EMCCD for speckle TIRFM

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David Spafford David Spafford
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EMCCD for speckle TIRFM

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Hi,

I am moving into the world of speckle, objective-based TIRFM to track
membrane proteins in developing growth cones.  I am trying to figure out what
kind of EMCCD camera to purchase.  I am trying to work through what are the
important specs on the different highest end cameras such as signal to noise,
sensitivity, pixel sizes, exposure times.

Roper Photometrics Cascade II 1024B
Andor Ixon EM+
Hamamatsu EM-CCD C9100-02

Any experiences with these cameras would be appreciated.  Thanks,

David Spafford,
Department of Biology, University of Waterloo
200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1
Rietdorf, Jens Rietdorf, Jens
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Re: EMCCD for speckle TIRFM

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Dear David,

Singe molecule TIR imaging is done with a Cascade II:512B in one of the
groups at embl.

Contact me off line for more detail if required.

Cheers, jens


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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of David Spafford
Sent: Samstag, 12. April 2008 04:48
To: [hidden email]
Subject: EMCCD for speckle TIRFM

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi,

I am moving into the world of speckle, objective-based TIRFM to track
membrane proteins in developing growth cones.  I am trying to figure out
what kind of EMCCD camera to purchase.  I am trying to work through what
are the important specs on the different highest end cameras such as
signal to noise, sensitivity, pixel sizes, exposure times.

Roper Photometrics Cascade II 1024B
Andor Ixon EM+
Hamamatsu EM-CCD C9100-02

Any experiences with these cameras would be appreciated.  Thanks,

David Spafford,
Department of Biology, University of Waterloo 200 University Avenue
West, Waterloo, Ontario, Canada N2L 3G1