FPs for MP

[Graham Wright-2]

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Dear all,

I'm keen to find out what your favoured pair of fluorescent proteins for
multiphoton microcopy, in which you’re limited to a single excitation
wavelength (to avoid compromising too much with each). And which excitation
wavelengths you use with them.

Thanks in advance,
Graham

—
Graham Wright, PhD
Director, A*STAR Microscopy Platform

T: [phone]
E: [hidden email]
W: www.a-star.edu.sg/imb/Tech-Platforms/AMP-Light-Microscopy

[Ekaterina PAPUSHEVA-2]

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BFP/GFP and BFP/YFP worked well with 800-820 nm excitation in Zebrafish.

Good luck!
Ekaterina

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Graham Wright
Sent: Tuesday, October 16, 2018 3:38 PM
To: [hidden email]
Subject: FPs for MP

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Dear all,

I'm keen to find out what your favoured pair of fluorescent proteins for
multiphoton microcopy, in which you’re limited to a single excitation
wavelength (to avoid compromising too much with each). And which excitation
wavelengths you use with them.

Thanks in advance,
Graham

—
Graham Wright, PhD
Director, A*STAR Microscopy Platform

T: [phone]
E: [hidden email]
W: www.a-star.edu.sg/imb/Tech-Platforms/AMP-Light-Microscopy

[Gabriel KRENS-2]

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To add: Where you can also combine TagBFP + eGFP/Venus + Tetra-methyl Rhodamine (TMR), in case you are able to add labelled dextrans for instance, to excite 3 dyes with 830-840nm

Best
Gabriel


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ekaterina PAPUSHEVA
Sent: Tuesday, October 16, 2018 15:43
To: [hidden email]
Subject: Re: FPs for MP

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Post images on http://www.imgur.com and include the link in your posting.
*****

BFP/GFP and BFP/YFP worked well with 800-820 nm excitation in Zebrafish.

Good luck!
Ekaterina

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Graham Wright
Sent: Tuesday, October 16, 2018 3:38 PM
To: [hidden email]
Subject: FPs for MP

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all,

I'm keen to find out what your favoured pair of fluorescent proteins for multiphoton microcopy, in which you’re limited to a single excitation wavelength (to avoid compromising too much with each). And which excitation wavelengths you use with them.

Thanks in advance,
Graham

—
Graham Wright, PhD
Director, A*STAR Microscopy Platform

T: [phone]
E: [hidden email]
W: www.a-star.edu.sg/imb/Tech-Platforms/AMP-Light-Microscopy