Dipping Lens Cleaning Protocol

[Claire Brown]

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Does anyone have a good protocol for cleaning dipping lenses?

Sincerely,

Claire

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CANADIAN CYTOMETRY AND MICROSCOPY SYMPOSIUM - MONTREAL JUNE 13-15 - http://www.regonline.com/ccma-accm2013

Claire M. Brown, PhD - McGill University - Assistant Professor, Physiology - Life Sciences Complex Imaging Facility Director
3649 Promenade Sir William Osler - Bellini Building - Rm 137A - Montreal - Quebec - H3G 0B1
514-398-4400 ext 00795 (Phone) - 514-677-7493 (Cell) - 514-398-7452 (FAX)
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[Hotting Lo]

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We use ethanol to clean off the organic solutions. Pretty good.

On Jun 5, 2013, at 5:29 PM, "Claire Brown, Dr." <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Does anyone have a good protocol for cleaning dipping lenses?
>
> Sincerely,
>
> Claire
>
> -------------------------------------------------------------------------------------------
> CANADIAN CYTOMETRY AND MICROSCOPY SYMPOSIUM - MONTREAL JUNE 13-15 - http://www.regonline.com/ccma-accm2013
>
> Claire M. Brown, PhD - McGill University - Assistant Professor, Physiology - Life Sciences Complex Imaging Facility Director
> 3649 Promenade Sir William Osler - Bellini Building - Rm 137A - Montreal - Quebec - H3G 0B1
> 514-398-4400 ext 00795 (Phone) - 514-677-7493 (Cell) - 514-398-7452 (FAX)
> [hidden email]<mailto:[hidden email]> - http://www.mcgill.ca/lifesciencescomplex/core/imaging

[Dmitry Sokolov]

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Hi Claire,

the objectives cleaning instructions can be found here:
http://confocal-manawatu.pbworks.com/w/page/16346952/LM%20Maintenance

Cheers,
Dmitry

*Advanced Knowledge Management*
for *MICROSCOPY *and *Image Analysis *
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*Dmitry Sokolov*, Ph.D.
Mob: *[phone]***
[hidden email] <mailto:[hidden email]>



On 06.06.2013 11:55, Hotting Lo wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We use ethanol to clean off the organic solutions. Pretty good.
>
> On Jun 5, 2013, at 5:29 PM, "Claire Brown, Dr." <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Does anyone have a good protocol for cleaning dipping lenses?
>>
>> Sincerely,
>>
>> Claire
>>
>> -------------------------------------------------------------------------------------------
>> CANADIAN CYTOMETRY AND MICROSCOPY SYMPOSIUM - MONTREAL JUNE 13-15 - http://www.regonline.com/ccma-accm2013
>>
>> Claire M. Brown, PhD - McGill University - Assistant Professor, Physiology - Life Sciences Complex Imaging Facility Director
>> 3649 Promenade Sir William Osler - Bellini Building - Rm 137A - Montreal - Quebec - H3G 0B1
>> 514-398-4400 ext 00795 (Phone) - 514-677-7493 (Cell) - 514-398-7452 (FAX)
>> [hidden email]<mailto:[hidden email]> - http://www.mcgill.ca/lifesciencescomplex/core/imaging

[Steffen Dietzel]

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Most of the time I just pour distilled water over the tip and suck of
the remaining drop with a tissue. If there is real dirt (crystals,
silicone) I use wood sticks with soft, fat free, medical quality cotton
wrapped around and moistened with Water or Methanol. If necessary, I do
this under the stereo microscope.

The is a manual from Zeiss, called the clean microscope (or in German
das saubere Mikroskop) which explains some of the dos and don'ts:
http://www.zeiss.com/C125792900358A3F/0/3C1DAE76330E42F7C12579EC0028F56B/$FILE/50-1-0025_the_clean_microscope_e.pdf

Steffen

On 05.06.2013 22:52, Claire Brown, Dr. wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Does anyone have a good protocol for cleaning dipping lenses?
>
> Sincerely,
>
> Claire
>
> -------------------------------------------------------------------------------------------
> CANADIAN CYTOMETRY AND MICROSCOPY SYMPOSIUM - MONTREAL JUNE 13-15 - http://www.regonline.com/ccma-accm2013
>
> Claire M. Brown, PhD - McGill University - Assistant Professor, Physiology - Life Sciences Complex Imaging Facility Director
> 3649 Promenade Sir William Osler - Bellini Building - Rm 137A - Montreal - Quebec - H3G 0B1
> 514-398-4400 ext 00795 (Phone) - 514-677-7493 (Cell) - 514-398-7452 (FAX)
> [hidden email]<mailto:[hidden email]>  - http://www.mcgill.ca/lifesciencescomplex/core/imaging
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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