FW: GFP and mCherry

[Sylvie Le Guyader-2]

Med vänlig hälsning / Best regards
 
Sylvie
 
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Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
[phone]

-----Original Message-----
From: Sylvie Le Guyader [mailto:[hidden email]]
Sent: 07 April 2009 16:10
To: '[hidden email]'
Subject: RE: GFP and mCherry

I'd say that antibodies against DsRed and RFP are much more likely to cross
react than GFP and mCherry since DsRed, RFP and mCherry all come from the
same molecule from coral and are very similar whereas GFP comes from a very
different molecule originally from Jelly fish.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
[phone]

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of
> [hidden email]
> Sent: 07 April 2009 14:56
> To: [hidden email]
> Subject: Re: GFP and mCherry
>
> Along those same lines what about anti-DsRed cross reacting with RFP?
>
> Thanks.
>
>
> ------Original Message------
> From: Valeria Berno
> Sender: Confocal Microscopy List
> To: [hidden email]
> ReplyTo: Confocal Microscopy List
> Sent: Apr 7, 2009 8:19 AM
> Subject: GFP and mCherry
>
> Quick question:
>
> Does someone knows if the antibody anti-GFP would cross-react also with
> the mCherry?
>
> I assume the answer is no but i cannot find any specification. I could
> only found no cross-reactivity of DsRed antibodies with GFP......
>
> Thanks in advance
>
> Valeria