First overlay image

[Jeremy Adler-4]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Out of curiosity and wanting to give the  correct citation, does anyone know when and where the first overlay image was published ?
Overlays typically put different images into separate layers of an RGB image to show areas of overlap.

My guess is the early 1990s, possibly limited by the charges made for colour printing, so references to the use of overlays, even if not printed would also be interesting.

[phil laissue-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

If multiple wavelengths acquired separately and presented in the same frame
would count as overlay, this goes at least back to the first use of
dichroics:
Ploem, J. S.:Die Möglichkeit der Auflichtfluoreszenzmethoden bei
Untersuchungen von Zellen in Durchströmungskammern und Leightonröhren. Xth
Symposium d. Gesellschaft f. Histochemie, 1965. Acta Histochem. Suppl. 7,
339–343, 1967.
Ploem, J. S.: The use of a vertical illuminator with interchangeable
dichroic mirrors for fluorescence microscopy with incident light. Zeitschr.
f. wiss. Mikroskopie 68, 129–142, 1967.

However, micrographs were acquired on colour film, but it's not clear if
that was double exposure on the same frame.

_________________________________________
Philippe Laissue, PhD, Director of Bioimaging Unit
School of Biological Sciences, Room 4.17
University of Essex, Colchester CO4 3SQ, UK
(0044) 01206 872246 / (0044) 07842 676 456
[hidden email]
privatewww.essex.ac.uk/~plaissue


On 25 June 2014 08:30, Jeremy Adler <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Out of curiosity and wanting to give the  correct citation, does anyone
> know when and where the first overlay image was published ?
> Overlays typically put different images into separate layers of an RGB
> image to show areas of overlap.
>
> My guess is the early 1990s, possibly limited by the charges made for
> colour printing, so references to the use of overlays, even if not printed
> would also be interesting.
>

[mmodel]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Some beautiful overlay images from hundred years ago:

http://www.loc.gov/exhibits/empire/gorskii.html

Mike
________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Jeremy Adler <[hidden email]>
Sent: Wednesday, June 25, 2014 3:30 AM
To: [hidden email]
Subject: First overlay image

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Out of curiosity and wanting to give the  correct citation, does anyone know when and where the first overlay image was published ?
Overlays typically put different images into separate layers of an RGB image to show areas of overlap.

My guess is the early 1990s, possibly limited by the charges made for colour printing, so references to the use of overlays, even if not printed would also be interesting.

[Keith Morris]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jeremy

Fox-Talbot and Louis Daguerre  created the first proper photographs of
images down a microscope* when they invented the B&W photographic process
back in the 1840s, so it seems likely that the first one to create coloured
photo overlays was an earlier pioneer in colour photography (the Autochrome
colour photography process was introduced in 1907 and there were earlier
colour processes). Certainly in 1854 John Snow created a coloured overlay
map showing the location of Cholera victims deaths overlaid onto a street
map of Soho in London  which enabled him to correctly  identify the source
of the epidemic (a water pump in Broad Street).  Coloured overlays were
regularly created manually using tracing paper (invented in 1809) or sheet
glass, which may or may not have been photographed in colour.

Microscopes that can overlay images from two different samples (one head,
two microscopes), i.e. the bridge or comparison microscope, was regularly
used with photography, e.g. in 1929 Goddard's comparison microscope was
developed for forensic ballistics in order to compare two bullets thought to
have been fired from the same gun (and modern colour camera variants are
still used for this today). Back in the 1969 the Quantimet 720 image
analyser was introduced that overlaid a single orange coloured binary
thresholded image over that of a digitised B&W  image captured from a
microscope, following on from pioneering work in the 1960s regarding
digitising and quantifying  images from the microscope, although typically
the images analysed were B&W (as indeed are our fluorescence images today).
Given that Disney created triple B&W silver grain film masters of his
original colour Snow White film, one each for the red, green and blue
channel, back in the 1930s, seems likely that those 1960s digital pioneers
also experimented with RGB colour overlays.

So the first multi-colour overlay was no doubt created a while ago, the
exact date probably lost in history and also depending on how you define
'colour overlay' (normal fluorescence microscope R-G-B layering typically
only works with up to three B&W channels, so you could easily go beyond that
today with say up to 8000 layers in Photoshop CS6).

Interesting question.

Regards, Keith

*In fact it is likely that Thomas Wedgewood (son the Josiah the potter and a
relation of Charles) took the first B&W photo of an image down a microscope
around 1799, as the Solar Microscope, an magnifying variant of the Camera
Obscura, was also available to him, but sadly Thomas couldn't find a way to
fix the photographic image and so make them relatively immune to effects of
light. Some of Thomas's unfixed photos did survive at least to the 1890s as
his B&W photographs didn't fade if kept in total darkness. Thomas's
pioneering photographic work was published by his friend Humphrey Davy in
1802.

-----------------------------------------------------------
Dr Keith J Morris
Cellular Imaging Microscopy Core,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford,
OX3 7BN,
United Kingdom.
 
