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Hi Aleem,
The LOCI bioformats reader for ImageJ should be able to load .sdt files as a stack. You can then use the plot z axis profile tool to output either sum or average curves.
I have found that the LOCI reader doesn't always load the older .sdt files and those involving kinetic measurements. If you have trouble contact me off list and I can send you an alternative (though poorer performing) plugin.
You can also use my phasor plugin available at
http://research.stowers.org/imagejplugins (Plugins>FLIM Tools>histogram 2D phasor jru v1) to select pixels with similar lifetimes and export their summed lifetime profile. I find this to be a powerful complement to pixel-at-a-time analysis.
Jay
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Vladimir Ghukasyan
Sent: Monday, June 24, 2013 12:41 PM
To:
[hidden email]
Subject: Re: Exporting lifetime curves from SPCImage
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Hi Aleem,
You can output the data from the .sdt files (rather than SPCImage files), by using the file data description. Each .sdt file has a header which provides you information about the data structure and location of the curves per pixel. You can create a MatLab script that would allow you to import this and analyze.
Please see TCSPC Handbook on the Becker & Hickl Website ( www.becker-hickl.com / handbookphp.htm) page 604.
Best,
Vladimir
Confocal and Multiphoton Imaging Core
Neuroscience Center
University of North Carolina
On Mon, Jun 24, 2013 at 1:32 PM, Aleem Syed <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear All,
>
> Is there any way I could export lifetime curves from SPCImage, not
> just the lifetime values for all pixels at once to a file? I know how
> to export one curve from one pixel at a time though.
>
> Thank you for your help.
>
> Aleem
> --
> *Best Regards,
> *
> *Aleem Syed
> *
> *Graduate Assistant
> *
> *Emily Smith Research Group
> *
> *0712 Gillman Hall
> *
> *Department of Chemistry
> *
> *Iowa State University
> *
> *Ames Laboratory
> *
> *IA, USA
> *
>
> * *
>
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