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Paula Sampaio |
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Hello all,
I have a collaborator that need to stain microtubules in multiple structures of coenocyte, syncytium and cellularizing endosperm of early cereals grains. Do someone could give us some tips about a good method to fix and stain this kind of tissue sections for visualization of microtubules in confocal microscopy? Thank you very much. Best regards, Paula *********************************************************
Paula Sampaio, PhD.
IBMC - Instituto de Biologia Molecular e Celular
Universidade do Porto
Rua Campo Alegre 823
P4150-180 PORTO
PORTUGAL
Tel: +351 226074900 Ext: 1606
Fax: +351 226099157
email: [hidden email]
Web page:
http://www.ibmc.up.pt/
*********************************************************
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Tobias Baskin |
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Hi,
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello all, ********************************************************* Paula Sampaio, PhD. IBMC - Instituto de Biologia Molecular e Celular Universidade do Porto Rua Campo Alegre 823 P4150-180 PORTO PORTUGAL Tel: +351 226074900 Ext: 1606 Fax: +351 226099157 email: [hidden email] Web page: http://www.ibmc.up.pt/ ********************************************************* --
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\ Tobias I.
Baskin
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Department
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\ University of
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http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 -
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Stanislav Vitha |
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In reply to this post by Paula Sampaio
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi, also look at earlier papers by F. Baluska, he has been using Brown's method with Steedman's wax embedding for immunofluorescence localization of tubulin, actin and a large number of other proteins in plants (mostly in roots, but should work for kernels, too). Baluska, F., Parker, J.S. and Barlow, P.W. (1992) Specific patterns of cortical and endoplasmic microtubules associated with cell growth and tissue differentiation in roots of maize (Zea mays L.). J. CellSci. 103: 191-200. If you decide to use embedding in Steedman's wax, I have a detailed protocol on our web site - the fixation is for actin, but the embedding procedure is the same: http://microscopy.tamu.edu/lab-protocols/Steedman_wax.pdf Stan On Fri, 31 Aug 2007 13:24:58 -0400, Tobias Baskin <[hidden email]> wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > >Hi, > Check papers by R.C. Brown and B.E. Lemmon. THese two have >published quite a few papers on this. Their protocol (s) would be a >great place to start. > > Tobias > >>Search the CONFOCAL archive at >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello all, >> >>I have a collaborator that need to stain microtubules in multiple >>structures of coenocyte, syncytium and cellularizing endosperm of >>early cereals grains. Do someone could give us some tips about a >>good method to fix and stain this kind of tissue sections for >>visualization of microtubules in confocal microscopy? >> >>Thank you very much. >> >>Best regards, >> >>Paula >> >> >> >> >>********************************************************* >>Paula Sampaio, PhD. >>IBMC - Instituto de Biologia Molecular e Celular >>Universidade do Porto >>Rua Campo Alegre 823 >>P4150-180 PORTO >>PORTUGAL >> >>Tel: +351 226074900 Ext: 1606 >>Fax: +351 226099157 >> >>email: [hidden email] >> >>Web page: http://www.ibmc.up.pt/ >>********************************************************* > > >-- > _ ____ __ ____ > / \ / / \ / \ \ Tobias I. Baskin > / / / / \ \ \ Biology Department > /_ / __ /__ \ \ \__ 611 N. Pleasant St. > / / / \ \ \ University of > / / / \ \ \ Amherst, MA, 01003 >/ / ___ / \ \__/ \ ____ >http://www.bio.umass.edu/biology/baskin/ >Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 |
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