FRAP Fluorophores that photobleach easily

[Jeremy Adler-4]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****


In the context of FRAP,
I would appreciate suggestions and literature references about fluorophores that are easy to photobleach.

Jeremy Adler
BioVis
Uppsala U










När du har kontakt med oss på Uppsala universitet med e-post så innebär det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/

E-mailing Uppsala University means that we will process your personal data. For more information on how this is performed, please read here: http://www.uu.se/en/about-uu/data-protection-policy

[Michał Majkowski-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Once I found NBD bleaching very quickly. The fluorophore was used to label
PE.
best
Michal Majkowski

czw., 15 kwi 2021 o 12:31 Jeremy Adler <[hidden email]> napisał(a):

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> In the context of FRAP,
> I would appreciate suggestions and literature references about
> fluorophores that are easy to photobleach.
>
> Jeremy Adler
> BioVis
> Uppsala U
>
>
>
>
>
>
>
>
>
>
> När du har kontakt med oss på Uppsala universitet med e-post så innebär
> det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör
> det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/
>
> E-mailing Uppsala University means that we will process your personal
> data. For more information on how this is performed, please read here:
> http://www.uu.se/en/about-uu/data-protection-policy
>

[George McNamara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jeremy,

PE, aka R-PE aka Phycoerythrin.

George

On 4/15/2021 6:29 AM, Jeremy Adler wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> In the context of FRAP,
> I would appreciate suggestions and literature references about fluorophores that are easy to photobleach.
>
> Jeremy Adler
> BioVis
> Uppsala U
>
>
>
>
>
>
>

[Tim Feinstein]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jeremy, I had good experience with tdTomato.  It has decent brightness, it bleaches well, red light causes less phototoxicity at photon fluxes needed for bleaching, and the red wavelength lets you use a green fluorophore/FP as a counterstain.  I published a relevant experiment in Nature Chem Bio (2011) 5:278.  It's one of the supplemental figs.  

Best,


T

Timothy Feinstein, Ph.D.


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Jeremy Adler
Sent: Thursday, April 15, 2021 6:30 AM
To: [hidden email]
Subject: FRAP Fluorophores that photobleach easily

*****
To join, leave or search the confocal microscopy listserv, go to:
https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=04%7C01%7Ctnf8%40PITT.EDU%7Cf24408bf497646fd702508d8fff9affb%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637540795176436538%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=01j9MawMZckgqqflxTRHEfPjOev04HXtliUWJWePoLQ%3D&amp;reserved=0
Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&amp;data=04%7C01%7Ctnf8%40PITT.EDU%7Cf24408bf497646fd702508d8fff9affb%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637540795176446533%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=tGx8wovKEeXkVa0b%2Fk4mXPdubHx9%2BBUZLaRzUkXxqio%3D&amp;reserved=0 and include the link in your posting.
*****


In the context of FRAP,
I would appreciate suggestions and literature references about fluorophores that are easy to photobleach.

Jeremy Adler
BioVis
Uppsala U










När du har kontakt med oss på Uppsala universitet med e-post så innebär det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör det kan du läsa här: https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.uu.se%2Fom-uu%2Fdataskydd-personuppgifter%2F&amp;data=04%7C01%7Ctnf8%40PITT.EDU%7Cf24408bf497646fd702508d8fff9affb%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637540795176446533%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=EZYgAsBj5h7FeX56bBIhJxpJKZqrCbwyWoLUl1kBrkA%3D&amp;reserved=0

E-mailing Uppsala University means that we will process your personal data. For more information on how this is performed, please read here: https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.uu.se%2Fen%2Fabout-uu%2Fdata-protection-policy&amp;data=04%7C01%7Ctnf8%40PITT.EDU%7Cf24408bf497646fd702508d8fff9affb%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637540795176446533%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=LDUG72VuwSDf2KtncdWeyVzmM6aNQJYlMJ8jM4tFGNk%3D&amp;reserved=0

[Glyn Nelson]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jeremy,

I like AcGFP- it bleaches quite easily, especially compared to other green FPs.  Old school wtGFP does too, but it's far dimmer.

Glyn