="external">https://jobs.rutgers.edu/postings/133351</a><br/><br/>Job Posting Contact: Luke Fritzky, <a href="/user/SendEmail.jtp?type=node&node=7592338&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/><br/>Thank you,
<br/>Luke Fritzky
<br/><br/>Cellular Imaging and Histology Core
<br/>NJMS Cancer Center
<br/>205 South Orange Ave.
<br/>Newark, NJ 07103
<br/>Phone- (973) 972-8101
<br/>Cell Phone (973) 907-3714
<br/>Email- <a href="/user/SendEmail.jtp?type=node&node=7592338&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592337Job offer: Scientist for the imaging facility at the MPI for Brain Research, Frankfurt2021-06-04T06:40:04Z2021-06-04T06:40:04ZStephan Junek
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<br/><br/>Dear all,
<br/><br/>we recently posted a job offer for our imaging facility. Our work is
<br/>very varied, and includes method development and building of
<br/>microscopes. You can take a look at our homepage here:
<br/><a href="https://brain.mpg.de/imaging" target="_top" rel="nofollow" link="external">https://brain.mpg.de/imaging</a><br/><br/>The job offer can be found here:
<br/><a href="https://jobs.brain.mpg.de/jobposting/578d4992261783df039b8516332beeec44f9497f0?ref=homepage" target="_top" rel="nofollow" link="external">https://jobs.brain.mpg.de/jobposting/578d4992261783df039b8516332beeec44f9497f0?ref=homepage</a>
<br/><br/><br/>I would be grateful if you could forward this mail to anyone you think
<br/>might be interested.
<br/><br/>Best wishes,
<br/><br/>Stephan
<br/><br/>--
<br/>________________________________________
<br/><br/>Dr. Stephan Junek
<br/>Head of the imaging facility
<br/>Max Planck Institute for Brain Research
<br/>Max-von-Laue-Str. 4
<br/>60438 Frankfurt am Main
<br/>Germany
<br/><br/><a href="/user/SendEmail.jtp?type=node&node=7592337&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a>
<br/>T: +49 69 850033 – 2531 (Office)
<br/>T: +49 69 850033 – 1409 (Lab)
<br/>F: +49 69 850033 – 2102
<br/>
tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592336Reminder: Microscopy Position at ICR London2021-06-04T02:12:24Z2021-06-04T02:12:24ZKai Betteridge
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<br/><br/>Dear All
<br/><br/><br/><br/>Just a reminder that there is a job opportunity within the core microscopy facility at the Institute of Cancer Research (ICR) in Chelsea, London for the role of Microscopist and Image Analyst. The closing date for applications is this Sunday (06th of June).
<br/><br/><br/><br/>Details of the post can be found using the following link:
<br/><br/><br/><br/><a href="https://icr.tal.net/vx/appcentre-ext/brand-0/candidate/so/pm/1/pl/1/opp/1285-Microscopist-Image-Analyst/en-GB" target="_top" rel="nofollow" link="external">https://icr.tal.net/vx/appcentre-ext/brand-0/candidate/so/pm/1/pl/1/opp/1285-Microscopist-Image-Analyst/en-GB</a><br/><br/><br/><br/>Please share widely with anyone who you think might have an interest.
<br/><br/><br/><br/>With Kind Regards
<br/><br/><br/><br/>Kai
<br/><br/>Dr Kai Betteridge | Light Microscopy Facility Manager
<br/><br/><br/>The Institute of Cancer Research | Chester Beatty Laboratories | 237 Fulham Road | London | SW3 6JB
<br/><br/>T +44 7353 5033 | E <a href="/user/SendEmail.jtp?type=node&node=7592336&i=0" target="_top" rel="nofollow" link="external">[hidden email]</a><mailto:<a href="/user/SendEmail.jtp?type=node&node=7592336&i=1" target="_top" rel="nofollow" link="external">[hidden email]</a>> | W www.icr.ac.uk<<a href="http://www.icr.ac.uk/" target="_top" rel="nofollow" link="external">http://www.icr.ac.uk/</a>> | Twitter @ICRnews<<a href="https://twitter.com/ICRnews" target="_top" rel="nofollow" link="external">https://twitter.com/ICRnews</a>>
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<br/>Making the discoveries that defeat cancer
<br/><br/><br/><br/><br/><br/><br/>The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP.
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tag:confocal-microscopy-list.275.s1.nabble.com,2006:post-7592335Free FV300 Scanbox + computer + lasers2021-06-03T15:47:02Z2021-06-03T15:47:02ZMarla Feller
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<br/><br/>We are no longer using a FV300 — used as both 1P and 2P and it was in full working order last time we ran it. We still have 3 original lasers for the system (Argon, HeNe, 405?nm, >10 years of non-use). It’s yours for free if you can PU or can arrange shipping.
<br/><br/>Contact if interested.
<br/><br/><br/>Marla B. Feller, Ph. D.
<br/>Paul Licht Distinguished Professor in Biological Sciences
<br/>Division of Neurobiology, Department of Molecular and Cell Biology & Helen Wills Neuroscience Institute
<br/>University of California, Berkeley
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