Dehydroergosterol (DHE) multiphoton imaging in plants?

[Stanislav Vitha-2]

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Hallo,
one of our facility users wants to do multiphoton imaging of the fluorescent
choleosterol analog Dehydroergosterol (DHE)  in plants (Arabidopsis).  I
have only seen studies with cultured animal cells.

Have any of you tried this in plants?   Please share hints and success or
failure stories.

1-P Absorption is around 330 nm,  emission ~370 to 430 nm.
It looks like 3-photon excitation  is normally used - laser tuned to 920 nm
and used at fairly high power, 70 mW.
 

Thanks in advance!

Stan Vitha
Texas A&M University
Microscopy and Imaging Center

[Nuno Moreno]

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Hi,

I would try first something broader dye like DI4 AnnepD HQ. I'm not aware of any dye with that specificity for live imaging. Let me know if you succeed. I've used already in plants.

http://www.cell.com/biophysj/abstract/S0006-3495(06)72437-7

NM




On 25 Jun 2014, at 15:47, Stanislav Vitha <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hallo,
> one of our facility users wants to do multiphoton imaging of the fluorescent
> choleosterol analog Dehydroergosterol (DHE)  in plants (Arabidopsis).  I
> have only seen studies with cultured animal cells.
>
> Have any of you tried this in plants?   Please share hints and success or
> failure stories.
>
> 1-P Absorption is around 330 nm,  emission ~370 to 430 nm.
> It looks like 3-photon excitation  is normally used - laser tuned to 920 nm
> and used at fairly high power, 70 mW.
>
>
> Thanks in advance!
>
> Stan Vitha
> Texas A&M University
> Microscopy and Imaging Center