FRAP analysis

[Rickman, Colin]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi

I'm looking for software to analyse FRAP experiments - both intensity measurement and the subsequent model based curve fitting. Obviously the intensity part is relatively simple in ImageJ/FIJI. Doe anybody have a plugin for the subsequent analysis? I've been using the frap_analysis Matlab program for measurement/fitting but the fitting is based on a 2D system.

Any help gratefully received.

Regards

Colin

Dr Colin Rickman
Life Science Interface
Department of Chemistry (WP 2.03)
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)

http://www.lifescienceinterface.hw.ac.uk
http://www.eps.hw.ac.uk/departments/chemistry/cr.htm



--
Heriot-Watt University is a Scottish charity
registered under charity number SC000278.

[simon walker (BI)-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

ImageJ does curve fitting (though I'm not sure if it's suitable for what you have in mind):
Analyze>Tools>Curve Fitting
Paste your values over the values shown, chose your selected model and away you go..

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Colin Rickman
Sent: 14 September 2011 13:34
To: [hidden email]
Subject: FRAP analysis

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi

I'm looking for software to analyse FRAP experiments - both intensity measurement and the subsequent model based curve fitting. Obviously the intensity part is relatively simple in ImageJ/FIJI. Doe anybody have a plugin for the subsequent analysis? I've been using the frap_analysis Matlab program for measurement/fitting but the fitting is based on a 2D system.

Any help gratefully received.

Regards

Colin

Dr Colin Rickman
Life Science Interface
Department of Chemistry (WP 2.03)
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)

http://www.lifescienceinterface.hw.ac.uk
http://www.eps.hw.ac.uk/departments/chemistry/cr.htm



--
Heriot-Watt University is a Scottish charity
registered under charity number SC000278.

[Kristine Willis]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

My group uses GraphPad Prism for curve fitting FRAP data.

Kristine Willis, Ph.D.
Assistant Research Professor
Department of Biology, Reiss 406
Georgetown University
37th & O Sts. NW
Washington, D.C., 20057
202-687-5513

[Arne Seitz]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Colin,

maybe you want to have a look at the software described in the following article:
http://bioinformatics.oxfordjournals.org/content/22/21/2709.long

Cheers Arne

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Colin
> Rickman
> Sent: mercredi 14 septembre 2011 14:34
> To: [hidden email]
> Subject: FRAP analysis
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I'm looking for software to analyse FRAP experiments - both intensity
> measurement and the subsequent model based curve fitting. Obviously the
> intensity part is relatively simple in ImageJ/FIJI. Doe anybody have a plugin
> for the subsequent analysis? I've been using the frap_analysis Matlab
> program for measurement/fitting but the fitting is based on a 2D system.
>
> Any help gratefully received.
>
> Regards
>
> Colin
>
> Dr Colin Rickman
> Life Science Interface
> Department of Chemistry (WP 2.03)
> School of Engineering and Physical Sciences Heriot-Watt University Edinburgh
> EH14 4AS
>
> Tel: [phone] (Office)
>
> http://www.lifescienceinterface.hw.ac.uk
> http://www.eps.hw.ac.uk/departments/chemistry/cr.htm
>
>
>
> --
> Heriot-Watt University is a Scottish charity registered under charity number
> SC000278.