Alberto Diaspro-2 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear, I think this is a key work for those working on fluorescence nanoscopy. What are we really labellin? Best Alby link: https://advances.sciencemag.org/content/5/6/eaav8062.full --------- https://loop.frontiersin.org/people/106070/overview --------- |
Vitaly Boyko-2 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Alberto, Very expected result!Most of the articles published on fluorescently labeled biological molecules do not include functional assays on wildtype versus tagged molecules (assuming that FP::protein/ligand fusions behave similarly to a wild type molecule; there are rarely protein attenuation assay included or developed; as its development is often another 3 year project difficult to get funding for). Thus, tentative conclusion: 21st century biology is still "swimming" in over expression artefacts and/or chronic lack of function or attenuation assays??? Cheers, Vitaly On Wednesday, June 26, 2019, 03:36:23 PM EDT, Alberto Diaspro <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear, I think this is a key work for those working on fluorescence nanoscopy. What are we really labellin? Best Alby link: https://advances.sciencemag.org/content/5/6/eaav8062.full --------- https://loop.frontiersin.org/people/106070/overview --------- |
Vitaly Boyko-2 |
In reply to this post by Alberto Diaspro-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Totally agree, and being careful when interpreting the data On Wednesday, June 26, 2019, 03:36:23 PM EDT, Alberto Diaspro <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear, I think this is a key work for those working on fluorescence nanoscopy. What are we really labellin? Best Alby link: https://advances.sciencemag.org/content/5/6/eaav8062.full --------- https://loop.frontiersin.org/people/106070/overview --------- |
In reply to this post by Alberto Diaspro-2
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Alby, This is truly intetesting to us, as we are working on this area. We have made a similar instrument, so will check these results for our samples right away. Thanks! Sudipta On Wed, 26 Jun 2019 21:35:47 +0200, Alberto Diaspro wrote > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa? A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear, > I think this is a key work for those working on fluorescence > nanoscopy. What are we really labellin? Best Alby > > link: https://advances.sciencemag.org/content/5/ 6/eaav8062.full > > --------- > https://loop.frontiersin.org/people/106070 /overview > --------- Prof. Sudipta Maiti Dept. of Chemical Sciences Tata Institute of Fundamental Research Homi Bhabha Road, Colaba Mumbai 400005, India Ph. +91 222 278 2716 Alternate e-mail: [hidden email] webpage: biophotonics.co.in |
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