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Coverslips for Primary Neuronal Cultures

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Claire Brown

Coverslips for Primary Neuronal Cultures

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A colleague of mine asked me this but I'm not familiar with "D" Coverslips.

I thought someone here might know.

For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
If you have any recommendations and insights, or other suppliers to consider, I'm all ears.

Sincerely,

Claire

Dan Focht

Re: Coverslips for Primary Neuronal Cultures

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Claire

You are correct D263 is the currently used name for Desag 263 glass It is a Schott glass from Germany. It has been the standard for cell compatibility for over 32 years that I know of. It is the only type of coverglass we use in our Bioptechs products. What size do you need?

Dan

On Jan 20, 2020, at 3:59 PM, Claire Brown, Dr. <[hidden email]> wrote:

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A colleague of mine asked me this but I'm not familiar with "D" Coverslips.

I thought someone here might know.

For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
If you have any recommendations and insights, or other suppliers to consider, I'm all ears.

Sincerely,

Claire

Dan Focht
Bioptechs Inc.
3560 Beck Road
Butler, PA 16002-9259
Office: 724-282-7145
Toll Free: 877-LIVE-CELL (548-3235)
[hidden email]
www.bioptechs.com

Julia Edgar

Re: Coverslips for Primary Neuronal Cultures

In reply to this post by Claire Brown

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Hi Claire
I can't answer you actual question, but this information might still be helpful.
In my experience, neurons adhere much better to glass coverslips coated with poly-L-lysine in boric acid buffer versus poly-L-lysine in water. The difference is remarkable.
Best wishes
Julia

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Claire Brown, Dr.
Sent: 20 January 2020 20:59
To: [hidden email]
Subject: Coverslips for Primary Neuronal Cultures

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

A colleague of mine asked me this but I'm not familiar with "D" Coverslips.

I thought someone here might know.

For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
If you have any recommendations and insights, or other suppliers to consider, I'm all ears.

Sincerely,

Claire

sparker

Re: Coverslips for Primary Neuronal Cultures

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Hi Everyone,

I use coverglass from Carolina Biological Supply for my primary neuron cultures, coated in poly-L-lysine. According to their site, it’s the "the famous Deutsche Spiegelglas.” Maybe the D stands for Germany :) ! Good luck! https://www.carolina.com/catalog/detail.jsp?prodId=633037&s_cid=ppc_gl_products&utm_source=google&utm_medium=cpc&scid=scplp633037&sc_intid=633037&gclid=CjwKCAjwnrjrBRAMEiwAXsCc48U2hkpOztMo9SuwpAQWeg1rqXsF8Hye8tA_TgnKua9gIfHuswOSBhoCFEIQAvD_BwE

Sara Parker
Postdoctoral Fellow, Mouneimne Lab
(520) 626-3860
[hidden email]
Department of Cellular & Molecular Medicine
University of Arizona
On Jan 20, 2020, 2:09 PM -0700, Julia Edgar <[hidden email]>, wrote:
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Claire
I can't answer you actual question, but this information might still be helpful.
In my experience, neurons adhere much better to glass coverslips coated with poly-L-lysine in boric acid buffer versus poly-L-lysine in water. The difference is remarkable.
Best wishes
Julia

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Claire Brown, Dr.
Sent: 20 January 2020 20:59
To: [hidden email]
Subject: Coverslips for Primary Neuronal Cultures

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

A colleague of mine asked me this but I'm not familiar with "D" Coverslips.

I thought someone here might know.

For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
If you have any recommendations and insights, or other suppliers to consider, I'm all ears.

Sincerely,

Claire

David Crossman

Re: Coverslips for Primary Neuronal Cultures

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Post images on http://www.imgur.com and include the link in your posting.
*****

Does the D stand for poly-D-lysine? This is the enantiomer or mirror image of ploy-L-lysine. It has the exact same chemical characteristics for coating and binding cells but is thought to be less likely to be metabolised by the cells in culture as this enantiomer is not found in nature.

