FISH for miRNA in plants

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Luisa Cortes Luisa Cortes
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FISH for miRNA in plants

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Dear all,

I have been contacted by a researcher that would like to detect microRNAs in plant tissues by FISH.
Since this was very much out of my area of expertise I was wondering if someone might be able to suggest what is the best way to perform these experiments, namely, the best dyes and share protocols and pitfalls.

Thank you so much in advance.

Luisa Cortes

Head of Microscopy Facility
Center for Neuroscience and Cell Biology
University of Coimbra
Portugal