Fluorescence in oil
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hey all, I have an application question (not necessarily confocal related). Need to add a fluorescent dye/probe to a mineral oil. Any and all suggestions much appreciated. Thanks, Rod ______________________________________________ Rodney Kwok, Ph.D. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Laurdan, prodan, or nile red? They do ok in non polar environments I believe. Others may have better suggestions. Craig On Jan 27, 2017 5:00 PM, "Rodney Kwok" <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hey all, I have an application question (not necessarily confocal related). Need to add a fluorescent dye/probe to a mineral oil. Any and all suggestions much appreciated. Thanks, Rod ______________________________________________ Rodney Kwok, Ph.D. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** DiI, DiO, DiA, DiR are good candidates as well. John Oreopoulos > On Jan 27, 2017, at 8:18 PM, Craig Brideau <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Laurdan, prodan, or nile red? They do ok in non polar environments I > believe. Others may have better suggestions. > > Craig > > On Jan 27, 2017 5:00 PM, "Rodney Kwok" <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hey all, > > I have an application question (not necessarily confocal related). Need > to add a fluorescent dye/probe to a mineral oil. Any and all suggestions > much appreciated. > > Thanks, > Rod > > ______________________________________________ > > Rodney Kwok, Ph.D. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Awesome, thanks all for the suggestions. Regards, Rod On Fri, Jan 27, 2017 at 7:48 PM John Oreopoulos <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > DiI, DiO, DiA, DiR are good candidates as well. > > John Oreopoulos > > > On Jan 27, 2017, at 8:18 PM, Craig Brideau <[hidden email]> > wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Laurdan, prodan, or nile red? They do ok in non polar environments I > > believe. Others may have better suggestions. > > > > Craig > > > > On Jan 27, 2017 5:00 PM, "Rodney Kwok" <[hidden email]> wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hey all, > > > > I have an application question (not necessarily confocal related). Need > > to add a fluorescent dye/probe to a mineral oil. Any and all suggestions > > much appreciated. > > > > Thanks, > > Rod > > > > ______________________________________________ > > > > Rodney Kwok, Ph.D. > ______________________________________________ Rodney Kwok, Ph.D. 587.889.4978 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** consider using Oil Red O http://www.sigmaaldrich.com/catalog/search?interface=All&term=oil+red+o&lang=en®ion=US&N=0+220003048&focus=product You probably do not need to use ultra-pure stuff, so could save some money by going with not-so-pure products. See also google search: fluorescent dye mineral oil https://www.google.com/webhp?sourceid=chrome-instant&ion=1&espv=2&ie=UTF-8#q=fluorescent+dye+mineral+oil One of the hits sells by the gallon: http://www.riskreactor.com/fluorescent-dye-tracers/auto-car-black-light-dyes/1-gal-oiltracerc8-petroleum-and-mineral-oil-uv-tracer/ and has more sections http://www.riskreactor.com/fluorescent-dye-tracers/ See also the OIL-GLO series at http://www.spectroline.com/fluorescent-dyes-industrial PDf helpfully includes English and metric units: • Several Sizes — Choose from 8 oz (237 ml), pint (473 ml) or quart (946 ml) bottles as well as gallon (3.8 L) containers. Larger sizes also available. If any of these are highly photostable, please let me (and the listserv) know - could make nice microscope performance test reagents (no evaporation?) re: concurrent thread. enjoy, George p.s. I am a big fan of DiI (Sigma-Alrdich's lowest purity grade) for labeling blood vessels (aka "vessel painting"), see https://www.ncbi.nlm.nih.gov/pubmed/?term=24052350 Price wise, probably more expensive than Oil Red O or by-the gallon (or liters) oil tracers. On 1/27/2017 6:02 PM, Rodney Kwok wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hey all, > > I have an application question (not necessarily confocal related). Need > to add a fluorescent dye/probe to a mineral oil. Any and all suggestions > much appreciated. > > Thanks, > Rod > > ______________________________________________ > > Rodney Kwok, Ph.D. -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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