Flow cell for flushing samples during imaging
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Edrun Andrea Schnell |
Flow cell for flushing samples during imaging
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi all! I have a question to ask on behalf of a collegue regarding flow cells. I don't know anything about flow cells myself, so I appologize if my questions/requirements are obvious or impossible... We are in search of a flow cell for flushing samples (chitin) during imaging. It should fit a Zeiss Axiovert 100M and/or a Leica TCS SP5 and it should be easily implemented and removed. Do any of you have any suggestions? Best regards, Edrun -- Edrun Andrea Schnell Divisional engineer, Dept. of Physics, NTNU Hogskoleringen 5 7491 Trondheim, Norway Tel.: +47 73 59 36 69 |
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Hugo.Ostermann |
AW: Flow cell for flushing samples during imaging
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Edrun, have a look at the FCS2 Chamber and contact me off list if you need a quotation. http://www.bioptechs.com/Products/FCS2/fcs2.html With kind regards Hugo Ostermann CHROMAPHOR Analysen-Technik GmbH Mühlenkamp 37 D-59387 Ascheberg - Germany Tel: ++49 - 2593-928.600 Fax: ++49 - 2593-928.601 mail: [hidden email] URL: http://www.chromaphor.de Sitz Ascheberg-Westfalen Amtsgericht - Registergericht Coesfeld HRB 7703 Geschäftsführer: Hugo Ostermann -----Ursprüngliche Nachricht----- Von: Confocal Microscopy List [mailto:[hidden email]] Im Auftrag von Edrun Andrea Schnell Gesendet: Freitag, 7. März 2008 09:37 An: [hidden email] Betreff: Flow cell for flushing samples during imaging Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi all! I have a question to ask on behalf of a collegue regarding flow cells. I don't know anything about flow cells myself, so I appologize if my questions/requirements are obvious or impossible... We are in search of a flow cell for flushing samples (chitin) during imaging. It should fit a Zeiss Axiovert 100M and/or a Leica TCS SP5 and it should be easily implemented and removed. Do any of you have any suggestions? Best regards, Edrun -- Edrun Andrea Schnell Divisional engineer, Dept. of Physics, NTNU Hogskoleringen 5 7491 Trondheim, Norway Tel.: +47 73 59 36 69 |
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Deanne Veronica Catmull |
Re: Flow cell for flushing samples during imaging
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In reply to this post by Edrun Andrea Schnell
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Edrun, It all depends on what your requirements are? Do you require an opened or closed chamber? Aerobic or anaerobic conditions? The Bioptechs chamber is very good, but extremely pricey if you don't need the sophisticated set up. You can also make up Flow Cell chambers yourself. There are a variety of papers that could prob give you some details if you search through PubMed. Kind regards, Deanne. Deanne Catmull Research Assistant School of Dental Science University of Melbourne 720 Swanston St Carlton 3010 Fax: 9341-1597 Ph: 9341-1577 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Edrun Andrea Schnell Sent: Friday, March 07, 2008 7:37 PM To: [hidden email] Subject: Flow cell for flushing samples during imaging Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi all! I have a question to ask on behalf of a collegue regarding flow cells. I don't know anything about flow cells myself, so I appologize if my questions/requirements are obvious or impossible... We are in search of a flow cell for flushing samples (chitin) during imaging. It should fit a Zeiss Axiovert 100M and/or a Leica TCS SP5 and it should be easily implemented and removed. Do any of you have any suggestions? Best regards, Edrun -- Edrun Andrea Schnell Divisional engineer, Dept. of Physics, NTNU Hogskoleringen 5 7491 Trondheim, Norway Tel.: +47 73 59 36 69 |
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Edrun Andrea Schnell |
Re: Flow cell for flushing samples during imaging
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Deanne! Thanks for the reply! We need a cell for which we can have pump or syringe driven flushing - a closed system which allows for constant and controlled flushing. We don't know exactly what requirements we have yet, it would be best to buy something which can be used for different things. We have tried to make some things ourselves, but it wasn't good enough. Regards, Edrun -- Edrun Andrea Schnell Divisional engineer, Dept. of Physics, NTNU Hogskoleringen 5 7491 Trondheim, Norway Tel.: +47 73 59 36 69 On Tue, 11 Mar 2008, Deanne Veronica Catmull wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hi Edrun, > > It all depends on what your requirements are? Do you require an opened > or closed chamber? Aerobic or anaerobic conditions? The Bioptechs > chamber is very good, but extremely pricey if you don't need the > sophisticated set up. You can also make up Flow Cell chambers yourself. > There are a variety of papers that could prob give you some details if > you search through PubMed. > > Kind regards, > Deanne. > > Deanne Catmull > Research Assistant > School of Dental Science > University of Melbourne > 720 Swanston St > Carlton 3010 > Fax: 9341-1597 > Ph: 9341-1577 > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of Edrun Andrea Schnell > Sent: Friday, March 07, 2008 7:37 PM > To: [hidden email] > Subject: Flow cell for flushing samples during imaging > > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hi all! > > I have a question to ask on behalf of a collegue regarding flow cells. I > don't know anything about flow cells myself, so I appologize if my > questions/requirements are obvious or impossible... We are in search of > a > flow cell for flushing samples (chitin) during imaging. It should fit a > Zeiss Axiovert 100M and/or a Leica TCS SP5 and it should be easily > implemented and removed. Do any of you have any suggestions? > > Best regards, > Edrun > > -- > > Edrun Andrea Schnell > Divisional engineer, > Dept. of Physics, NTNU > Hogskoleringen 5 > 7491 Trondheim, Norway > Tel.: +47 73 59 36 69 > |
