Coverslip rack/holder for nitric acid washes

[Daniel Murphy]

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Hello,

I am wondering if anyone has suggestions/thoughts/experience regarding a
good coverslip holder/rack that is made out a material that will not be
etched by nitric acid.  In our lab we currently wash our coverslips very
carefully, following a protocol that involves a nitric acid wash step, and a
lot more.  In order to make the entire process a bit more efficient and less
tedious, I am looking into coverslip holders/racks that the coverslips could
just sit in throughout the whole procedure (primarily for water washes,
nitric acid washes, and a drying step.  These racks would need to be:
- low-contact so that the entire coverslip surface is well exposed for
washes, etches, and drying steps
- made of a material that is not corroded by nitric acid (but also not gold
or platinum!)
- high-capacity (able to hold 50-100 coverslips at least)

Does anyone have experience using one that works really well?  I'm guessing
that there are adequate staining racks available for this, but it would be
best to know which one has worked for other people.

Any thoughts suggestions would be very much appreciated!  

Thanks!

Dan

[Elena Rebollo Arredondo]

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Hi,

 Porcelain Staining Racks
ANAME
ref. 8542-E40

 Could you please provide your protocol for cleaning coverslips with the
nitric acid and the nitric reference?

thanks,

Elena

On 15 December 2011 16:25, Daniel Murphy <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
>
> I am wondering if anyone has suggestions/thoughts/experience regarding a
> good coverslip holder/rack that is made out a material that will not be
> etched by nitric acid.  In our lab we currently wash our coverslips very
> carefully, following a protocol that involves a nitric acid wash step, and
> a
> lot more.  In order to make the entire process a bit more efficient and
> less
> tedious, I am looking into coverslip holders/racks that the coverslips
> could
> just sit in throughout the whole procedure (primarily for water washes,
> nitric acid washes, and a drying step.  These racks would need to be:
> - low-contact so that the entire coverslip surface is well exposed for
> washes, etches, and drying steps
> - made of a material that is not corroded by nitric acid (but also not gold
> or platinum!)
> - high-capacity (able to hold 50-100 coverslips at least)
>
> Does anyone have experience using one that works really well?  I'm guessing
> that there are adequate staining racks available for this, but it would be
> best to know which one has worked for other people.
>
> Any thoughts suggestions would be very much appreciated!
>
> Thanks!
>
> Dan
>

--
Elena Rebollo Ph.D.
Confocal Microscopy Unit IBMB-PCB
Molecular Biology Institute of Barcelona (IBMB), CSIC
c/ Baldiri Reixac,10-12
08028 Barcelona (Spain)
Tel [phone]
www.ibmb.csic.es<http://www.ibmb.csic.es/index.php?pg=laboratorio&idLaboratorio=27&tab=lab_home>

[Chen, De (NIH/NCI) [C]]

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You can try the following:

Corrosion-resistant staining rack for holding coverslips (Thomas Scientific,
cat. no. 8542E40)


________________________________________
From: Daniel Murphy [[hidden email]]
Sent: Thursday, December 15, 2011 10:25 AM
To: [hidden email]
Subject: Coverslip rack/holder for nitric acid washes

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Hello,

I am wondering if anyone has suggestions/thoughts/experience regarding a
good coverslip holder/rack that is made out a material that will not be
etched by nitric acid.  In our lab we currently wash our coverslips very
carefully, following a protocol that involves a nitric acid wash step, and a
lot more.  In order to make the entire process a bit more efficient and less
tedious, I am looking into coverslip holders/racks that the coverslips could
just sit in throughout the whole procedure (primarily for water washes,
nitric acid washes, and a drying step.  These racks would need to be:
- low-contact so that the entire coverslip surface is well exposed for
washes, etches, and drying steps
- made of a material that is not corroded by nitric acid (but also not gold
or platinum!)
- high-capacity (able to hold 50-100 coverslips at least)

Does anyone have experience using one that works really well?  I'm guessing
that there are adequate staining racks available for this, but it would be
best to know which one has worked for other people.

Any thoughts suggestions would be very much appreciated!

Thanks!

Dan

[Gabriel Lapointe-3]

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We use these porcelain rack, 12 place per rack and expansive but really
worth it.
http://www1.thomassci.com:8080/catalog/product/4076
No commercial interest, just an happy customer.

