FRET/FLIM

[sunandod]

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Dear All
  I am a new member in this list. I would like to do FRET/FLIM between two
proteins for which I have observed in  vitro binding.

Would you direct me towards the first reading(s) which will help me in chalking
out a work flow including microscopy and software.

I have 4 years of experience in Confocal microscopy on fixed cells.

Thank you in advance.

Have a good Sunday.

Sunando

[Periasamy, Ammasi (ap3t)]

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Hi Sunando
There are number of papers available, you can visit our web site to see it or you can Google it.
I also edited a book on FLIM microscopy and this book is focused for both beginners and experts. You can visit amazon.com to see the book prize.
If you wanted to get hands on training you can join our FRET workshop and you can get hands on training on FRET-FLIM techniques. This workshop is focused for beginners, morning theory and afternoon hands on training on various FRET microscopy techniques with live cells or you can bring your own cells to test it. Of course you can get a free FLIM microscopy book too as a part of the workshop tuition fee.
Visit the web site to register and there is no fee for registration. The workshop is during March 11-16, 2013. http://www.kcci.virginia.edu/workshop/workshop2013/index.php 
Hope this helps.

Dr. Ammasi Periasamy
Professor & Center Director
W.M. Keck Center for Cellular Imaging (KCCI)
(A University Imaging Center)
Biology, University of Virginia
Mail or FedEx: 485 McCormick Rd.
Charlottesville, VA 22904.
Office Location: Physical Life Sciences Building (B005)
90, Geldard Drive, Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:[phone]; Email:[hidden email]
http://www.kcci.virginia.edu/contact/peri.php
************************
12th Annual Workshop on FRET Microscopy, March 11-16, 2013
http://www.kcci.virginia.edu/workshop/workshop2013/index.php
*************************

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sunando Datta
Sent: Saturday, February 09, 2013 1:09 PM
To: [hidden email]
Subject: FRET/FLIM

*****
To join, leave or search the confocal microscopy listserv, go to:
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Dear All
  I am a new member in this list. I would like to do FRET/FLIM between two proteins for which I have observed in  vitro binding.

Would you direct me towards the first reading(s) which will help me in chalking out a work flow including microscopy and software.

I have 4 years of experience in Confocal microscopy on fixed cells.

Thank you in advance.

Have a good Sunday.

Sunando

[sunandod]

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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Dear Prof. Periasamy
   Thank you very much for your reply. It is too close for me to attend
this year's workshop. I would try in the next year.
Thank you. I will have a look at your book.
Best regards
Sunando
On 02/10/2013 04:34 AM, Periasamy, Ammasi (ap3t) wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Sunando
> There are number of papers available, you can visit our web site to see it or you can Google it.
> I also edited a book on FLIM microscopy and this book is focused for both beginners and experts. You can visit amazon.com to see the book prize.
> If you wanted to get hands on training you can join our FRET workshop and you can get hands on training on FRET-FLIM techniques. This workshop is focused for beginners, morning theory and afternoon hands on training on various FRET microscopy techniques with live cells or you can bring your own cells to test it. Of course you can get a free FLIM microscopy book too as a part of the workshop tuition fee.
> Visit the web site to register and there is no fee for registration. The workshop is during March 11-16, 2013. http://www.kcci.virginia.edu/workshop/workshop2013/index.php
> Hope this helps.
>
> Dr. Ammasi Periasamy
> Professor & Center Director
> W.M. Keck Center for Cellular Imaging (KCCI)
> (A University Imaging Center)
> Biology, University of Virginia
> Mail or FedEx: 485 McCormick Rd.
> Charlottesville, VA 22904.
> Office Location: Physical Life Sciences Building (B005)
> 90, Geldard Drive, Charlottesville, VA 22904
> Voice: 434-243-7602 (Office); 982-4869 (lab)
> Fax:[phone]; Email:[hidden email]
> http://www.kcci.virginia.edu/contact/peri.php
> ************************
> 12th Annual Workshop on FRET Microscopy, March 11-16, 2013
> http://www.kcci.virginia.edu/workshop/workshop2013/index.php
> *************************
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sunando Datta
> Sent: Saturday, February 09, 2013 1:09 PM
> To: [hidden email]
> Subject: FRET/FLIM
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All
>    I am a new member in this list. I would like to do FRET/FLIM between two proteins for which I have observed in  vitro binding.
>
> Would you direct me towards the first reading(s) which will help me in chalking out a work flow including microscopy and software.
>
> I have 4 years of experience in Confocal microscopy on fixed cells.
>
> Thank you in advance.
>
> Have a good Sunday.
>
> Sunando

