FW: Small multi-color fluorescent beads (was "big fluorescent beads")

[Keith Morris]

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Hi John,

By multi-colour, I have used particles of this size from Duke Scientific,
but they were available in red, blue or green fluorescence [each ~50nm
particle in the mixture would be one colour or the other].

I got my 0.044um Green fluorescence microspheres from 'Duke Scientific' [cat
no. G40] many years ago:
http://www.distrilabparticles.com/filters/pdf/Bul120'A'%20FluorPoly.pdf

Duke Scientific's [www.dukescientific.com] website seem to be related
somehow to Thermoscientific these days, as their web address now leads
there:
http://www.thermoscientific.com/ecomm/servlet/productscatalog?categoryId=874
36&&storeId=11152

i.e. my G40 0.04um GF particles:
http://www.thermoscientific.com/ecomm/servlet/productsdetail?productId=11963
237&groupType=PRODUCT&searchType=0&storeId=11152&from=search
and they still seem to do the 50nm red, green or blue fluorescence
particles.

Perhaps it might be worth asking Thermoscientific as a starting point if you
want dual fluorescence within each 50nm particle [or try Polysciences for a
custom special], unless someone comes up with a better suggestion.

Regards

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford  OX3 7BN,
United Kingdom.

Telephone:  [phone]
Email:  [hidden email]
Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of John Oreopoulos
Sent: 29 March 2012 15:03
To: [hidden email]
Subject: Small multi-color fluorescent beads (was "big fluorescent beads")

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Somewhat related to this last posting, can anyone recommend a source of
small (50 nm diameter or less) multi-color (at least two distinct emission
spectra) fluorescent microspheres? Commercial vendors may also contact me
directly offline.  

Thank you.

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2012-03-28, at 4:59 AM, Keith Morris wrote:

> *****
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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> As David has said, do check out Polysciences:
>
> http://www.polysciences.com/Catalog/40/categoryId__4
>
> They used to manufacture beads to your specification if required, and I
> don't think that has changed or that it was that expensive - but it's been
> many years since I last did it.
>
> Regards
>
> Keith
>
>

---------------------------------------------------------------------------

> Dr Keith J. Morris,
> Molecular Cytogenetics and Microscopy Core,
> Laboratory 00/069 and 00/070,
> The Wellcome Trust Centre for Human Genetics,
> Roosevelt Drive,
> Oxford  OX3 7BN,
> United Kingdom.
>
> Telephone:  [phone]
> Email:  [hidden email]
> Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]

On

> Behalf Of David Knecht
> Sent: 27 March 2012 19:10
> To: [hidden email]
> Subject: Re: big fluorescent beads
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Polysciences has fluorescent polystyrene in that size range:
> http://www.polysciences.com/Catalog/Department/81/categoryId__338/
>
> Kisker (Discover science) has 10micron fluorescent silica and 20 micron
> biotinylated that should be easy to label with fluorescent avidin.  
> http://www.microparticles-nanospheres.com/micro_silicia.htm
>
> Bang has 10-25 micron fluorescent
> http://www.bangslabs.com/products/fluorescent_microspheres
>
>
> On Mar 27, 2012, at 6:25 PM, Tobias Baskin wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi all,
>> Does anyone know of a commerical source of fluorescent beads
>> that are around 25 microns in diameter? We are sorting large cells
>> and need something in that size range to calibrate the instrument.
>>
>> Many thanks,
>>   Tobias
>> --
>>      _      ____          __   ____
>>     /  \   /          / \    /   \ \       Tobias I. Baskin
>>    /   /  /          /   \   \      \        Professor
>>   /_ /   __      /__ \   \       \__    Biology Department
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>> /      /          /         \   \       \      University of
>> Massachusetts
>> /      / ___   /           \   \__/  \ ____ Amherst, MA, 01003
>> www.bio.umass.edu/biology/baskin
>> Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
>
> Visiting Professor David Knecht
> Beatson Institute for Cancer Research
> University of Glasgow
> Switchback Road, Bearsden
> Glasgow Scotland G61 1BD
> UK
>
>
>
>
>
>
>
> Visiting Professor David Knecht
> Beatson Institute for Cancer Research
> University of Glasgow
> Switchback Road, Bearsden
> Glasgow Scotland G61 1BD
> UK