*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
We are trying a new pre-embed immunoEM protocol using streptavadin-Alexa 546/1.4nm
nanogold conjugate (Nanoprobes) as our tertiary detection reagent. We are using the probe in
fixed Drosophila body wall muscle, and we are getting a lot of signal from the mitochondria,
however our primary antibody very cleanly stains the nuclear lamina. It seems that the
nanogold is the culprit, and we are not sure how to effectively block this cross reaction. We
have used 5% non-fat milk and that has helped, but still leaves substantial background in the
mitochondria. Just wondering if anyone else has come across this problem.
Locked
