Electrical stimulation experiments on confocal

[Konstantín Levitskiy]

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Dear colleagues.
We are now in the process of purchasing a new confocal system. We have practically already defined the technical characteristics of the confocal that we need. However, electrical stimulation would do us great for some perfusion experiments.
Confocal representatives have already adjusted to maximum available what we can spend on the purchase of this equipment to give us all the technology required for our Centre.
But if we want to add the possibility of electrical stimulation we would have to lower (detriment) some technical characteristics of the confocal system of our interest to be able to include pieces from "third" company. Please, could you advice some companies and low prices.
By the way, I cannot also understand why Pt electrodes are needed for that, their potential could be change not only when the bath solution is changed, but the potential could be changed including with/without O2 in the dissolved solution. The chloride Ar electrodes I could thing be a better solution.
Best regards and health for everybody,
Konstantin

[Sylvie Le Guyader]

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Hi Konstantin

Not sure what you mean by electric stimulation but we have users who pulse cardiomyocytes with electric pulses on our inverted systems. We got a custom made stage insert from a local workshop. This allows us to plunge electrodes into each well of a 6 well plate and pulse without having to change anything to the design of our system.
As i understand this is quite common in that field. I suggest you look up 'cardiomyocytes pulsing calcium imaging' to find out who can supply you with the electrode part.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
Follow our microscopy blog!

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Konstantin <[hidden email]>
Sent: Tuesday, June 9, 2020 6:33:45 PM
To: [hidden email] <[hidden email]>
Subject: Electrical stimulation experiments on confocal

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Dear colleagues.
We are now in the process of purchasing a new confocal system. We have practically already defined the technical characteristics of the confocal that we need. However, electrical stimulation would do us great for some perfusion experiments.
Confocal representatives have already adjusted to maximum available what we can spend on the purchase of this equipment to give us all the technology required for our Centre.
But if we want to add the possibility of electrical stimulation we would have to lower (detriment) some technical characteristics of the confocal system of our interest to be able to include pieces from "third" company. Please, could you advice some companies and low prices.
By the way, I cannot also understand why Pt electrodes are needed for that, their potential could be change not only when the bath solution is changed, but the potential could be changed including with/without O2 in the dissolved solution. The chloride Ar electrodes I could thing be a better solution.
Best regards and health for everybody,
Konstantin



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[Craig Brideau]

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The big expense is the amplifiers and digital acquisition to catch the I/V
signals, as well as the current/voltage clamp hardware. You can shop around
for those, or maybe even find some 'gently used' hardware at a steep
discount. The probes on the end will of course need to be new, but as
Sylvie says you should be able to modify an existing microscope stage
cheaply to hold everything. The one question is environmental vibration.
Ephys stages are constructed to minimize vibration, and you might not be
able to accurately place your electrodes if the sample moves too much.
'Real' ephys stages are anchored to the table, and the microscope is placed
on a moving platform to ensure that the sample is as stable as possible.
Conversely, a 'normal' microscope is anchored to the table and the stage
itself moves. This moving stage may or may not be stable enough for your
needs. If you are in a basement area, or can otherwise confirm
relatively low environmental vibration (also consider ventilation effects!)
then you might be able to simply modify a classic moving stage and be done
with it. Otherwise you either have to purchase the ultra-stable fixed stage
and moving microscope platform or purchase equipment to mitigate the
vibration/air flow issues.

Craig

On Wed, Jun 10, 2020 at 1:03 AM Sylvie Le Guyader <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Konstantin
>
> Not sure what you mean by electric stimulation but we have users who pulse
> cardiomyocytes with electric pulses on our inverted systems. We got a
> custom made stage insert from a local workshop. This allows us to plunge
> electrodes into each well of a 6 well plate and pulse without having to
> change anything to the design of our system.
> As i understand this is quite common in that field. I suggest you look up
> 'cardiomyocytes pulsing calcium imaging' to find out who can supply you
> with the electrode part.
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Blickagången 16,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website
> Follow our microscopy blog!
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on
> behalf of Konstantin <[hidden email]>
> Sent: Tuesday, June 9, 2020 6:33:45 PM
> To: [hidden email] <[hidden email]>
> Subject: Electrical stimulation experiments on confocal
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C9ca991081a24415f556a08d80c92f21b%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637273172553155911&amp;sdata=EYNLPfcPqbD5R0xIckdCa8yeJUNR4%2BfSY1VnyP3dxfg%3D&amp;reserved=0
> Post images on
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C9ca991081a24415f556a08d80c92f21b%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637273172553155911&amp;sdata=7i3nNnguWMtFaLhsel1p%2Bj2%2FMLnEm65RJogAmTOYMJo%3D&amp;reserved=0
> and include the link in your posting.
> *****
>
> Dear colleagues.
> We are now in the process of purchasing a new confocal system. We have
> practically already defined the technical characteristics of the confocal
> that we need. However, electrical stimulation would do us great for some
> perfusion experiments.
> Confocal representatives have already adjusted to maximum available what
> we can spend on the purchase of this equipment to give us all the
> technology required for our Centre.
> But if we want to add the possibility of electrical stimulation we would
> have to lower (detriment) some technical characteristics of the confocal
> system of our interest to be able to include pieces from "third" company.
> Please, could you advice some companies and low prices.
> By the way, I cannot also understand why Pt electrodes are needed for
> that, their potential could be change not only when the bath solution is
> changed, but the potential could be changed including with/without O2 in
> the dissolved solution. The chloride Ar electrodes I could thing be a
> better solution.
> Best regards and health for everybody,
> Konstantin
>
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI
> kommer att behandla dina personuppgifter. Här finns information om hur KI
> behandlar personuppgifter<
> https://ki.se/medarbetare/integritetsskyddspolicy>.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing
> your personal data. You can read more about KI's processing of personal
> data here<https://ki.se/en/staff/data-protection-policy>.
>