Cytometry and Microscopy Symposium Toronto ON November 24-26, 2011

[Claire Brown]

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Abstract deadline is Monday October 31!

Molecular Devices is offering three prizes of $250 for the three top abstracts!

Abstracts will be chosen for symposium talks!

The detailed program is below. We have twelve workshops for you to choose from on Saturday the 26th. Something for everyone.

3rd Bi-Annual Canadian Cytometry and Microscopy Symposium

It will be held at the Delta Chelsea Hotel<http://accounts.deltahotels.com/group.html?uid=G1305037275&type=grp> in the heart of downtown Toronto from November 24 to 26, 2011. I want to extend an invitation to you to attend our symposium.

We have a wonderful program planned (see below for more details). There will be a core facility managers meeting on Thursday afternoon. Scientific symposia all day Friday and there will be a chance that your abstract will be selected for a symposium talk! Saturday is a full day with twelve practical instructional tutorials filled with tips and advice to streamline your research.

The highlight the meeting will be our Thursday night keynote talk by Dr. David Hedley (Ontario Cancer Institute / Princess Margaret Hospital) on Rediscovering cytometry in human cancer biology and advanced therapeutics. The keynote will be followed by a complementary opening reception.

There will be exhibits and poster sessions and lots of time to network, talk to colleagues and talk to vendors. We have worked hard at picking the right venue and getting corporate sponsorship to help fund the event so you will see the registration fees are very reasonable.

Student Registration       $200*
Regular Registration         $350*
*Registration includes the opening reception and two coffee breaks and lunch on Friday and Saturday.

Please go to our web site for more information and to register.

Cytometry and Microscopy in Toronto<http://www.cytometry.ca/en/events/symposium2011/index.html>

Hope to see some of you in Toronto in November!

Claire Brown
CCA Vice-President
McGill Imaging Facility Director

Core Facility Manager meeting
Thursday November 24
Part I (13:00 to 15:00)

  1.  1. Association of Biomolecular Resource Facilities (ABRF)

Overview of the association, extended resources and ways the association helps core facilities in a wide range of scientific specialties.
Elke Küster-Schöck (McGill University/ABRF representative)

  1.  Funding problems and strategies

Overview of current funding situation. How can we as members of the ACC-CCA push for funding solutions in the future?
Kevin Hamilton (Strategic research programs director, Sunnybrook Research Institute)

Coffee Break

Part II (15:30 to 17:30)

  1.  Round Table Discussion
     *   Maintenance contract or not? That is the question. Service contracts - overview of issues. Role the ACC-CCA can play in negotiating reasonable contracts with companies.
     *   Network software tools. Role the ACC-CCA can play in network software licenses.
     *   Round Table Discussion on 5 topics voted during our survey.
  2.  Case scenarios

Presentation of "real life" case scenarios related to core facilities.

Keynote Address

19:00 to 20:00
David Hedley (Ontario Cancer Institute / Princess Margaret Hospital)
Rediscovering cytometry in human cancer biology and advanced therapeutics

20:00 to 22:00
Opening Reception - All Registrants are Welcome

Plenary Sessions
Friday November 25

  1.  Developmental biology (08:30 to 10:00)
     *   Annie Bourdeau (Sunnybrook Health Sciences Centre, Toronto)
Human and mouse studies facilitated by phosflow and cytokine bead arrays.
     *   Julie Yuan (Hospital for Sick Children Research Institute, Toronto)
AML-discovery via phospho-flow and proteomics.
     *   2 abstract presentations

Coffee Break

  1.  Cell biology (10:30 to 12:00)
     *   Sergio Grinstein (Hospital for Sick Children Research Institute, Toronto)
Imaging phagocytosis: receptors, signal transduction and the cytoskeleton
     *   He Song Sun (University of Toronto at Scarborough)
Effects of chlamydia infection on host cell division.
     *   2 abstract presentations

Lunch 12:00 to 14:00 - Poster session - Exhibitors

Lunch and Learn - Sponsored by Lumen Dynamics Grab your boxed lunch and hear about the latest LED light source available for wide-field microscopy.

  1.  Neurology (14:00 to 15:30)
     *   Isabelle Aubert (Sunnybrook Health Sciences Centre, Toronto)
Imaging brain matters
     *   Sheila Singh (Stem Cell and Cancer Research Institute, McMaster University)
Brain tumour initiating cells: why the cancer stem cell hypothesis matters to patients with brain tumours.
     *   Suzie Dufour (Institute of Biomaterials and Biomedical Engineering, University of Toronto)
A microprobe for in vivo single neuron electrophysiology and optogenetics.
     *   1 abstract presentation

Coffee Break

  1.  Chronic diseases (16:00 to 17:30)
     *   Rupert Kaul (Toronto General Research Institute, Toronto)
The immunology of HIV transmission
     *   Laurie Ailles (University Health Network, Toronto)
Using flow cytometry to understand intra- and inter-tumoral heterogeneity in human cancer samples.
     *   Janet Markle (Hospital for Sick Children, University of Toronto)
Immunogenetics of type I diabetes.
     *   1 abstract presentation

Tutorials
Saturday November 26
Morning Tutorial Session - Part I (8:30 to 10:00)

