FCS + two photon?

[okolab]

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***COMMERCIAL RESPONSE FROM OKOLAB***

 

Okolab offers both microscope enclosure chambers and stage top incubators.

Both of them allow to mix up to three different gas, for instance N2-O2-CO2.

Oxygen in particular can be decreased down to 0.5%.

Of course you can also feed just N2 and CO2 and therefore have 0% O2.

 

This is one of the several unique features of our microscope incubators.

 

Please do not hesitate to contact me off-list for any further info.

 

Best regards,

Luca

 

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Lanzaro Luca, Eng.
Sales and Marketing Manager

 

Okolab s.r.l.
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----- Original Message -----

From: [hidden email]

To: [hidden email]

Sent: Wednesday, December 12, 2007 3:59 AM

Subject: Re: Microscope enclosure chambers

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Farid,

      To the best of my knowledge most of these chambers can control CO2 and temperature but cannot control oxygen concentrations.
However a chamber which controls temperature, O2 and CO2 is available from PeCon, Germany (No commercial interest)
In this chamber oxygen can be decreased down to about 1%. Creating low oxygen conditions is a very challenging task.
I am myself curious to know whether any other commercial microscopy chambers for oxygen control are available.
Good luck for your experiments.

Best,
Venkat

Farid Jalali wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Germaine to the topic of this thread, does anyone have experience maintaining the atmosphere within these microscope-enclosing chambers at hypoxic O2 concentrations (0.5-0%O2)? We are a cancer research laboratory; many solid tumours have hypoxic regions, and we would like to image live cells under hypoxic conditions. The responses we are looking at are very sensitive to O2 and I am trying to get a sense of whether an enclosure or stage -top approach would be best. We are poised to test a Pathology Devices stage top incubator but I am curious to know if anyone has tried something similar with an enclosure.

Cheers
Farid

On Dec 11, 2007 8:00 PM, Stephen Cody <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Prabhakar,

May I suggest you contact Allan Anderson ( Ex. Bio-Rad Engineer) at C lear State Solutions  [hidden email]. He has just launched a new microscope enclosure after consulting many of the scientists around Australia. It has some great features, and it is very competitively priced.

Cheers Steve

Stephen H. Cody

Microscopy Manager

C entral R esource for A dvanced M icroscopy
Ludwig Institute for Cancer Research

PO Box 2008 Royal Melbourne Hospital

Victoria,      3050

Australia

Tel: [phone]    Fax: [phone]

email: [hidden email]

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-----Original Message-----
From: Confocal Microscopy List [[hidden email]] On Behalf Of Andrew Resnick
Sent: Tuesday, 11 December 2007 12:18 AM
To: [hidden email]
Subject: Re: Microscope enclosure chambers

Search the CONFOCAL archive at

http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Ours was made by Solent Scientific, for an upright Leica DM 6000.

It's designed specifically for what you are talking about (which is

also, coincidentally, the type of studies we perform).

Andy

At 04:59 PM 12/6/2007, you wrote:

>Search the CONFOCAL archive at

>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

>

>Hello,

>         We are looking for a microscope enclosure chambers that

> will control temperature, humidity and gas with feedback for long term studies.

>I am looking for enclosures that will also allow ports for tubing to

>come out of the chamber. The tubing is connected to a syringe pump

>outside the enclosure.

>

>Any suggestions with feedback on companies to checkout will be great.

>

>Thanks,

>

>-Prabhakar

Andrew Resnick, Ph. D.

Instructor

Department of Physiology and Biophysics

Case Western Reserve University

216-368-6899 (V)

216-368-4223 (F)

---

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The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research.

--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
Princess Margaret Hospital
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
416-581-7754 STTARR at MaRS Building
416-581-7791 STTARR Microscopy Suite