First Course Announcement-Optical Microscopy, Woods Hole, MA

[Hard, Robert]

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*This is the FIRST announcement for the OMIBS course, Marine Biology
Laboratory, Woods Hole, MA:*



*Title:* Optical Microscopy and Imaging in the Biomedical Sciences



*History:* Established in 1978 and offered annually since then.



*When:* September 27 - October 7, 2012



*Application Deadline: *Applications accepted through June 22, 2012



*Course Directors: *

Robert Hard, University at Buffalo                   Colin S. Izzard,
University at Albany

Phone: [716] 829-3521                                    Phone: [518]
439-7121

Email: [hidden email]                        E mail:
[hidden email]



*Course Description**:*



*Where:* In the *newly renovated *and *modernized facilities *in the Loeb
Bldg at the Marine Biology Laboratory, Woods Hole, MA

* *

*What:* *Imaging fundamentals* to *state of the art,* *multi-dimensional* *wide
field, fluorescence, confocal, multiphoton, multispectral, deconvolution,
and super-resolution microscopic systems.*

* *

*How: *Lectures, *hands on exercises, problem sets, demonstrations* and *
discussions,* *specifically designed to drive home the principles and
applications* of modern microscopic methods. Students will learn to *obtain
and interpret microscope images* of high quality, to perform *quantitative
optical measurements*, and to *produce digital record*s for documentation
and analysis. In the problem sets, *small groups of students will acquire,
analyze, interpret and present imaging data, thereby demonstrating their
progress toward mastering microscopy and imaging skills.*



*Equipment:* Microscopes, Cameras, and Software from *19 World Renowned
Companies*.* ***

* *

*For Whom:*

Designed primarily for research scientists, postdoctoral trainees and
advanced graduate students in animal, plant, medical and material sciences.
Facility Directors and non-biologists seeking a comprehensive introduction
to microscopy and digital imaging in the biomedical sciences will benefit
greatly from this course. There are no specific prerequisites, but an
understanding of the basic principles of optics and imaging is desirable.
The course is limited to 26 students.



*Specimens: *Live and fixed specimens will be provided for use in
laboratory exercises and demonstrations. *However, students are encouraged
to bring their own biological (cell cultures, prepared slides, etc. ) and
material specimens to use in exercises throughout the course, where
appropriate. Cell culture facilities are available for student use.
Students also are encouraged to make appointments with our experienced
Commercial Faculty outside of scheduled classes to acquire data from their
specimens using the state of the art equipment available. Students are
highly encouraged to discuss their individual research problems with both
the academic and commercial faculty.*



*Topics To Be Covered Include:*



Principles of microscope design and image formation, image resolution, and
image contrast.



Bright field, dark field, phase contrast, polarized light, differential
interference contrast, interference reflection, and fluorescence microscopy



Cameras, digital imaging, signal to noise ratio, recording, enhancement,
processing and analysis



Advanced fluorescent techniques (eg. FRAP, FLIP, speckle imaging, FRET,
FLIM, fluorescence polarization, photoactivation, photoswitching,
fluorescence correlation spectroscopy, multi-spectral imaging, filter
cubes, fluorescent dyes/proteins)



Confocal scanning microscopy (LSM and spinning disc), multiphoton
excitation fluorescence microscopy and structured illumination systems



Image deconvolution, digital image restoration



3D image acquisition, display, and analysis



Live cell imaging



Super-resolution techniques (STED, PALM, STORM, SIM)





*Tuition:* $2925 (Includes room and board, text, handouts, supplies)



*Admission application and information:*

Carol Hamel, Admissions Coordinator

Marine Biological Laboratory

7 MBL Street

Woods Hole, MA 02543-1015

[phone]

Internet: [hidden email]

WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)





*Recent faculty have included:*



Frank Bright, *University at Buffalo*

Richard Day, *Indiana University School of Medicine*

Joseph A DePasquale, *Morphogenyx*

Paul Goodwin, *Applied Precision, Inc.*

Brian Herman, *U. Texas Health Science Center*

Shinya Inoue, *Marine Biology Laboratory*

Frederick Lanni, *Carnegie Mellon University*

Jennifer Lippincott-Schwartz, *Cell Biology and Metabolism, NIH*

James McNally, *National Cancer Institute, NIH*

Butch Moomaw, *Hamamatsu Corp.*

John M. Murray,* Indiana University*

Rudolph Oldenberg, *Marine Biology Laboratory*

Michael Shribak, *Marine Biology Laboratory***

David Piston, *Vanderbilt University*

Michael Shribak, *Marine Biology Laboratory***

Wade Sigurdson, *University at Buffalo*

Clare M. Waterman, *Cell Biology and Physiology Center, NIH*





*See Also:*

http://www.mbl.edu/education/courses/special_topics/om.html