   *Gabriel Lapointe, M.Sc.*
Lab Manager
Concordia University, Biology Department
7141 Sherbrooke St. West SP 534
Montréal QC H4B 1R6 Canada
Lab : [phone] x5988
Fax : [phone]
Cell : [phone]
[hidden email]
http://gabriellapointe.ca



2011/12/15 Elena Rebollo Arredondo <[hidden email]>

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
>
>  Porcelain Staining Racks
> ANAME
> ref. 8542-E40
>
>  Could you please provide your protocol for cleaning coverslips with the
> nitric acid and the nitric reference?
>
> thanks,
>
> Elena
>
> On 15 December 2011 16:25, Daniel Murphy <[hidden email]
> >wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hello,
> >
> > I am wondering if anyone has suggestions/thoughts/experience regarding a
> > good coverslip holder/rack that is made out a material that will not be
> > etched by nitric acid.  In our lab we currently wash our coverslips very
> > carefully, following a protocol that involves a nitric acid wash step,
> and
> > a
> > lot more.  In order to make the entire process a bit more efficient and
> > less
> > tedious, I am looking into coverslip holders/racks that the coverslips
> > could
> > just sit in throughout the whole procedure (primarily for water washes,
> > nitric acid washes, and a drying step.  These racks would need to be:
> > - low-contact so that the entire coverslip surface is well exposed for
> > washes, etches, and drying steps
> > - made of a material that is not corroded by nitric acid (but also not
> gold
> > or platinum!)
> > - high-capacity (able to hold 50-100 coverslips at least)
> >
> > Does anyone have experience using one that works really well?  I'm
> guessing
> > that there are adequate staining racks available for this, but it would
> be
> > best to know which one has worked for other people.
> >
> > Any thoughts suggestions would be very much appreciated!
> >
> > Thanks!
> >
> > Dan
> >
>
>
>
> --
> Elena Rebollo Ph.D.
> Confocal Microscopy Unit IBMB-PCB
> Molecular Biology Institute of Barcelona (IBMB), CSIC
> c/ Baldiri Reixac,10-12
> 08028 Barcelona (Spain)
> Tel [phone]
> www.ibmb.csic.es<
> http://www.ibmb.csic.es/index.php?pg=laboratorio&idLaboratorio=27&tab=lab_home
> >
>

[jlribas]

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Dear Dan,
You can find a lot of suggestions at:
http://www.emsdiasum.com/microscopy/products/histology/staining.aspx
No commercial interest.

Best
Juan Luis


El 15/12/2011 16:25, Daniel Murphy escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
>
> I am wondering if anyone has suggestions/thoughts/experience regarding a
> good coverslip holder/rack that is made out a material that will not be
> etched by nitric acid.  In our lab we currently wash our coverslips very
> carefully, following a protocol that involves a nitric acid wash step, and a
> lot more.  In order to make the entire process a bit more efficient and less
> tedious, I am looking into coverslip holders/racks that the coverslips could
> just sit in throughout the whole procedure (primarily for water washes,
> nitric acid washes, and a drying step.  These racks would need to be:
> - low-contact so that the entire coverslip surface is well exposed for
> washes, etches, and drying steps
> - made of a material that is not corroded by nitric acid (but also not gold
> or platinum!)
> - high-capacity (able to hold 50-100 coverslips at least)
>
> Does anyone have experience using one that works really well?  I'm guessing
> that there are adequate staining racks available for this, but it would be
> best to know which one has worked for other people.
>
> Any thoughts suggestions would be very much appreciated!
>
> Thanks!
>
> Dan
>

--
Juan Luis Ribas
Servicio de Microscopía
Centro de Investigación, Tecnología e Innovación
Universidad de Sevilla
Av. Reina Mercedes 4b
41012 Sevilla

Tfno: 954559983

[Cameron, Lisa]

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Hello -

I have a laser stability and low power error with my Coherent Chameleon Ultra
Vision II laser and I can not change wavelengths without getting a low power
error.
Coherent service has been here a couple times and I am happy with the service
calls.
The "fix" has been determined as replace the laser.
However the ship date for my new laser is Feb 10, 2012!

I was wondering what other people's experience has been with delivery time on
replacement Chameleon Ultra Vision lasers.

This is a long time for my facility 2-photon laser to be down.

Thanks,
Lisa
 


---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute
Boston, MA 02215
[hidden email]
Microscopy.dfci.harvard.edu


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.