  1.  Multicolor
     *   Laurence Lejeune (Hôpital St-Luc, Université de Montréal)
Introduction to Multicolour Flow Applications
     *   Ian Bates (Carl Zeiss Canada)
Spectral Unmixing in Microscopy
     *   1 abstract presentation
  2.  Live Cells
     *   Judith Lacoste (McGill University)
Live Cell Microscopy: Tips and Tools
     *   Kevin Conway (Nikon Canada)
TBD
     *   Kimberly Young (McGill University)
Nuclear probes for live cell microscopy
     *   George Sakellaropoulos (Olympus Canada and Andor Technology)
Confocal Imaging of Live Cells:  Systems Optimized for High-Speed and Long Time-Course Studies.
  3.  Cell Cycle and apoptosis
     *   Aleks Spurmanis (McGill University)
Old versus new: stains and dyes for cell kinetics
     *   Elizabeth Roquemore (GE Healthcare)
Advantages of High Content Analysis for cell cycle, cell health and cytotoxicity applications.
     *   Bruce Bagwell (Verity Software House)
Gemstone
  4.  Instrument Standardization
     *   Marie-Helene Lacombe (McGill University)
Validating instrument performance in flow cytometry.
     *   Julie Yuan (Hospital for Sick Kids - UHN)
Standardizing multiple analyzers for uniform user results.
     *   Claire Brown (McGill University)
ABRF Light Microscopy Group Standardization Initiatives
     *   Tushare Jinadasa (McGill University)
Confocal Resolution and Quality Control

Coffee Break

Morning Tutorial Session - Part II (10:30 to 12:00)

  1.  Cell Sorting : Applications + Biosafety
     *   Gisele Knowles (Sunnybrook Research Institute)
Overview of specific cell sorting applications: How to sort cells that don't want to be sorted - neurons, tumor cells for starters
     *   Dionne White (University of Toronto)
Biohazard ratings of potential pathogens in cells.
     *   Daniel Sirk (Beckman-Coulter)
Creating the model biosafety sorter: the development of the Beckman-Colter Astrios.
  2.  Light Sources & Detectors
     *   Claire Brown (McGill University)
Microscopy light sources.
     *   Vince Varallo (Olympus Canada and Photometrics)
CMOS, CCD and EMCCD cameras.
     *   Etienne Lareau (Nüvü Cameras)
EMCCD cameras : new developments and applications.
     *   Judith Lacoste (McGill University)
Microscopy detectors: noise and performance
  3.  Advanced Techniques
     *   TBD
Cytof and Applications
     *   James Jonkman (Advanced Optical Microscopy Facility (AOMF), University Health Network)
What the FRAP?! An introduction to advanced photobleaching and photoactivation microscopy
  4.  Introduction to Image Processing and Analysis
     *   Gabriel Lapointe (Concordia University)
Introduction to image processing and analysis.
     *   Tony Collins (McMaster University)
An introduction to multivariate image analysis Case study: an improved apoptosis assay for high content screening.
     *   Pauline Meager (CYTOO)
Reproducible and quantitative analysis of cell morphology and subcellular structures by controlling cell-surface adhesions

Lunch 12:00 to 14:00 - Poster session - Exhibitors

Afternoon Advanced Tutorials (14:00 to 17:30)

  1.  3D Imaging Techniques
     *   Judith Lacoste (McGill University)
Overview of available 3D imaging platforms.
     *   Vincent Schoonderwoert (Scientific Volume Imaging)
Improving image resolution and signal to noise with deconvolution.
     *   Jeff Butler (Quorum Technologies)
3-D image acquisition - advantages for observation and analysis.
     *   Andrew Millar (Olympus Canada)
Advances in Deep 3D Imaging with Multi-photon Excitation
     *   Ralph S. DaCosta (Ontario Cancer Institute, University Health Network)
Intravital 3D Optical Imaging in Preclinical Cancer Research.
     *   Cory Glowinski (Bitplane Inc.)
3D Imaging Processing and Analysis
  2.  Data analysis for Flow cytometry
     *   Ryan Brickman (British Columbia Cancer Research Centre)
Gating is not enough: automate and let go.
     *   Laurence Lejeune (Collaboration with Cytobank)
New concepts in sharing and analyzing data.
     *   Isaiah Henkel (Treestar)
Flow Jo Version 10 and Fluorish Panel Wizardry
     *   TBD
  3.  Measuring Protein-Protein Interactions
     *   Aleks Spurmanis (McGill University)
What do co-localization measurements tell you?
     *   Cecile Fradin (McMaster University)
Fluorescence Correlation Spectroscopy (FCCS)
     *   Claire Brown (McGill University)
Raster Imaging Correlation Spectroscopy (RICS)
     *   Claire Brown (McGill University)
FRET Microscopy - Which technique should I use?
     *   Tony Collins (McMaster University)
Practical FLIM-FRET for cell biologists.
     *   1 abstract presentation
  4.  Advanced Multicolor Applications for Flow
     *   David Ehman (BD Biosciences)
Multi-colour flow: the promise and the reality.
     *   Michele Anderson(University of Toronto)
Needles in a haystack: finding dendritic cells and harvesting them
     *   Sasan Zandi (UHN-Stem Cell Network)
Tracking human stem cell progenitors with FACS
     *   Aleks Spurmanis (McGill University)
Introduction to fluorescence bar coding.
     *   Howard Shapiro (by video conference)
Building an Imaging System Using a Flow Cytometer