FW: 2P standard

[Richard Cole]

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I agree w/Michael that the Chroma slides are not the best for MP.  I typically use an H&E stained pathology slide.  This seems to be stable >3 years

Rich

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
 
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Dept. of Biomedical Sciences
School of Public Health State University of New York Albany, New York

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Fluorescent plastic slides are not stabile.  Depending on their exposure to light they bleach or change color.  Using a pulsed laser is a good way to make little burn or other chemical reaction spots in them.  Here are two old web pages with some of the problems (and benefits!) explained:  
http://www.einstein.yu.edu/aif/instructions/fluor-ref-slides/01.htm
http://www.einstein.yu.edu/aif/instructions/zeiss/liveduo/Zaxis_bleachingtest/index.htm
_______________________________________
Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

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From: Confocal Microscopy List [[hidden email]] On Behalf Of Mark Cannell [[hidden email]]
Sent: Friday, January 14, 2011 7:39 PM
To: [hidden email]
Subject: Re: 2P standard

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How about Fluorescent plastic slides -I got some free from Chroma.

Cheers


On 15/01/2011, at 12:34 PM, Boswell, Carl A - (cboswell) wrote:

> Hi all,
> I'm looking for some trustworthy, permanent standard(s) to use in a MP
> system to monitor the condition of detectors and the system in
> general.   The goal is a fluorescent source that is immutable.  This
> could done either by having unrestricted replacement of bleached label
> with unbleached label (i.e. a solution), or a uniform and very large
> solid, so that it is unlikely that any one tiny volume will be
> imaged repeatedly.   We've tried several iterations of an aqueous
> solution of label, but sealing the prep does not prevent eventual
> evaporation.  Plus, there seems to be some modification of FITC, for
> example,  in solution over time (a month) regardless of exposure to
> light.  I'm not sure I can trust the consistency of colored plastic
> slides, since they are not manufactured with this role in mind.  One
> possibility is uranyl glass slides, but Corning says they don't make
> these any more.  Does anyone know a source?  We're also considering
> quantum dots in something like immersion oil , in a sealed chamber but
> I don't have any experience with these labels in a hydrophobic
> environment.
>
> Thanks for your insights.
> C
>
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> Univ. of Arizona
> 520-954-7053
> FAX [phone]
>

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[Steffen Dietzel]

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Rich,

what exactly are you looking at then, eosin fluorescence?

Steffen

On 15.01.2011 21:38, Rich Cole wrote:
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>
> I agree w/Michael that the Chroma slides are not the best for MP.  I typically use an H&E stained pathology slide.  This seems to be stable>3 years
>
> Rich
>

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