Tel:  +44   ( 0 ) 1865  287568
Email:   [hidden email]
Webpage: www.well.ox.ac.uk/microscopy-facilities
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Jeremy Adler
Sent: 25 June 2014 08:31
To: [hidden email]
Subject: First overlay image

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Out of curiosity and wanting to give the  correct citation, does anyone know
when and where the first overlay image was published ?
Overlays typically put different images into separate layers of an RGB image
to show areas of overlap.

My guess is the early 1990s, possibly limited by the charges made for colour
printing, so references to the use of overlays, even if not printed would
also be interesting.

[Rosey Van Driel]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dr Richard GW Anderson (University of Texas Southwestern at Dallas) published two colour fluorescence images in the mid - late 1980's. We double exposed slide film with images collected from FITC and Texas Red. Just changed filter cube and re-exposed the same piece of colour slide film. Where the image became orange/yellowish was the overlapping signal. I don't think it was the first lab to do this.
But it was fun! Look in the journal "Cell" around that time.

Best regards,
Rosey

Rosey van Driel
Manager, Transmission Electron Microscopy (TEM)
Institute for Frontier Materials, GTP Research


Deakin University
Geelong Waurn Ponds Campus, Locked Bag 20000, Geelong, VIC 3220
[phone]
Mobile [phone]
[hidden email]
www.deakin.edu.au
https://www.deakin.edu.au/research/facilities/electron-microscope/index.php
Deakin University CRICOS Provider Code 00113B

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Keith Morris
Sent: Wednesday, 25 June 2014 9:37 PM
To: [hidden email]
Subject: Re: First overlay image

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jeremy

Fox-Talbot and Louis Daguerre  created the first proper photographs of images down a microscope* when they invented the B&W photographic process back in the 1840s, so it seems likely that the first one to create coloured photo overlays was an earlier pioneer in colour photography (the Autochrome colour photography process was introduced in 1907 and there were earlier colour processes). Certainly in 1854 John Snow created a coloured overlay map showing the location of Cholera victims deaths overlaid onto a street map of Soho in London  which enabled him to correctly  identify the source of the epidemic (a water pump in Broad Street).  Coloured overlays were regularly created manually using tracing paper (invented in 1809) or sheet glass, which may or may not have been photographed in colour.

Microscopes that can overlay images from two different samples (one head, two microscopes), i.e. the bridge or comparison microscope, was regularly used with photography, e.g. in 1929 Goddard's comparison microscope was developed for forensic ballistics in order to compare two bullets thought to have been fired from the same gun (and modern colour camera variants are still used for this today). Back in the 1969 the Quantimet 720 image analyser was introduced that overlaid a single orange coloured binary thresholded image over that of a digitised B&W  image captured from a microscope, following on from pioneering work in the 1960s regarding digitising and quantifying  images from the microscope, although typically the images analysed were B&W (as indeed are our fluorescence images today).
Given that Disney created triple B&W silver grain film masters of his original colour Snow White film, one each for the red, green and blue channel, back in the 1930s, seems likely that those 1960s digital pioneers also experimented with RGB colour overlays.

So the first multi-colour overlay was no doubt created a while ago, the exact date probably lost in history and also depending on how you define 'colour overlay' (normal fluorescence microscope R-G-B layering typically only works with up to three B&W channels, so you could easily go beyond that today with say up to 8000 layers in Photoshop CS6).

Interesting question.

Regards, Keith

*In fact it is likely that Thomas Wedgewood (son the Josiah the potter and a relation of Charles) took the first B&W photo of an image down a microscope around 1799, as the Solar Microscope, an magnifying variant of the Camera Obscura, was also available to him, but sadly Thomas couldn't find a way to fix the photographic image and so make them relatively immune to effects of light. Some of Thomas's unfixed photos did survive at least to the 1890s as his B&W photographs didn't fade if kept in total darkness. Thomas's pioneering photographic work was published by his friend Humphrey Davy in 1802.

-----------------------------------------------------------
Dr Keith J Morris
Cellular Imaging Microscopy Core,
The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford,
OX3 7BN,
United Kingdom.

Tel:  +44   ( 0 ) 1865  287568
Email:   [hidden email]
Webpage: www.well.ox.ac.uk/microscopy-facilities


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jeremy Adler
Sent: 25 June 2014 08:31
To: [hidden email]
Subject: First overlay image

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Out of curiosity and wanting to give the  correct citation, does anyone know when and where the first overlay image was published ?
Overlays typically put different images into separate layers of an RGB image to show areas of overlap.

My guess is the early 1990s, possibly limited by the charges made for colour printing, so references to the use of overlays, even if not printed would also be interesting.

Important Notice: The contents of this email are intended solely for the named addressee and are confidential; any unauthorised use, reproduction or storage of the contents is expressly prohibited. If you have received this email in error, please delete it and any attachments immediately and advise the sender by return email or telephone.

Deakin University does not warrant that this email and any attachments are error or virus free.