DiasproLab at DIFILAB and Nanoscopy IIT Claudio Canale research

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Alberto Diaspro-2 Alberto Diaspro-2
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DiasproLab at DIFILAB and Nanoscopy IIT Claudio Canale research

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Dear,
I think this is a key work for those working on fluorescence nanoscopy. What are we really labellin?
Best
Alby

link: https://advances.sciencemag.org/content/5/6/eaav8062.full

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https://loop.frontiersin.org/people/106070/overview
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Vitaly Boyko-2 Vitaly Boyko-2
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Re: DiasproLab at DIFILAB and Nanoscopy IIT Claudio Canale research

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 Hi Alberto,
Very expected result!Most of the articles published on fluorescently labeled biological molecules do not include functional assays on wildtype versus tagged molecules (assuming that FP::protein/ligand fusions behave similarly to a wild type molecule; there are rarely protein attenuation assay included or developed; as its development is often another 3 year project difficult to get funding for). Thus, tentative conclusion: 21st century biology is still "swimming" in over expression artefacts and/or chronic lack of function or attenuation assays???
Cheers,
Vitaly

    On Wednesday, June 26, 2019, 03:36:23 PM EDT, Alberto Diaspro <[hidden email]> wrote:  
 
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Dear,
I think this is a key work for those working on fluorescence nanoscopy. What are we really labellin?
Best
Alby

link: https://advances.sciencemag.org/content/5/6/eaav8062.full

---------
https://loop.frontiersin.org/people/106070/overview
---------  
Vitaly Boyko-2 Vitaly Boyko-2
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Re: DiasproLab at DIFILAB and Nanoscopy IIT Claudio Canale research

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 Totally agree, and being careful when interpreting the data
    On Wednesday, June 26, 2019, 03:36:23 PM EDT, Alberto Diaspro <[hidden email]> wrote:  
 
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Dear,
I think this is a key work for those working on fluorescence nanoscopy. What are we really labellin?
Best
Alby

link: https://advances.sciencemag.org/content/5/6/eaav8062.full

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https://loop.frontiersin.org/people/106070/overview
---------  
maiti maiti
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Re: DiasproLab at DIFILAB and Nanoscopy IIT Claudio Canale research

In reply to this post by Alberto Diaspro-2
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Dear Alby,
This is truly intetesting to us, as we are
working on this area. We have made a
similar instrument, so will  check these
results for our samples right away.
Thanks!
Sudipta

On Wed, 26 Jun 2019 21:35:47 +0200,
Alberto Diaspro wrote
> *****
> To join, leave or search the confocal
microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?
A0=confocalmicroscopy
> Post images on http://www.imgur.com and
include the link in your posting.
> *****
>
> Dear,
> I think this is a key work for those
working on fluorescence
> nanoscopy. What are we really labellin?
Best Alby
>
> link:
https://advances.sciencemag.org/content/5/
6/eaav8062.full
>
> ---------
>
https://loop.frontiersin.org/people/106070
/overview
> ---------


Prof. Sudipta Maiti
Dept. of Chemical Sciences
Tata Institute of Fundamental Research
Homi Bhabha Road, Colaba
Mumbai 400005, India
Ph. +91 222 278 2716
Alternate e-mail: [hidden email]
webpage: biophotonics.co.in