[lechristophe]

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Hi,

We use porcelain / ceramic white racks for coverslips from Thomas Scientific. They are a bit expensive and hold only 12 / 24 coverslips though.

Le 15 déc. 2011 à 16:25, Daniel Murphy <[hidden email]> a écrit :

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
>
> I am wondering if anyone has suggestions/thoughts/experience regarding a
> good coverslip holder/rack that is made out a material that will not be
> etched by nitric acid.  In our lab we currently wash our coverslips very
> carefully, following a protocol that involves a nitric acid wash step, and a
> lot more.  In order to make the entire process a bit more efficient and less
> tedious, I am looking into coverslip holders/racks that the coverslips could
> just sit in throughout the whole procedure (primarily for water washes,
> nitric acid washes, and a drying step.  These racks would need to be:
> - low-contact so that the entire coverslip surface is well exposed for
> washes, etches, and drying steps
> - made of a material that is not corroded by nitric acid (but also not gold
> or platinum!)
> - high-capacity (able to hold 50-100 coverslips at least)
>
> Does anyone have experience using one that works really well?  I'm guessing
> that there are adequate staining racks available for this, but it would be
> best to know which one has worked for other people.
>
> Any thoughts suggestions would be very much appreciated!  
>
> Thanks!
>
> Dan

[Tobias Baskin]

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Yes,
        We use those porcelain racks too and they
are excellent. But the capacity is low and the
cost high. Be worth checking whether a local
potter (who makes porcelain rather than
earthenware) could knock you up one or two larger
ones. I suspect they could and it would cost far
less than the commercial ones.
        As ever
                Tobias

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hi,
>
>We use porcelain / ceramic white racks for
>coverslips from Thomas Scientific. They are a
>bit expensive and hold only 12 / 24 coverslips
>though.
>
>Le 15 déc. 2011 à 16:25, Daniel Murphy
><[hidden email]> a écrit :
>
>>  *****
>>  To join, leave or search the confocal microscopy listserv, go to:
>>  http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>  *****
>>
>>  Hello,
>>
>>  I am wondering if anyone has suggestions/thoughts/experience regarding a
>>  good coverslip holder/rack that is made out a material that will not be
>>  etched by nitric acid.  In our lab we currently wash our coverslips very
>>  carefully, following a protocol that involves a nitric acid wash step, and a
>>  lot more.  In order to make the entire process a bit more efficient and less
>>  tedious, I am looking into coverslip holders/racks that the coverslips could
>>  just sit in throughout the whole procedure (primarily for water washes,
>>  nitric acid washes, and a drying step.  These racks would need to be:
>>  - low-contact so that the entire coverslip surface is well exposed for
>>  washes, etches, and drying steps
>>  - made of a material that is not corroded by nitric acid (but also not gold
>>  or platinum!)
>>  - high-capacity (able to hold 50-100 coverslips at least)
>>
>>  Does anyone have experience using one that works really well?  I'm guessing
>>  that there are adequate staining racks available for this, but it would be
>>  best to know which one has worked for other people.
>>
>>  Any thoughts suggestions would be very much appreciated!
>>
>>  Thanks!
>>
>>  Dan

--
       _      ____          __   ____
      /  \   /          / \    /   \ \       Tobias I. Baskin
     /   /  /          /   \   \      \        Professor
    /_ /   __      /__ \   \       \__    Biology Department
   /      /          /       \   \       \        611 N. Pleasant St.
  /      /          /         \   \       \
University of Massachusetts
/      / ___   /           \   \__/  \ ____ Amherst, MA, 01003
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

[Pascal Weber]

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I never worked on Coherent pulsed laser. But it's always the same sound, it
takes a long time to repare it. From my experience Spectra gave me a laser
while they repaired the old one. Congratulations Spectra ! Most of the time
they repared it on site.

[Molnár Dávid]

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Dear Confocal List Collegues!
 
I'd greatly appreciate if anybody could help me:
I'm looking for any existing database files, or
dataset of discrete values / functions that could
be used for describing the absorption and emission
spectra of fluorescent dyes to use for further
calculations and optimization.
(Zen and other softwares have built-in modules for dye
characteristics - as I remember. I don't know how its
database was edited)

I'm not advanced at programming but
 I'd like to write a short script, mainly
for educational and optimization purposes.
That's why I'm seeking for an available database, or
any advices.
 
