Deconvolution Microscope Freeware

classic Classic list List threaded Threaded
6 messages Options
Heather Bowden Heather Bowden
Reply | Threaded
Open this post in threaded view
|

Deconvolution Microscope Freeware

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I have a Deltavision deconvolution microscope. All components are from 2001
and the unit was donated to us. The software upgrade costs an arm and a leg
and we are a nonprofit. Can anyone please send me some links to freeware
that they recommend? Thanks!
Kolja Wawrowsky Kolja Wawrowsky
Reply | Threaded
Open this post in threaded view
|

Re: Deconvolution Microscope Freeware

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I like the "Deconvolution Lab" of the Biomedical Imaging Group at the École polytechnique fédérale de Lausanne. It is a free ImageJ plug-in.
 
link:
http://bigwww.epfl.ch/algorithms/deconvolution/

Good luck,
Kolja

_____________________________
Kolja Wawrowsky, PhD
Director Light Microscopy Core
Cedars-Sinai Medical Center

8700 Beverly Blvd
Davis 6068
Los Angeles, CA 90048

ph: 310-849-5291
lab: 310-423-4429

e-mail: [hidden email]


On Dec 5, 2012, at 4:12 PM, Heather Bowden <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I have a Deltavision deconvolution microscope. All components are from 2001
> and the unit was donated to us. The software upgrade costs an arm and a leg
> and we are a nonprofit. Can anyone please send me some links to freeware
> that they recommend? Thanks!
Paul Herzmark Paul Herzmark
Reply | Threaded
Open this post in threaded view
|

Re: Deconvolution Microscope Freeware

In reply to this post by Heather Bowden
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Heather,
Do you also need microscope control software to move the stage and operate
the camera etc? In that case you should check out micromanager.

www.*micro-manager*.org/

It is free and runs in ImageJ.

Paul

Paul Herzmark
Specialist
[hidden email]

Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA  94720-3200
(510) 643-9603
(510) 643-9500 fax


On Wed, Dec 5, 2012 at 4:12 PM, Heather Bowden <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I have a Deltavision deconvolution microscope. All components are from 2001
> and the unit was donated to us. The software upgrade costs an arm and a leg
> and we are a nonprofit. Can anyone please send me some links to freeware
> that they recommend? Thanks!
>
Guy Cox-2 Guy Cox-2
Reply | Threaded
Open this post in threaded view
|

Re: Deconvolution Microscope Freeware

In reply to this post by Heather Bowden
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Heather,

               You do not mention why you want to update the software.  If you install freeware you will just have a fairly routine Olympus microscope instead of the tightly-integrated Deltavision system.   Assuming that you are not upgrading hardware, and it doesn't sound like you are, the 2001 software will do everything you need.  Deconvolution algorithms haven't changed much in that time.   Sure, you will be running an out-of-date OS but why does that matter in a single-purpose system?

                                                Guy



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Heather Bowden
Sent: Thursday, 6 December 2012 11:13 AM
To: [hidden email]
Subject: Deconvolution Microscope Freeware

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I have a Deltavision deconvolution microscope. All components are from 2001 and the unit was donated to us. The software upgrade costs an arm and a leg and we are a nonprofit. Can anyone please send me some links to freeware that they recommend? Thanks!
G. Esteban Fernandez G. Esteban Fernandez
Reply | Threaded
Open this post in threaded view
|

Re: Deconvolution Microscope Freeware

In reply to this post by Heather Bowden
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Heather,

Paul's suggestion of Micro-Manager may be a good option.  Maybe
someone who has tried to control a Deltavision with MM will chime in
but you can check out the "Devices" page at
http://www.micro-manager.org to see if it can control your components.
 Is your Deltavision composed from hardware (stage, camera,
microscope, filter wheels, etc.) of known brands/models?

-Esteban


On 12/5/12, Heather Bowden <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I have a Deltavision deconvolution microscope. All components are from 2001
> and the unit was donated to us. The software upgrade costs an arm and a leg
> and we are a nonprofit. Can anyone please send me some links to freeware
> that they recommend? Thanks!
>
Heather Bowden Heather Bowden
Reply | Threaded
Open this post in threaded view
|

Re: Deconvolution Microscope Freeware

In reply to this post by Guy Cox-2
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Thank you for your suggestions.

There is a problem with the microscope software (SoftWorx). It will not
sync to any part of the microscope. The hardware works fine. API wants
$4,500 to attempt to fix the software. They emphasize that the entire
system is outdated (even the microscope stand). They encourage me to buy an
entire new system ($130K).

The unit itself is assembled with parts from API, although I don't know the
source of each part. It was donated and did not come with a parts list. But
I could look up the individual components. However, it's the software that
is the problem. I see that deconvolution can be performed with
DeconvolutionLab. But this doesn't help with the driver. Post-donation, the
whole thing was also assembled and tested by API. They insist that it
worked after installation...

Does anyone have a not-too-old confocal they would like to donate to a
non-profit? :)

Thank you,

Heather

On Wed, Dec 5, 2012 at 9:23 PM, Guy Cox <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Heather,
>
>                You do not mention why you want to update the software.  If
> you install freeware you will just have a fairly routine Olympus microscope
> instead of the tightly-integrated Deltavision system.   Assuming that you
> are not upgrading hardware, and it doesn't sound like you are, the 2001
> software will do everything you need.  Deconvolution algorithms haven't
> changed much in that time.   Sure, you will be running an out-of-date OS
> but why does that matter in a single-purpose system?
>
>                                                 Guy
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Heather Bowden
> Sent: Thursday, 6 December 2012 11:13 AM
> To: [hidden email]
> Subject: Deconvolution Microscope Freeware
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I have a Deltavision deconvolution microscope. All components are from
> 2001 and the unit was donated to us. The software upgrade costs an arm and
> a leg and we are a nonprofit. Can anyone please send me some links to
> freeware that they recommend? Thanks!
>



--
----------------------------------------------------------



E: [hidden email]
GoogleVoice: 408-840-3643
http://www.arcbio.com