Filtering for K-2 IND

[Joe Summers]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,

I'm using a Nikon microscope for use with an older Technical Instruments K-2 IND confocal spinning disk attachment.

I've posted a picture of our setup at:  http://www.mtholyoke.edu/~jsummers/Nikon_K2IND_confocal.jpg

For our purposes, we'd like to be able to filter light coming from the specimen before it enters the CCD in the trinocular.
Most filter slots I'm aware of sit between the trinocular and the nosepiece, however, since our spinning disk unit does not have a filter slot, that isn't an option for us.

I'm writing to ask if anyone has any suggestions for how to integrate a filter (e.g. a BG-39 shortpass) into this system.  Does any company make a filter adapter that could fit into the nosepiece, or trinoc, or c-mount CCD?  It would be nice to find a ready-made piece rather than having to drill holes in our observation tube, or machine parts.  Any input would be appreciated!

Thanks and best,
Joe Summers

------------------------------------
Postdoc, Physics Dept.
Optoelectronics Group
Mt. Holyoke College
ph: [phone]
http://www.mtholyoke.edu/~jsummers/

[Craig Brideau]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We have a Nikon E800 with a filter bank integrated into the trinocular.  It looks a bit like yours but is a bit taller.  I have no idea if it would fit your particular scope though; check with Nikon.

Craig

On Fri, May 16, 2008 at 11:49 AM, Joe Summers <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,

I'm using a Nikon microscope for use with an older Technical Instruments K-2 IND confocal spinning disk attachment.

I've posted a picture of our setup at:  http://www.mtholyoke.edu/~jsummers/Nikon_K2IND_confocal.jpg

For our purposes, we'd like to be able to filter light coming from the specimen before it enters the CCD in the trinocular.
Most filter slots I'm aware of sit between the trinocular and the nosepiece, however, since our spinning disk unit does not have a filter slot, that isn't an option for us.

I'm writing to ask if anyone has any suggestions for how to integrate a filter (e.g. a BG-39 shortpass) into this system.  Does any company make a filter adapter that could fit into the nosepiece, or trinoc, or c-mount CCD?  It would be nice to find a ready-made piece rather than having to drill holes in our observation tube, or machine parts.  Any input would be appreciated!

Thanks and best,
Joe Summers

------------------------------------
Postdoc, Physics Dept.
Optoelectronics Group
Mt. Holyoke College
ph: [phone]
http://www.mtholyoke.edu/~jsummers/

[Joe Summers]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Thanks, all (esp. Mel Brenner at Nikon) for your suggestions.

The space between the trinoc head and the confocal unit was large enough to accommodate a filter and seems to work nicely:

http://www.mtholyoke.edu/~jsummers/Nikon_K2IND_filter.jpg

Best -Joe

On May 16, 2008, at 2:51 PM, Craig Brideau wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We have a Nikon E800 with a filter bank integrated into the trinocular.  It looks a bit like yours but is a bit taller.  I have no idea if it would fit your particular scope though; check with Nikon.

Craig

On Fri, May 16, 2008 at 11:49 AM, Joe Summers <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,

I'm using a Nikon microscope for use with an older Technical Instruments K-2 IND confocal spinning disk attachment.

I've posted a picture of our setup at:  http://www.mtholyoke.edu/~jsummers/Nikon_K2IND_confocal.jpg

For our purposes, we'd like to be able to filter light coming from the specimen before it enters the CCD in the trinocular.
Most filter slots I'm aware of sit between the trinocular and the nosepiece, however, since our spinning disk unit does not have a filter slot, that isn't an option for us.

I'm writing to ask if anyone has any suggestions for how to integrate a filter (e.g. a BG-39 shortpass) into this system.  Does any company make a filter adapter that could fit into the nosepiece, or trinoc, or c-mount CCD?  It would be nice to find a ready-made piece rather than having to drill holes in our observation tube, or machine parts.  Any input would be appreciated!

Thanks and best,
Joe Summers

------------------------------------
Postdoc, Physics Dept.
Optoelectronics Group
Mt. Holyoke College
ph: [phone]
http://www.mtholyoke.edu/~jsummers/

------------------------------------

Postdoc, Physics Dept.

Optoelectronics Group

Mt. Holyoke College

ph: [phone]

http://www.mtholyoke.edu/~jsummers/