Either version can be bought from https://www.neuvitro.com/

Cheers,
David

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Parker, Sara - (saraparker)
Sent: Tuesday, 21 January 2020 12:02 PM
To: [hidden email]
Subject: Re: Coverslips for Primary Neuronal Cultures

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Everyone,

I use coverglass from Carolina Biological Supply for my primary neuron cultures, coated in poly-L-lysine. According to their site, it’s the "the famous Deutsche Spiegelglas.” Maybe the D stands for Germany :) ! Good luck! https://www.carolina.com/catalog/detail.jsp?prodId=633037&s_cid=ppc_gl_products&utm_source=google&utm_medium=cpc&scid=scplp633037&sc_intid=633037&gclid=CjwKCAjwnrjrBRAMEiwAXsCc48U2hkpOztMo9SuwpAQWeg1rqXsF8Hye8tA_TgnKua9gIfHuswOSBhoCFEIQAvD_BwE

Sara Parker
Postdoctoral Fellow, Mouneimne Lab
(520) 626-3860
[hidden email]
Department of Cellular & Molecular Medicine University of Arizona On Jan 20, 2020, 2:09 PM -0700, Julia Edgar <[hidden email]>, wrote:
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Claire
I can't answer you actual question, but this information might still be helpful.
In my experience, neurons adhere much better to glass coverslips coated with poly-L-lysine in boric acid buffer versus poly-L-lysine in water. The difference is remarkable.
Best wishes
Julia

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Claire Brown, Dr.
Sent: 20 January 2020 20:59
To: [hidden email]
Subject: Coverslips for Primary Neuronal Cultures

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

A colleague of mine asked me this but I'm not familiar with "D" Coverslips.

I thought someone here might know.

For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
If you have any recommendations and insights, or other suppliers to consider, I'm all ears.

Sincerely,

Claire

Cammer, Michael

Re: Coverslips for Primary Neuronal Cultures

In reply to this post by sparker

*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

A few months ago, when dealing with a lab that said it was "impossible" to grow neurons on glass, I tallied some searches. Most of the papers cited poly-L-lysine and a few other coatings such as fibronectin.

The labs I have worked with, dating back to the 1990s pre-GFP, have grown various types of neuronal cultures in chambers such as MatTek dishes.

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016

[hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/

Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Parker, Sara - (saraparker) <[hidden email]>
Sent: Monday, January 20, 2020 6:01:53 PM
To: [hidden email]
Subject: Re: Coverslips for Primary Neuronal Cultures

[EXTERNAL]

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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=V-QRS_Oa0GE2D9iHdSkx5V5igyYVFl3EAs_T8-uJB48&e= and include the link in your posting.
*****

Hi Everyone,

I use coverglass from Carolina Biological Supply for my primary neuron cultures, coated in poly-L-lysine. According to their site, it’s the "the famous Deutsche Spiegelglas.” Maybe the D stands for Germany :) ! Good luck! https://urldefense.proofpoint.com/v2/url?u=https-3A__www.carolina.com_catalog_detail.jsp-3FprodId-3D633037-26s-5Fcid-3Dppc-5Fgl-5Fproducts-26utm-5Fsource-3Dgoogle-26utm-5Fmedium-3Dcpc-26scid-3Dscplp633037-26sc-5Fintid-3D633037-26gclid-3DCjwKCAjwnrjrBRAMEiwAXsCc48U2hkpOztMo9SuwpAQWeg1rqXsF8Hye8tA-5FTgnKua9gIfHuswOSBhoCFEIQAvD-5FBwE&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=yVYoHXmgwizOON6rENNgNiuRPsNrmTCzUFz-m8ZXsjE&e=

Sara Parker
Postdoctoral Fellow, Mouneimne Lab
(520) 626-3860
[hidden email]
Department of Cellular & Molecular Medicine
University of Arizona
On Jan 20, 2020, 2:09 PM -0700, Julia Edgar <[hidden email]>, wrote:
*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=qGzLgNJIbD8fhDd7B1PhDwpLska7gSXdoineUkYLOQA&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=V-QRS_Oa0GE2D9iHdSkx5V5igyYVFl3EAs_T8-uJB48&e= and include the link in your posting.
*****

