Disinfecting/Sterilising water dipping objective

[Martin Lenz]

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Hi everyone,

we have an Olympus 25x water dipping objective and would like to run 5 day or longer time lapse imaging experiments.

After a few days imaging in growth media we clearly see an infection (probably bacterial) with the objective dipped in. A dish next to it, without the objective does not show an infection.
We tried cleaning the objective with EtOH (basically dipping the tip in it) and Virusolve (gentle wipe) and that still does not help.

Does anyone (maybe even someone from Olympus ???) have some tips how to disinfect or sterilise the objective without harming it?

Thanks,
Martin

--
Martin O. Lenz
Cambridge Advanced Imaging Centre
University of Cambridge | Anatomy Building | Downing Site | Cambridge | CB2 3DY | UK

[Zdenek Svindrych-2]

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I was just going to propose Cavicide1... but it's almost the same as
Virusolve.
It's ethanol, isopropanol, water and some quaternary ammonium salt. It's not
corrosive...
I would not be afraid to dip the lens into cavicide for couple minutes and
wash with ethanol and DI water (but make sure not to bring any contaminants
from the objective barrel down to the front lens during washing). No wipes
or tissue.
But check with Olympus first.
Best, zdenek


---------- Původní zpráva ----------
Od: Martin Lenz <[hidden email]>
Komu: [hidden email]
Datum: 25. 1. 2017 12:42:37
Předmět: Disinfecting/Sterilising water dipping objective

"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi everyone,

we have an Olympus 25x water dipping objective and would like to run 5 day
or longer time lapse imaging experiments.

After a few days imaging in growth media we clearly see an infection
(probably bacterial) with the objective dipped in. A dish next to it,
without the objective does not show an infection.
We tried cleaning the objective with EtOH (basically dipping the tip in it)
and Virusolve (gentle wipe) and that still does not help.

Does anyone (maybe even someone from Olympus ???) have some tips how to
disinfect or sterilise the objective without harming it?

Thanks,
Martin

--
Martin O. Lenz
Cambridge Advanced Imaging Centre
University of Cambridge | Anatomy Building | Downing Site | Cambridge | CB2
3DY | UK
"

[Michael Delannoy]

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Zdenek,
Dipping alone will not remove the crud.  Use an cotton applicator stick wrapped in lens paper with your lens cleaner.  It is useful to do this under a dissection microscope.  Be extremely careful of using solvents.

http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artfeb04/cdclean.html

good luck,
Michael Delannoy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email]
Sent: Wednesday, January 25, 2017 1:54 PM
To: [hidden email]
Subject: Re: Disinfecting/Sterilising water dipping objective

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*****

I was just going to propose Cavicide1... but it's almost the same as Virusolve.
It's ethanol, isopropanol, water and some quaternary ammonium salt. It's not corrosive...
I would not be afraid to dip the lens into cavicide for couple minutes and wash with ethanol and DI water (but make sure not to bring any contaminants from the objective barrel down to the front lens during washing). No wipes or tissue.
But check with Olympus first.
Best, zdenek


---------- Původní zpráva ----------
Od: Martin Lenz <[hidden email]>
Komu: [hidden email]
Datum: 25. 1. 2017 12:42:37
Předmět: Disinfecting/Sterilising water dipping objective

"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi everyone,

we have an Olympus 25x water dipping objective and would like to run 5 day or longer time lapse imaging experiments.

After a few days imaging in growth media we clearly see an infection (probably bacterial) with the objective dipped in. A dish next to it, without the objective does not show an infection.
We tried cleaning the objective with EtOH (basically dipping the tip in it) and Virusolve (gentle wipe) and that still does not help.

Does anyone (maybe even someone from Olympus ???) have some tips how to disinfect or sterilise the objective without harming it?

Thanks,
Martin

--
Martin O. Lenz
Cambridge Advanced Imaging Centre
University of Cambridge | Anatomy Building | Downing Site | Cambridge | CB2 3DY | UK "
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