FISH and plant chromosomes

[Cromey, Douglas W - (dcromey)]

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Listers,

Not exactly a confocal question...

Last week I had a visiting scholar referred to me for advice about doing FISH on chromosome spreads from plant cells. This was very much out of my area of expertise. I have put him in contact with a few people at our university, but I was wondering if there are some experts out there who might be able to suggest some reading material or offer up a protocol for him to review?

Thanks in advance!
Doug

------------------------------------------------------------------------------------------
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  LSN 463              email: [hidden email]<mailto:[hidden email]>
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[Hanna_SN]

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Dear Doug,

Please refer to Jenkins G and Hasterok R "BAC 'landing' on chromosomes of
Brachypodium distachyon for comparative genome alignment" Nature Protocols
volume 2, pages 88–98 (2007). The protocol worked for me perfectly well.
Otherwise, I would suggest your friend contacting prof. Robert Hasterok at
the Department of Plant Anatomy and Cytology, University of Silesia,
Poland( [hidden email]) - he has a great expertise in FISH on
plants.

Best wishes,
Hanna

wt., 30 paź 2018 o 00:08 Cromey, Douglas W - (dcromey) <
[hidden email]> napisał(a):

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Listers,
>
> Not exactly a confocal question...
>
> Last week I had a visiting scholar referred to me for advice about doing
> FISH on chromosome spreads from plant cells. This was very much out of my
> area of expertise. I have put him in contact with a few people at our
> university, but I was wondering if there are some experts out there who
> might be able to suggest some reading material or offer up a protocol for
> him to review?
>
> Thanks in advance!
> Doug
>
>
> ------------------------------------------------------------------------------------------
> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>
> office:  LSN 463              email: [hidden email]<mailto:
> [hidden email]>
> voice:  520-626-2824       fax:  [phone]
>
> http://microscopy.arizona.edu/learn/microscopy-imaging-resources-www
> Home of: "Microscopy and Imaging Resources on the WWW"
>
> UA Microscopy Alliance -  http://microscopy.arizona.edu<
> http://microscopy.arizona.edu/>
>

--
Hanna Sas-Nowosielska

Laboratory of Imaging Tissue Structure and Function
Nencki Institute of Experimental Biology
Warsaw, Poland