Hi Claire
I can't answer you actual question, but this information might still be helpful.
In my experience, neurons adhere much better to glass coverslips coated with poly-L-lysine in boric acid buffer versus poly-L-lysine in water. The difference is remarkable.
Best wishes
Julia

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Claire Brown, Dr.
Sent: 20 January 2020 20:59
To: [hidden email]
Subject: Coverslips for Primary Neuronal Cultures

*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=qGzLgNJIbD8fhDd7B1PhDwpLska7gSXdoineUkYLOQA&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=V-QRS_Oa0GE2D9iHdSkx5V5igyYVFl3EAs_T8-uJB48&e= and include the link in your posting.
*****

A colleague of mine asked me this but I'm not familiar with "D" Coverslips.

I thought someone here might know.

For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
If you have any recommendations and insights, or other suppliers to consider, I'm all ears.

Sincerely,

Claire

Antonius M J Van Dongen

Re: Coverslips for Primary Neuronal Cultures

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Post images on http://www.imgur.com and include the link in your posting.
*****

We have grown rat E18 neurons for decades on glass coated with poly -D-lysine. Coating is tricky though: it requires pretreatment with a strong acid. We therefore usually order PDL-coated dishes from Mattek, but still re-coat them with fresh PDL on the day of the plating.

For multi-well plates we now use plastic bottom dishes with coverslip thickness plastic which can be imaged with oil objectives. Neurons love growing on plastic.

Tony

> On 21 Jan 2020, at 09:14, Cammer, Michael <[hidden email]> wrote:
>
> - External Email -
>
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> A few months ago, when dealing with a lab that said it was "impossible" to grow neurons on glass, I tallied some searches. Most of the papers cited poly-L-lysine and a few other coatings such as fibronectin.
>
>
> The labs I have worked with, dating back to the 1990s pre-GFP, have grown various types of neuronal cultures in chambers such as MatTek dishes.
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
>
> [hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/
>
> Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
>
>
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of Parker, Sara - (saraparker) <[hidden email]>
> Sent: Monday, January 20, 2020 6:01:53 PM
> To: [hidden email]
> Subject: Re: Coverslips for Primary Neuronal Cultures
>
> [EXTERNAL]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=qGzLgNJIbD8fhDd7B1PhDwpLska7gSXdoineUkYLOQA&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=V-QRS_Oa0GE2D9iHdSkx5V5igyYVFl3EAs_T8-uJB48&e= and include the link in your posting.
> *****
>
> Hi Everyone,
>
> I use coverglass from Carolina Biological Supply for my primary neuron cultures, coated in poly-L-lysine. According to their site, it’s the "the famous Deutsche Spiegelglas.” Maybe the D stands for Germany :) ! Good luck! https://urldefense.proofpoint.com/v2/url?u=https-3A__www.carolina.com_catalog_detail.jsp-3FprodId-3D633037-26s-5Fcid-3Dppc-5Fgl-5Fproducts-26utm-5Fsource-3Dgoogle-26utm-5Fmedium-3Dcpc-26scid-3Dscplp633037-26sc-5Fintid-3D633037-26gclid-3DCjwKCAjwnrjrBRAMEiwAXsCc48U2hkpOztMo9SuwpAQWeg1rqXsF8Hye8tA-5FTgnKua9gIfHuswOSBhoCFEIQAvD-5FBwE&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=yVYoHXmgwizOON6rENNgNiuRPsNrmTCzUFz-m8ZXsjE&e=
>
> Sara Parker
> Postdoctoral Fellow, Mouneimne Lab
> (520) 626-3860
> [hidden email]
> Department of Cellular & Molecular Medicine
> University of Arizona
> On Jan 20, 2020, 2:09 PM -0700, Julia Edgar <[hidden email]>, wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=qGzLgNJIbD8fhDd7B1PhDwpLska7gSXdoineUkYLOQA&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=V-QRS_Oa0GE2D9iHdSkx5V5igyYVFl3EAs_T8-uJB48&e= and include the link in your posting.
> *****
>
> Hi Claire
> I can't answer you actual question, but this information might still be helpful.
> In my experience, neurons adhere much better to glass coverslips coated with poly-L-lysine in boric acid buffer versus poly-L-lysine in water. The difference is remarkable.
> Best wishes
> Julia
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On Behalf Of Claire Brown, Dr.
> Sent: 20 January 2020 20:59
> To: [hidden email]
> Subject: Coverslips for Primary Neuronal Cultures
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=xBoIPE2HCh2ZOfmVqCeuCqdTYEY86DVuWuglrRVSHA8&s=qGzLgNJIbD8fhDd7B1PhDwpLska7gSXdoineUkYLOQA&e=
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> *****
>
> A colleague of mine asked me this but I'm not familiar with "D" Coverslips.
>
> I thought someone here might know.
>
> For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
> Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
> If you have any recommendations and insights, or other suppliers to consider, I'm all ears.
>
>
> Sincerely,
>
> Claire

________________________________

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Lisa Cameron

Re: Coverslips for Primary Neuronal Cultures

In reply to this post by sparker

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Post images on http://www.imgur.com and include the link in your posting.
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The Carolina Biological Supply link is to No 1 (too thin for most
objectives, or at least those noting they are designed to image through
170um thick glass). I've noticed this about a couple other labs' usage of
specific coverslips for neuronal cultures. Perhaps some precedent was set a
long time ago?

Dan (of Bioptechs), do you have No 1.5 coverslips of this D263 glass? I
have labs that want 12mm circles, but I also have an application for 25mm
circles.

By the way, I'm interested myself for info for people who use my core
facility (separate from the original post).

Thanks for your feedback,
Lisa

[hidden email]
Director of Light Microscopy Core Facility, Duke

On Mon, Jan 20, 2020 at 6:04 PM Parker, Sara - (saraparker) <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Everyone,
>
> I use coverglass from Carolina Biological Supply for my primary neuron
> cultures, coated in poly-L-lysine. According to their site, it’s the "the
> famous Deutsche Spiegelglas.” Maybe the D stands for Germany :) ! Good
> luck!
> https://www.carolina.com/catalog/detail.jsp?prodId=633037&s_cid=ppc_gl_products&utm_source=google&utm_medium=cpc&scid=scplp633037&sc_intid=633037&gclid=CjwKCAjwnrjrBRAMEiwAXsCc48U2hkpOztMo9SuwpAQWeg1rqXsF8Hye8tA_TgnKua9gIfHuswOSBhoCFEIQAvD_BwE
>
> Sara Parker
> Postdoctoral Fellow, Mouneimne Lab
> (520) 626-3860
> [hidden email]
> Department of Cellular & Molecular Medicine
> University of Arizona
> On Jan 20, 2020, 2:09 PM -0700, Julia Edgar <[hidden email]>,
> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Claire
> I can't answer you actual question, but this information might still be
> helpful.
> In my experience, neurons adhere much better to glass coverslips coated
> with poly-L-lysine in boric acid buffer versus poly-L-lysine in water. The
> difference is remarkable.
> Best wishes
> Julia
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Claire Brown, Dr.
> Sent: 20 January 2020 20:59
> To: [hidden email]
> Subject: Coverslips for Primary Neuronal Cultures
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> A colleague of mine asked me this but I'm not familiar with "D" Coverslips.
>
> I thought someone here might know.
>
> For microscopy with our primary neuron cultures, we have successfully used
> "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade
> now, but the product is now discontinued. Historically, D coverslips have
> been recommended for neurons because neurons adhere and grow better on them.
> Am I correct that the "D" refers to a particular type of German glass,
> Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and
> Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D"
> refer to "desag", in that case I'm not sure I've found appropriate
> equivalents.
> If you have any recommendations and insights, or other suppliers to
> consider, I'm all ears.
>
>
> Sincerely,
>
> Claire
>

Sylvie Le Guyader

Re: Coverslips for Primary Neuronal Cultures

In reply to this post by Cammer, Michael