Detect red kaede in fixed tissues

[Claudia Campos]

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Hi!
Anybody knows about a good strategy to fix the red kaede several hours
after photoconversion in a way that it still stays bright to be found in
a fixed fish embryo? Right now when I fix it, the fluorescence decreases
80% and it makes it extremely hard to find a photoconverted cell in the
middle of the whole animal.

Thanks

Claudia
Gonzalez lab
University of Geneva

[Simon Ameer-Beg]

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Dear List,

Does anyone have a reference for the 2-photon cross-section for the dye Badan?

Any information greatly appreciated

Best wishes

Simon


Dr Simon M. Ameer-Beg

Non-clinical lecturer
The Richard Dimbleby Department of Cancer Research
Randall Division of Cell & Molecular Biophysics and Division of Cancer Studies
King's College London
2rd Floor, Room 2.30A
New Hunt's House, Guy's Medical School Campus
London SE1 1UL