FV 300 to multiphoton

[Yongquan Jiang]

Is there any group successfully upgrade an Olympus FV 300 confocal system
to a non-descanned two-photon imaging system? I have a hard time to have
the
detector work in full range.

Thanks for any input.

[Guy Cox-2]

I don't really understand your question.  What do you mean by 'work in
full range'?  Vignetting?  Prof Min Gu at Swinburne University has done
this conversion, I think, and you could contact him directly.  Nuno
Moreno, Jose Feijo and I fitted a transmitted NDD to an old Bio-Rad MRC
1024 with no real problems Nuno Moreno, Jose Feijo & Guy Cox, 2004.
Implementation and evaluation of a detector for forward propagated
second harmonic signals.  Micron, 35, 721-724.  The key thing to avoid
vignetting is that the NDD must be at, or conjugate with, a back focal
plane.

                                     Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Yongquan Jiang
Sent: Saturday, 3 July 2010 7:03 AM
To: [hidden email]
Subject: FV 300 to multiphoton

Is there any group successfully upgrade an Olympus FV 300 confocal
system
to a non-descanned two-photon imaging system? I have a hard time to have

the
detector work in full range.

Thanks for any input.

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04:35:00

[Johannes Helm]

Good afternoon,

we've done it twice here in Oslo.

Ref.:

G. Nase, P.J. Helm, O.P.Ottersen (2005),
A multiphoton laser scanning microscope setup for transcranial in vivo
brain imaging on mice,
Review of Scientific Instruments 76:123702-1-123702-5


and


P. J. Helm, T. Reppen, P. Heggelund (2009),
A setup for combined multi photon laser scanning microscopic and multi
electrode patch clamp experiments on brain slices,
Proc. SPIE 7183:71832Q-1 - 71832Q-8

Both setups are fully operational and in use.

Best wishes,

Johannes



> Is there any group successfully upgrade an Olympus FV 300 confocal system
> to a non-descanned two-photon imaging system? I have a hard time to have
> the
> detector work in full range.
>
> Thanks for any input.
>


--
P. Johannes Helm, M.Sc. PhD
Seniorengineer
CMBN
University of Oslo
Institute of Basic Medical Science
Department of Anatomy
Postboks 1105 - Blindern
NO-0317 Oslo

Voice: [phone]
Fax: [phone]

WWW: folk.uio.no/jhelm