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We routinely do 5 and even six colors, (see spectrums, sorry rejected by
listserv, list below) with our Leica SP5 in spectral mode.  It has been my
experience that the immunology is more challenging than the imaging.  Just
my 2 ยข.
 
CyQuant
Nissel
Alexa 569, 594 & 633
 
 
Rich
 
 
 
Richard Cole
Research Scientist V
Director: Advanced Light Microscopy Core Unit
Wadsworth Center
 
Research Assistant Professor 
Dept. of Biomedical Sciences 
School of Public Health State University of New York 
P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax 
 
Email  <mailto:
[hidden email]> 
[hidden email]
Website  <
http://www.wadsworth.org/cores/alm/index.htm>
www.wadsworth.org/cores/alm/index.htm
 
 
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