Contact Dr. Steven Vogel, NIH and they give cerulean and Venus based linker. They use aminoacid to link the Cerulean and Venus or they may suggest you some alternative.
Steven S. Vogel [
[hidden email]]
Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:
[hidden email]
http//:www.kcci.virginia.edu
************************
Workshop on FRET Microscopy, March 9-13, 2010
http://www.kcci.virginia.edu/workshop/workshop2010/index.php*************************
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Christoph Wotzlaw
Sent: Thursday, May 14, 2009 7:41 AM
To:
[hidden email]
Subject: FRET: ECFP---EYFP construct
Dear colleagues,
for calibration/control experiments I would like to use a ECFP-short linker-EYFP construct (no real protein in-between). Can someone provide me with it kindly?
Best wishes
Chris
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