DIC or bright field images on LSM 700

[Atul Anand]

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Dear All,

I am struggling to capture DIC or bright field images our LSM 700 microscopy (ZEN software).
Please help and suggest for this.
Thanks in advance.

Have a nice day

Best Regards
Atul

Venlig hilsen
********************************************
Atul Anand
PhD student
Centre for Autophagy, Recycling and Disease (CARD)
Cell Death and Metabolism
Danish Cancer Society Research Center
Strandboulevarden 49
DK-2100 Copenhagen
Denmark
Tel: [phone]

[weber]

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Hi,

You should have a transmission PMT ?  T-PMT
Just clic on it !

Regards

[Göran Månsson]

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Dear Atul,

Apart from activating the detector for transmitted light (T-PMT) you may
also have to manually switch the light path from the halogen light source
(to see transmitted channel in the eye pieces) to the T-PMT. (This is in
case you have a manual system).

For good quality transmitted channel you must of course set the Kohler
illumination properly.

Regards
Göran

-----Ursprungligt meddelande-----
Från: Confocal Microscopy List [mailto:[hidden email]] För
Atul Anand
Skickat: den 12 februari 2015 10:37
Till: [hidden email]
Ämne: DIC or bright field images on LSM 700

*****
To join, leave or search the confocal microscopy listserv, go to:
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Dear All,

I am struggling to capture DIC or bright field images our LSM 700 microscopy
(ZEN software).
Please help and suggest for this.
Thanks in advance.

Have a nice day

Best Regards
Atul

Venlig hilsen
********************************************
Atul Anand
PhD student
Centre for Autophagy, Recycling and Disease (CARD) Cell Death and Metabolism
Danish Cancer Society Research Center Strandboulevarden 49
DK-2100 Copenhagen
Denmark
Tel: [phone]

[Vickie Frohlich]

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Are you certain that you have the polarizer in the condenser set to the proper position for imaging DIC via laser illumination.  In the inverted scopes the proper position is 90 degrees off form that used for viewing by eye.
Vickie

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Göran Månsson
Sent: Thursday, February 12, 2015 6:50 AM
To: [hidden email]
Subject: SV: DIC or bright field images on LSM 700

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Dear Atul,

Apart from activating the detector for transmitted light (T-PMT) you may also have to manually switch the light path from the halogen light source (to see transmitted channel in the eye pieces) to the T-PMT. (This is in case you have a manual system).

For good quality transmitted channel you must of course set the Kohler illumination properly.

Regards
Göran

-----Ursprungligt meddelande-----
Från: Confocal Microscopy List [mailto:[hidden email]] För Atul Anand
Skickat: den 12 februari 2015 10:37
Till: [hidden email]
Ämne: DIC or bright field images on LSM 700

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear All,

I am struggling to capture DIC or bright field images our LSM 700 microscopy (ZEN software).
Please help and suggest for this.
Thanks in advance.

Have a nice day

Best Regards
Atul

Venlig hilsen
********************************************
Atul Anand
PhD student
Centre for Autophagy, Recycling and Disease (CARD) Cell Death and Metabolism Danish Cancer Society Research Center Strandboulevarden 49
DK-2100 Copenhagen
Denmark
Tel: [phone]