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Csucs Gabor |
Re: Flow cell for flushing samples during imaging
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Edrun LIS (www.lis.ch) also offers such chambers. I don't know how their prices relate to the Bioptechs ones. No commercial interest! Cheers Gabor -- Gabor Csucs Light Microscopy Centre, ETH Zurich Schafmattstrasse 18, HPM F16 CH-8093, Zurich, Switzerland Web: www.lmc.ethz.ch Phone: +41 44 633 6221 Fax: +41 44 632 1298 e-mail: [hidden email] |
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Barbara Foster |
Re: Flow cell for flushing samples during imaging
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In reply to this post by Edrun Andrea Schnell
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi, Edrun
Take a look at Ibidi also. (No commercial interest). Barbara Foster, President Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com MME is now scheduling customized, on-site courses through July 2008. Call us today for details. At 05:35 AM 3/11/2008, you wrote: Search the CONFOCAL archive at |
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Ian Harper-2 |
Re: Flow cell for flushing samples during imaging
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In reply to this post by Edrun Andrea Schnell
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Edrun If you are looking for a quality pump that has neither the inherent limitations of the peristaltic pumps nor the limited capacity of the syringe type systems, then you should look at the amazing epicycloidal pump made (and patented) by a local (Australian) engineer - I have no commercial interest, and have owned one such pump which we use sporadically (not many people do flow here) for some years.. it has flushing (hi and low flow) capabilities, and gives superb smooth (that's my technical term) continuous low flow. I don;t have the contact details at hand, but pls contact me and i will look it up. regards Ian. Edrun Andrea Schnell wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hi Deanne! > > Thanks for the reply! We need a cell for which we can have pump or syringe > driven flushing - a closed system which allows for constant and controlled > flushing. We don't know exactly what requirements we have yet, it would > be best to buy something which can be used for different things. We have > tried to make some things ourselves, but it wasn't good enough. > > Regards, > Edrun > > > -- > > Edrun Andrea Schnell > Divisional engineer, > Dept. of Physics, NTNU > Hogskoleringen 5 > 7491 Trondheim, Norway > Tel.: +47 73 59 36 69 > > On Tue, 11 Mar 2008, Deanne Veronica Catmull wrote: > > >> Search the CONFOCAL archive at >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >> Hi Edrun, >> >> It all depends on what your requirements are? Do you require an opened >> or closed chamber? Aerobic or anaerobic conditions? The Bioptechs >> chamber is very good, but extremely pricey if you don't need the >> sophisticated set up. You can also make up Flow Cell chambers yourself. >> There are a variety of papers that could prob give you some details if >> you search through PubMed. >> >> Kind regards, >> Deanne. >> >> Deanne Catmull >> Research Assistant >> School of Dental Science >> University of Melbourne >> 720 Swanston St >> Carlton 3010 >> Fax: 9341-1597 >> Ph: 9341-1577 >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] On >> Behalf Of Edrun Andrea Schnell >> Sent: Friday, March 07, 2008 7:37 PM >> To: [hidden email] >> Subject: Flow cell for flushing samples during imaging >> >> Search the CONFOCAL archive at >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >> Hi all! >> >> I have a question to ask on behalf of a collegue regarding flow cells. I >> don't know anything about flow cells myself, so I appologize if my >> questions/requirements are obvious or impossible... We are in search of >> a >> flow cell for flushing samples (chitin) during imaging. It should fit a >> Zeiss Axiovert 100M and/or a Leica TCS SP5 and it should be easily >> implemented and removed. Do any of you have any suggestions? >> >> Best regards, >> Edrun >> >> -- >> >> Edrun Andrea Schnell >> Divisional engineer, >> Dept. of Physics, NTNU >> Hogskoleringen 5 >> 7491 Trondheim, Norway >> Tel.: +47 73 59 36 69 >> >> -- >< >< >< >< >< >< >< >< >< >< >< >< >< Dr Ian S. Harper, Director Monash Micro Imaging School of Biomedical Sciences Building 13C, Monash University Clayton, VIC 3800, Australia Email: [hidden email] Tel: +61 3 9905 5635; Fax: +61 3 9905 2733 Mobile: 0408 314168 General enquiries and info: http://microimaging.monash.org >< >< >< >< >< >< >< >< >< >< >< >< >< |
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