 
Thank You in advance!
David
 
 
 
 
Dávid Molnár M.D.
Scientific Assistant Fellow
Department of Human Morphology and Developmental Biology
Semmelweis University, Faculty of Medicine
58. Tűzoltó st
Budapest
H-1094
Hungary
 
e-mail: molnar.david[at]med.semmelweis-univ.hu
web: anatomia.sote.hu/devbiollab/

[Emmanuel Gustin]

David,

You may be attempting to reinvent the wheel? There are already some many on-line resources for spectra... Commercial but freely accessible ones are provided by Invitrogen, BD, Zeiss, and eBioscience; among others. These don't allow you to download the database files, however.

If you are looking for downloadable spectra files, I would suggest PubSpectra:
http://home.earthlink.net/~pubspectra/
and the spectrum viewer of the University of Arizona:
http://www.spx.arizona.edu/spectra/

Best Regards,

Emmanuel

--
 Emmanuel Gustin,    Tel. [phone],    e-mail: [hidden email]  


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Molnár Dávid
Sent: vrijdag 16 december 2011 09:43
To: [hidden email]
Subject: dye description database

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Dear Confocal List Collegues!
 
I'd greatly appreciate if anybody could help me:
I'm looking for any existing database files, or
dataset of discrete values / functions that could
be used for describing the absorption and emission
spectra of fluorescent dyes to use for further
calculations and optimization.
(Zen and other softwares have built-in modules for dye
characteristics - as I remember. I don't know how its
database was edited)

I'm not advanced at programming but
 I'd like to write a short script, mainly
for educational and optimization purposes.
That's why I'm seeking for an available database, or
any advices.
 
 
Thank You in advance!
David
 
 
 
 
Dávid Molnár M.D.
Scientific Assistant Fellow
Department of Human Morphology and Developmental Biology
Semmelweis University, Faculty of Medicine
58. Tűzoltó st
Budapest
H-1094
Hungary
 
e-mail: molnar.david[at]med.semmelweis-univ.hu
web: anatomia.sote.hu/devbiollab/

[George McNamara]

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Hi David,

The zip file at the top of

http://sylvester.org/shared-resources/analytical-imaging/links-forms-documentation/pubspectra-data

is the source of the Zeiss ZEN fluorophores dataset.

I have some new spectra as data on my computers, but most researchers
only publish graphs, not numerical data files.

Urs Utzinger and Carl Boswell have added a few spectra - most notably
multiphoton excitation spectra - downloadable from
http://www.spectra.arizona.edu/


Enjoy,

George



On 12/16/2011 3:42 AM, Molnár Dávid wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Confocal List Collegues!
>
> I'd greatly appreciate if anybody could help me:
> I'm looking for any existing database files, or
> dataset of discrete values / functions that could
> be used for describing the absorption and emission
> spectra of fluorescent dyes to use for further
> calculations and optimization.
> (Zen and other softwares have built-in modules for dye
> characteristics - as I remember. I don't know how its
> database was edited)
>
> I'm not advanced at programming but
>   I'd like to write a short script, mainly
> for educational and optimization purposes.
> That's why I'm seeking for an available database, or
> any advices.
>
>
> Thank You in advance!
> David
>
>
>
>
> Dávid Molnár M.D.
> Scientific Assistant Fellow
> Department of Human Morphology and Developmental Biology
> Semmelweis University, Faculty of Medicine
> 58. Tűzoltó st
> Budapest
> H-1094
> Hungary
>
> e-mail: molnar.david[at]med.semmelweis-univ.hu
> web: anatomia.sote.hu/devbiollab/
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

[George McNamara]

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My experience with Coherent has been great. When the service engineer
decided it was time for our Coherent Chameleon Ultra II (2.2 Watts ~840
nm) to be replaced under service contract, he had the factory in
Scotland ship in a complete replacement unit (a refurbished unit), we
wrestled the old one out, the new one in, Leica service came down a
couple of days later to align it, and we are happily pulsing at 4.8
Watts at ~840 nm. The longest delay was getting it out of U.S. customs.
Coherent refurbishes its MP lasers to "new factory specifications"
before shipping them out as replacement units.