--
Dr. Sunando Datta
Assistant Professor
Department of Biological Sciences
Indian Institute of Science Education and Research Bhopal
ITI Gas Rahat Building
Govindpura Industrial Area
Bhopal-462023
Madhya Pradesh
India

[John Oreopoulos]

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To join, leave or search the confocal microscopy listserv, go to:
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*****

I would also mention another excellent book on this topic that was published about the same time, and which did a very good job of summarizing the state of the art (no commercial interest):
 
http://store.elsevier.com/FRET-and-FLIM-Techniques/isbn-9780080549583

Cheers,

John Oreopoulos
Staff Scientist
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca

On 2013-02-10, at 4:20 AM, sunando <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Prof. Periasamy
>  Thank you very much for your reply. It is too close for me to attend this year's workshop. I would try in the next year.
> Thank you. I will have a look at your book.
> Best regards
> Sunando
> On 02/10/2013 04:34 AM, Periasamy, Ammasi (ap3t) wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi Sunando
>> There are number of papers available, you can visit our web site to see it or you can Google it.
>> I also edited a book on FLIM microscopy and this book is focused for both beginners and experts. You can visit amazon.com to see the book prize.
>> If you wanted to get hands on training you can join our FRET workshop and you can get hands on training on FRET-FLIM techniques. This workshop is focused for beginners, morning theory and afternoon hands on training on various FRET microscopy techniques with live cells or you can bring your own cells to test it. Of course you can get a free FLIM microscopy book too as a part of the workshop tuition fee.
>> Visit the web site to register and there is no fee for registration. The workshop is during March 11-16, 2013. http://www.kcci.virginia.edu/workshop/workshop2013/index.php
>> Hope this helps.
>>
>> Dr. Ammasi Periasamy
>> Professor & Center Director
>> W.M. Keck Center for Cellular Imaging (KCCI)
>> (A University Imaging Center)
>> Biology, University of Virginia
>> Mail or FedEx: 485 McCormick Rd.
>> Charlottesville, VA 22904.
>> Office Location: Physical Life Sciences Building (B005)
>> 90, Geldard Drive, Charlottesville, VA 22904
>> Voice: 434-243-7602 (Office); 982-4869 (lab)
>> Fax:[phone]; Email:[hidden email]
>> http://www.kcci.virginia.edu/contact/peri.php
>> ************************
>> 12th Annual Workshop on FRET Microscopy, March 11-16, 2013
>> http://www.kcci.virginia.edu/workshop/workshop2013/index.php
>> *************************
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sunando Datta
>> Sent: Saturday, February 09, 2013 1:09 PM
>> To: [hidden email]
>> Subject: FRET/FLIM
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear All
>>   I am a new member in this list. I would like to do FRET/FLIM between two proteins for which I have observed in  vitro binding.
>>
>> Would you direct me towards the first reading(s) which will help me in chalking out a work flow including microscopy and software.
>>
>> I have 4 years of experience in Confocal microscopy on fixed cells.
>>
>> Thank you in advance.
>>
>> Have a good Sunday.
>>
>> Sunando
>
> --
> Dr. Sunando Datta
> Assistant Professor
> Department of Biological Sciences
> Indian Institute of Science Education and Research Bhopal
> ITI Gas Rahat Building
> Govindpura Industrial Area
> Bhopal-462023
> Madhya Pradesh
> India