Constant Velocity Moves with ASI MS2000 XYZ Stage

[Farid Jalali]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello All,
Not a microscopy question but this group may be able to help. I'd like to program my ASI MS2000 stage to move with a constant velocity and in a specified pattern (eg. ox-plough) in X-Y. We are controlling the stage and our Olympus IX81 DSU with In Vivo from Media Cybernetics at the moment. Stage control is easy enough for imaging, however I am launching a 355nm DPSS laser into the sideport to generate defined tracks of DNA damage. Currently I am launching the laser and manually moving the stage with the joystick. I'd like the velocity and therefore dwell time of the laser to be constant as the beam traverses the cells.

Any thoughts or suggestions would be greatly appreciated.

Cheers
Farid

--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
Princess Margaret Hospital
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
416-581-7754 STTARR at MaRS Building
416-581-7791 STTARR Microscopy Suite

[Michael Cammer]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You will have to overshoot at the edges as acceleration and deceleration
are basic facts.  You can even see this at the edges of some LSCM fields
where the galvos slow down.

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello All,
> Not a microscopy question but this group may be able to help. I'd like to
> program my ASI MS2000 stage to move with a constant velocity and in a
> specified pattern (eg. ox-plough) in X-Y. We are controlling the stage and
> our Olympus IX81 DSU with In Vivo from Media Cybernetics at the moment.
> Stage control is easy enough for imaging, however I am launching a 355nm
> DPSS laser into the sideport to generate defined tracks of DNA damage.
> Currently I am launching the laser and manually moving the stage with the
> joystick. I'd like the velocity and therefore dwell time of the laser to
> be
> constant as the beam traverses the cells.
>
> Any thoughts or suggestions would be greatly appreciated.
>
> Cheers
> Farid
>
> --
> Farid Jalali MSc
> Senior Research Technician/ Lab Manager
> Dr. Robert Bristow Lab
> Applied Molecular Oncology
> Princess Margaret Hospital
> Toronto, Canada
> 416-946-4501 X4351 (Princess Margaret Hospital)
> 416-581-7754 STTARR at MaRS Building
> 416-581-7791 STTARR Microscopy Suite
>

_________________________________________
Michael Cammer   http://www.aecom.yu.edu/aif/

[Hugo.Ostermann]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello Farid,

you might use Image-Pro Plus with the Stage-pro module. I has Basic language
control also for the stage which allows you to program any motion.

 
Mit freundlichen Grüßen,
 
Hugo Ostermann
 
CHROMAPHOR Analysen-Technik GmbH
Mühlenkamp 37
D-59387 Ascheberg - Germany
Tel:   ++49 - 2593-928.600
Fax:  ++49 - 2593-928.601
mail:  [hidden email]
URL: http://www.chromaphor.de

-----Ursprüngliche Nachricht-----
Von: Confocal Microscopy List [mailto:[hidden email]] Im
Auftrag von Michael Cammer
Gesendet: Donnerstag, 31. Januar 2008 06:12
An: [hidden email]
Betreff: Re: Constant Velocity Moves with ASI MS2000 XYZ Stage

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You will have to overshoot at the edges as acceleration and deceleration
are basic facts.  You can even see this at the edges of some LSCM fields
where the galvos slow down.

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello All,
> Not a microscopy question but this group may be able to help. I'd like to
> program my ASI MS2000 stage to move with a constant velocity and in a
> specified pattern (eg. ox-plough) in X-Y. We are controlling the stage and
> our Olympus IX81 DSU with In Vivo from Media Cybernetics at the moment.
> Stage control is easy enough for imaging, however I am launching a 355nm
> DPSS laser into the sideport to generate defined tracks of DNA damage.
> Currently I am launching the laser and manually moving the stage with the
> joystick. I'd like the velocity and therefore dwell time of the laser to
> be
> constant as the beam traverses the cells.
>
> Any thoughts or suggestions would be greatly appreciated.
>
> Cheers
> Farid
>
> --
> Farid Jalali MSc
> Senior Research Technician/ Lab Manager
> Dr. Robert Bristow Lab
> Applied Molecular Oncology
> Princess Margaret Hospital
> Toronto, Canada
> 416-946-4501 X4351 (Princess Margaret Hospital)
> 416-581-7754 STTARR at MaRS Building
> 416-581-7791 STTARR Microscopy Suite
>

_________________________________________
Michael Cammer   http://www.aecom.yu.edu/aif/