FEP tubing vs BABB
Locked
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi All, Does anyone know if FEP can stand up to BABB? The obvious test is to put some tubing in BABB, but the corrosion could be slow enough that I can't see it, and I don't want to contaminate the objective (lightsheet application (and I do know this will be an optical nightmare)). Thanks. -- Best, Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310 |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Gary, haven't tried it myself, because, what's the point? FEP has RI close to that of water, so it does not introduce severe aberration when used for sample embedding with water-based immersion media. In BABB a FEP tube would cause bad aberrations (depending on tube dimensions and the NA of your objective lens)... But my guess is it is chemically compatible. FEP is similar to PTFE in many respects, see here: https://www.chemours.com/Teflon_Industrial/en_US/tech_info/techinfo_compare. html Best, zdenek -- Zdenek Svindrych, Ph.D. Research Associate - Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth email: [hidden email] ---------- Původní e-mail ---------- Od: Gary Laevsky <[hidden email]> Komu: [hidden email] Datum: 25. 4. 2018 10:04:07 Předmět: FEP tubing vs BABB "***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi All, Does anyone know if FEP can stand up to BABB? The obvious test is to put some tubing in BABB, but the corrosion could be slow enough that I can't see it, and I don't want to contaminate the objective (lightsheet application (and I do know this will be an optical nightmare)). Thanks. -- Best, Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310 " |
|
|
In reply to this post by Gary Laevsky
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Gary, I don't think it matters whether it is or isn't. FEP tubing has the same RI as water. If you put BABB inside (RI >1.5) you'll have too much spherical aberration to see anything clearly. Just re-hydrate the sample into water and then place it in a microscope-friendly aqueous clearing solution (RIMs, CUBIC-R, PROTOS, Optiview, SeeDB2, etc.) -Doug On Wed, Apr 25, 2018 at 10:00 AM, Gary Laevsky <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi All, > > Does anyone know if FEP can stand up to BABB? The obvious test is to put > some tubing in BABB, but the corrosion could be slow enough that I can't > see it, and I don't want to contaminate the objective (lightsheet > application (and I do know this will be an optical nightmare)). > > Thanks. > > -- > Best, > > Gary Laevsky, Ph.D. > Director, Confocal Imaging Facility > Nikon Center of Excellence > Dept. of Molecular Biology > Washington Rd. > Princeton University > Princeton, New Jersey, 08544-1014 > (O) 609 258 5432 > (C) 508 507 1310 > |
|
|
In reply to this post by Gary Laevsky
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Gary, The R.I. will be the issue more than the FEP tubing holding up against BABB. If you need something to hold your BABB cleared sample then you should be looking for some kind of boro-silicate glass tubing as this will match up with the BABB a lot better (still not exact but better than FEP would). I know Zeiss were talking up boro-silicate for use on their Z.1 as a way of sealing things like 3DISCO away from the sample chamber/objective. I did track some down to a company in Germany who would sell various diameters and custom size the length for you. Cheers Mark _______________________________________ Mark Scott Senior Microscopist Institute of Molecular Bioscience Level 6 North, 306 Carmody Road (Building 80) The University of Queensland Brisbane, Queensland, 4072, Australia [hidden email] On 26/4/18, 12:01 am, "Confocal Microscopy List on behalf of Gary Laevsky" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi All, Does anyone know if FEP can stand up to BABB? The obvious test is to put some tubing in BABB, but the corrosion could be slow enough that I can't see it, and I don't want to contaminate the objective (lightsheet application (and I do know this will be an optical nightmare)). Thanks. -- Best, Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310 |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |