Benjamin Smith |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I had a question about commercial/open-source image acquisition software. Considering that on most scientific equipment intensity is measured on a linear scale (on a good day), does the corresponding image software show that same data rendered with a default 0.45 gamma to offset the 2.2 gamma in the monitor (i.e. render the raw data as it would appear to the eye), or do they simply leave in the 2.2 gamma leading to over contrasted images as the default setting? I searched and found a thread on the listserv from 2017 about this, but only in regards to cameras and monitors as opposed to the software. In this case, I'm more interested in the software and what the "industry standard" is for displaying linear scale image data on computer monitors. Thanks for any insights, Ben Smith -- Benjamin E. Smith, Ph. D. Imaging Specialist, Vision Science University of California, Berkeley 195 Life Sciences Addition Berkeley, CA 94720-3200 Tel (510) 642-9712 Fax (510) 643-6791 e-mail: [hidden email] http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/> |
Michael Giacomelli-2 |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** My anecdotal experience is that most software made to work with RGB cameras compensates for gamma (since colors look wrong otherwise), and most software that uses monochrome data or point scanning does not. I haven't used very much commercial software however. Mike On Mon, May 13, 2019 at 5:47 PM Benjamin Smith <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=kbmfwr1Yojg42sGEpaQh5ofMHBeTl9EI2eaqQZhHbOU&r=0LyF_z8oU1XGGyisIeOIXyIGIM5IYb3NcLjxHjUca5Y&m=baxCYO7OSnI25affkFspBVg57dzN_vyxDlr6HK-3ePU&s=M9r9B4V6aAxmmHFTkTAG9M4GwhhqgM1kW5VvnDnEoNA&e= > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=kbmfwr1Yojg42sGEpaQh5ofMHBeTl9EI2eaqQZhHbOU&r=0LyF_z8oU1XGGyisIeOIXyIGIM5IYb3NcLjxHjUca5Y&m=baxCYO7OSnI25affkFspBVg57dzN_vyxDlr6HK-3ePU&s=R3dCXuNespjLq3E39pN4UXPS-7Cj240Ca8pRKldkNqk&e= and include the link in your posting. > ***** > > I had a question about commercial/open-source image acquisition software. > Considering that on most scientific equipment intensity is measured on a > linear scale (on a good day), does the corresponding image software show > that same data rendered with a default 0.45 gamma to offset the 2.2 gamma > in the monitor (i.e. render the raw data as it would appear to the eye), or > do they simply leave in the 2.2 gamma leading to over contrasted images as > the default setting? > > I searched and found a thread on the listserv from 2017 about this, but > only in regards to cameras and monitors as opposed to the software. In > this case, I'm more interested in the software and what the "industry > standard" is for displaying linear scale image data on computer monitors. > > Thanks for any insights, > Ben Smith > > -- > Benjamin E. Smith, Ph. D. > Imaging Specialist, Vision Science > University of California, Berkeley > 195 Life Sciences Addition > Berkeley, CA 94720-3200 > Tel (510) 642-9712 > Fax (510) 643-6791 > e-mail: [hidden email] > https://urldefense.proofpoint.com/v2/url?u=http-3A__vision.berkeley.edu_-3Fpage-5Fid-3D5635&d=DwIBaQ&c=kbmfwr1Yojg42sGEpaQh5ofMHBeTl9EI2eaqQZhHbOU&r=0LyF_z8oU1XGGyisIeOIXyIGIM5IYb3NcLjxHjUca5Y&m=baxCYO7OSnI25affkFspBVg57dzN_vyxDlr6HK-3ePU&s=VbucRuauI1QcBU99BEE8NQh_G_8lQmclFKd00BPfjXU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__vision.berkeley.edu_&d=DwIBaQ&c=kbmfwr1Yojg42sGEpaQh5ofMHBeTl9EI2eaqQZhHbOU&r=0LyF_z8oU1XGGyisIeOIXyIGIM5IYb3NcLjxHjUca5Y&m=baxCYO7OSnI25affkFspBVg57dzN_vyxDlr6HK-3ePU&s=MiFiKCYgYyK4p3W4m0C3u3T-DpUmOLkjtNN5hHTEOeQ&e=> |
Zdenek Svindrych-2 |
In reply to this post by Benjamin Smith
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Ben, a quick test with this image: http://www.lagom.nl/lcd-test/gamma_calibration.php on my laptop (LCD IPS screen) show that my browser corrects gamma to some extent (values about 1.2 - 1.5), but the same test image opened in ImageJ shows gamma around 2.2, that means no correction. You can try for yourself with whatever commercial software you're using (and let us know if you find something interesting:-). Best, zdenek ---------- Zdenek Svindrych, Ph.D. Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth On Mon, May 13, 2019 at 5:47 PM Benjamin Smith <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > I had a question about commercial/open-source image acquisition software. > Considering that on most scientific equipment intensity is measured on a > linear scale (on a good day), does the corresponding image software show > that same data rendered with a default 0.45 gamma to offset the 2.2 gamma > in the monitor (i.e. render the raw data as it would appear to the eye), or > do they simply leave in the 2.2 gamma leading to over contrasted images as > the default setting? > > I searched and found a thread on the listserv from 2017 about this, but > only in regards to cameras and monitors as opposed to the software. In > this case, I'm more interested in the software and what the "industry > standard" is for displaying linear scale image data on computer monitors. > > Thanks for any insights, > Ben Smith > > -- > Benjamin E. Smith, Ph. D. > Imaging Specialist, Vision Science > University of California, Berkeley > 195 Life Sciences Addition > Berkeley, CA 94720-3200 > Tel (510) 642-9712 > Fax (510) 643-6791 > e-mail: [hidden email] > http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/> > -- -- Zdenek Svindrych, Ph.D. Research Associate - Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth |
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