Expansion Microscopy

[Ashley Ferri]

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Good afternoon.  

I am a student at the University of South Carolina School of Medicine working on my Master's thesis; the topic is expansion microscopy (specifically, I'm using the Protein Retention Expansion Microscopy protocol.)  I want to reach out and see if anyone has previously worked with this technique with cardiac tissue, specifically looking at the connexin proteins in intercalated discs?  I have seen references to the MAP technique being used with heart tissue, but not ProExM.​ If so, what has been your experiences (successes and failures)?  Also, does anyone have a good protocol for MAP so that I may be able to look at the differences?  Thank you for any help you can give me.
 
~ Ashley Ferri
Graduate Research Assistant
University of South Carolina School of Medicine
[hidden email]

[Sverre grødem-3]

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Hi Ashley,

I've been doing ProExm for a while now with, usually, great success. I also tried it once on cardiomyocytes, but I believe they were dissociated (?). I don't remember, it was from a colleague who works with hearts who was curious abut the technique. But it worked!

You should contact Ed Boyden through the [hidden email]<mailto:[hidden email]>  ​address, he keeps a list of all proexm papers so I'll bet he can help you. And in my experience you'll get a response immediately.

I would also strongly suggest to just go ahead and test it. The reagents are cheap and in my experience, most amateurs can do it on the first or second try.

Best regards and good luck!

Sverre Grødem,

University of Oslo
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From: Confocal Microscopy List <[hidden email]> on behalf of Ashley Ferri <[hidden email]>
Sent: Wednesday, January 1, 2020 8:16 PM
To: [hidden email] <[hidden email]>
Subject: Expansion Microscopy

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*****

Good afternoon.

I am a student at the University of South Carolina School of Medicine working on my Master's thesis; the topic is expansion microscopy (specifically, I'm using the Protein Retention Expansion Microscopy protocol.)  I want to reach out and see if anyone has previously worked with this technique with cardiac tissue, specifically looking at the connexin proteins in intercalated discs?  I have seen references to the MAP technique being used with heart tissue, but not ProExM.​ If so, what has been your experiences (successes and failures)?  Also, does anyone have a good protocol for MAP so that I may be able to look at the differences?  Thank you for any help you can give me.

~ Ashley Ferri
Graduate Research Assistant
University of South Carolina School of Medicine
[hidden email]