On 12/16/2011 2:19 AM, Pascal Weber wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I never worked on Coherent pulsed laser. But it's always the same sound, it
> takes a long time to repare it. From my experience Spectra gave me a laser
> while they repaired the old one. Congratulations Spectra ! Most of the time
> they repared it on site.
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

[Theresa Swayne]

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Hi Lisa,

You're not alone in this. We're waiting on a replacement of the same laser. They placed the request Sept. 20. Ship date was originally 25 November, but then it was changed to 16 December.  Coherent said they're sending a new (as opposed to refurbished) laser, but that's not a great consolation to me. A field-swappable laser seems like a good idea, but it pays off for users only when the factory can maintain sufficient inventory of replacements.

Cheers,
Theresa

On Dec 15, 2011, at 12:35 PM, Cameron, Lisa wrote:

> However the ship date for my new laser is Feb 10, 2012!
>
> I was wondering what other people's experience has been with delivery time on
> replacement Chameleon Ultra Vision lasers.


------------------------------------
Theresa C. Swayne, Ph.D.
Manager, Confocal and Specialized Microscopy Shared Resource
Herbert Irving Comprehensive Cancer Center, Columbia University
1130 Saint Nicholas Ave, 222A
New York, NY 10032

212-851-4613

[hidden email]
http://hiccc.columbia.edu/research/sharedresources/confocal

[Cameron, Lisa]

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Just thought I'd let you all know the update from Coherent -

Both my service rep and sales rep called me today to let me know that they are
shipping me a temporary loaner laser and will install it on Tues (Dec 20). Also
ship date on my new laser has been moved up to mid-early January.

Definitely impressed with Coherent service and help getting me back up and
running.

- Lisa


---------------------------------------
Lisa Cameron, Ph.D.
Dana Farber Cancer Institute
Boston, MA 02215

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Cameron, Lisa
Sent: Thursday, December 15, 2011 12:36 PM
To: [hidden email]
Subject: [CONFOCALMICROSCOPY] 2-p laser delivery question

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Hello -

I have a laser stability and low power error with my Coherent Chameleon Ultra
Vision II laser and I can not change wavelengths without getting a low power
error.
Coherent service has been here a couple times and I am happy with the service
calls.
The "fix" has been determined as replace the laser.
However the ship date for my new laser is Feb 10, 2012!

I was wondering what other people's experience has been with delivery time on
replacement Chameleon Ultra Vision lasers.

This is a long time for my facility 2-photon laser to be down.

Thanks,
Lisa
 


---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute
Boston, MA 02215
[hidden email]
Microscopy.dfci.harvard.edu


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.

[Zac Arrac Atelaz]

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****


That sounds like good service!!! thank you for sharing!
 > Date: Fri, 16 Dec 2011 18:56:03 -0500

> From: [hidden email]
> Subject: Re: 2-p laser delivery question - update
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Just thought I'd let you all know the update from Coherent -
>
> Both my service rep and sales rep called me today to let me know that they are
> shipping me a temporary loaner laser and will install it on Tues (Dec 20). Also
> ship date on my new laser has been moved up to mid-early January.
>
> Definitely impressed with Coherent service and help getting me back up and
> running.
>
> - Lisa
>
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Dana Farber Cancer Institute
> Boston, MA 02215
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Cameron, Lisa
> Sent: Thursday, December 15, 2011 12:36 PM
> To: [hidden email]
> Subject: [CONFOCALMICROSCOPY] 2-p laser delivery question
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello -
>
> I have a laser stability and low power error with my Coherent Chameleon Ultra
> Vision II laser and I can not change wavelengths without getting a low power
> error.
> Coherent service has been here a couple times and I am happy with the service
> calls.
> The "fix" has been determined as replace the laser.
> However the ship date for my new laser is Feb 10, 2012!
>
> I was wondering what other people's experience has been with delivery time on
> replacement Chameleon Ultra Vision lasers.
>
> This is a long time for my facility 2-photon laser to be down.
>
> Thanks,
> Lisa
>  
>
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
> Boston, MA 02215
> [hidden email]
> Microscopy.dfci.harvard.edu
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.

     

[Jeremy Adler-4]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

invitrogen (Molecular Probes) sell a teflon rack for coverslips, but  
it only holds 8.


