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Hello,
I have a question concerning dihydroethidium (DHE). My friend, who is not a list member, is working on evaluating ROS production in earthworm ovary cells. She is using DHE, which in contact witch ROS transforms to ethidium and migrates to nucleus. We are now wondering if it is possible to evaluate ROS amount by the intensity of nuclear fluorescence, or, as it seems to me, we should find another nuclear stain to be able to differentiate between fluorescence intensity caused by DNA condensation (or amount f NA) and that caused by ROS production.
We will be very grateful for any advise.
Best regards,
Hanna Sas Nowosielska
Department of Plant Anatomy and Cytology
Faculty of Biology and Environmental Protection
University of Silesia
JagielloĊska Str. 28
40 - 032 Katowice
Poland
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