Fluorescence polarization with Leica SP5

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Alessandro Esposito Alessandro Esposito
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Fluorescence polarization with Leica SP5

Dear all,
   does anyone have experience with fluorescence anisotropy imaging with a
Leica SP5? I am interested to solutions for coupling the light source. I am
considering to directly couple supercontinuum source (by Fianium) we have,
but I would need to direct couple it  thorugh the NIR input port with a 20/80
beam splitter on something like that... this, clearly, at detriment of collection
efficiency. Direct coupling would allow also to control the plane of the
polarization by a half-wave plate. I would like to pass through the AOBS, but I
do not think there is a possibility for direct coupling without dismantling the
scan-head...

Cheers,

Alessandro
Laser Analytics Group
Dept Chemical Engineering and Biotechnology
University Cambridge