  C-14784
List Price
     (USD) 78.25
     My Price & Availability


Jeremy Adler
IGP
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden

0046 (0)18 471 4607

[Clements, Ian]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Actually they also have

C24784 coverslip maxi-rack *for 50 coverslips*
http://products.invitrogen.com/ivgn/product/C24784


Ian Clements

-----Original Message-----
From: Jeremy Adler [mailto:[hidden email]]
Sent: Monday, December 19, 2011 2:27 AM
To: [hidden email]
Subject: Re: Coverslip rack/holder for nitric acid washes

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

invitrogen (Molecular Probes) sell a teflon rack for coverslips, but
it only holds 8.


  C-14784
List Price
     (USD) 78.25
     My Price & Availability


Jeremy Adler
IGP
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden

0046 (0)18 471 4607

This email message, together with any attachments, is for the sole use of the intended recipient(s) and is the confidential information of Applied Precision Inc. If you are not the intended recipient, your review, use, disclosure, copying or dissemination of this email message or its attachments, or the information contained therein, is strictly prohibited. If you are not the intended recipient or if you think this email was sent to you in error, please notify the sender by reply email and delete this message and its attachments, as well as all copies, from your system.

[Kilgore, Jason-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

** Vendor Response **

Actually, the Maxi-Rack hasn't been validated for nitric acid washes, so we don't know how well the glue and plastic would hold up.  May be fine, we just haven't tested it.  We do know that it doesn't do well with organic solvents.

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes Labeling and Detection Technologies
Cells Systems Division
 
T 1 800 955 6288 then option 4, then option 6,  or  541 335 0353 . F 541 335 0238
29851 Willow Creek Rd . Eugene . OR . 97402-9132 . United States
www.invitrogen.com/technicalsupport

 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Clements, Ian
Sent: Monday, December 19, 2011 11:03 AM
To: [hidden email]
Subject: Re: Coverslip rack/holder for nitric acid washes

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Actually they also have

C24784 coverslip maxi-rack *for 50 coverslips*
http://products.invitrogen.com/ivgn/product/C24784


Ian Clements

-----Original Message-----
From: Jeremy Adler [mailto:[hidden email]]
Sent: Monday, December 19, 2011 2:27 AM
To: [hidden email]
Subject: Re: Coverslip rack/holder for nitric acid washes

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

invitrogen (Molecular Probes) sell a teflon rack for coverslips, but
it only holds 8.


  C-14784
List Price
     (USD) 78.25
     My Price & Availability


Jeremy Adler
IGP
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden

0046 (0)18 471 4607

This email message, together with any attachments, is for the sole use of the intended recipient(s) and is the confidential information of Applied Precision Inc. If you are not the intended recipient, your review, use, disclosure, copying or dissemination of this email message or its attachments, or the information contained therein, is strictly prohibited. If you are not the intended recipient or if you think this email was sent to you in error, please notify the sender by reply email and delete this message and its attachments, as well as all copies, from your system.

[Jeremy Adler-4]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Invitrogen's 50 coverslip rack is plastic rather than teflon, and  
would succumb to organic solvents.


Quoting "Clements, Ian" <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Actually they also have
>
> C24784 coverslip maxi-rack *for 50 coverslips*
> http://products.invitrogen.com/ivgn/product/C24784
>
>
> Ian Clements
>
> -----Original Message-----
> From: Jeremy Adler [mailto:[hidden email]]
> Sent: Monday, December 19, 2011 2:27 AM
> To: [hidden email]
> Subject: Re: Coverslip rack/holder for nitric acid washes
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> invitrogen (Molecular Probes) sell a teflon rack for coverslips, but
> it only holds 8.
>
>
>   C-14784
> List Price
>      (USD) 78.25
>      My Price & Availability
>
>
> Jeremy Adler
> IGP
> Rudbeckslaboratoriet
> Daghammersköljdsväg 20
> 751 85 Uppsala
> Sweden
>
> 0046 (0)18 471 4607
>
> This email message, together with any attachments, is for the sole  
> use of the intended recipient(s) and is the confidential information  
> of Applied Precision Inc. If you are not the intended recipient,  
> your review, use, disclosure, copying or dissemination of this email  
> message or its attachments, or the information contained therein, is  
> strictly prohibited. If you are not the intended recipient or if you  
> think this email was sent to you in error, please notify the sender  
> by reply email and delete this message and its attachments, as well  
> as all copies, from your system.
>

Jeremy Adler
IGP
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden

0046 (0)18 471 4607