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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalI am trying to visualize cell nuclei with DAPI in corn leaf mesophyll cells and
have been having some difficulty. I was wondering if anyone has experience
or tricks doing so.
The samples are corn leaf sections fixed with formaldehyde, embedded in OCT
for cryosectioning, and mounted with VectaShield containing DAPI after OCT is
washed off. I have not been able to see any DAPI staining in the corn
samples, whereas Arabidopsis leaf processed exactly the same way at the
same time shows beautiful DAPI staining. One other detail that is relevant is
that I use the two-photon setting in a Zeiss LSM510 confocal.
Any thoughts or suggestions would be appreciated.
Best regards,
Meng
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