CoolLED

[H. de Bont]

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Hello All,

 

We are buying a new confocal/epi microscope.

 

And are interested in experience of users with the CoolLED system.

Do you recommend the CoolLED system over a metal halide lamp?

We want to use the system with epi fluorescence of live cells with Hoechst,
GFP and Cy3 channels.

 

 

Thanks,

 

Hans de Bont

Dept of Toxicology

LACDR

University of Leiden

Einsteinweg 55

2333 CC  Leiden

The Netherlands

Tel: [phone]

Mobile: [phone]

 

[Csúcs Gábor]

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Hello Hans,

We have/had very good experiences with the CoolLed, so I'd not hesitate
too much to go with it. Its only weakness is that it offers less intensity
then a typical (strong) metal-halide lamp. This may be a problem if you
use low NA low mag.oObjectives. For the 63x or 40x oil-immersion typically
used on a confocal this is not an issue.

Cheers   Gabor

Light Microscopy Centre
ETH Zurich,
Schafmattstrasse 18
CH-8093, Zurich
Switzerland

Phone: [phone]







On 6/29/11 9:56 AM, "H. de Bont" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hello All,
>
>
>
>We are buying a new confocal/epi microscope.
>
>
>
>And are interested in experience of users with the CoolLED system.
>
>Do you recommend the CoolLED system over a metal halide lamp?
>
>We want to use the system with epi fluorescence of live cells with
>Hoechst,
>GFP and Cy3 channels.
>
>
>
>
>
>Thanks,
>
>
>
>Hans de Bont
>
>Dept of Toxicology
>
>LACDR
>
>University of Leiden
>
>Einsteinweg 55
>
>2333 CC  Leiden
>
>The Netherlands
>
>Tel: [phone]
>
>Mobile: [phone]
>
>

[lgelman]

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Hans,

Basically, same answer as Gabor... We are happy with ours. We have
CoolLEDs both on our confocals (for visual inspection, this is more than
sufficient) and on some of our wide-field live cell imaging setups.
Intensity is lower than a strong metal halide light, but with live cells
you want anyhow to avoid photobleaching and photodamage...

Best regards,

Laurent.


___________________________
Laurent Gelman, PhD
Friedrich Miescher Institut
Facility for Advanced Imaging and Microscopy
WRO 1066.2.16
Maulbeerstrasse 66
CH-4058 Basel
[phone]



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of H. de Bont
Sent: mercredi 29 juin 2011 09:56
To: [hidden email]
Subject: CoolLED

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello All,

 

We are buying a new confocal/epi microscope.

 

And are interested in experience of users with the CoolLED system.

Do you recommend the CoolLED system over a metal halide lamp?

We want to use the system with epi fluorescence of live cells with
Hoechst,
GFP and Cy3 channels.

 

 

Thanks,

 

Hans de Bont

Dept of Toxicology

LACDR

University of Leiden

Einsteinweg 55

2333 CC  Leiden

The Netherlands

Tel: [phone]

Mobile: [phone]

 

[mcammer]

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Have you tried IRM with the CoolLED system?
Thanks!
-Michael
_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gelman, Laurent
Sent: Friday, July 01, 2011 8:54 AM
To: [hidden email]
Subject: Re: CoolLED

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Hans,

Basically, same answer as Gabor... We are happy with ours. We have
CoolLEDs both on our confocals (for visual inspection, this is more than
sufficient) and on some of our wide-field live cell imaging setups.
Intensity is lower than a strong metal halide light, but with live cells
you want anyhow to avoid photobleaching and photodamage...

Best regards,

Laurent.


___________________________
Laurent Gelman, PhD
Friedrich Miescher Institut
Facility for Advanced Imaging and Microscopy
WRO 1066.2.16
Maulbeerstrasse 66
CH-4058 Basel
[phone]



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of H. de Bont
Sent: mercredi 29 juin 2011 09:56
To: [hidden email]
Subject: CoolLED

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello All,

 

We are buying a new confocal/epi microscope.

 

And are interested in experience of users with the CoolLED system.

Do you recommend the CoolLED system over a metal halide lamp?

We want to use the system with epi fluorescence of live cells with
Hoechst,
GFP and Cy3 channels.

 

 

Thanks,

 

Hans de Bont

Dept of Toxicology

LACDR

University of Leiden

Einsteinweg 55

2333 CC  Leiden

The Netherlands

Tel: [phone]

Mobile: [phone]

 

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