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Dear all,
I know the infamous SNR topic has been extensively discussed on this list, but I'd like to know if someone has a good reference (I'd prefer a >1995 article for easy access) on how SNR has a role in detecting changes in fluorescent labeling (immunocytochemistry in my case). For exemple, if I detect a 50% change in labeling (from 250 to 125 in 8bit confocal images, with a background of 5), what are the chances I can detect a similar 50% change, but with a 10X fainter labeling (that is, a drop from 25 to 12.5 with the same background) ? Is the SNR 125/5=25 in the first case, and 2.5 in the second case ?
Christophe
Christophe Leterrier
Postdoc
INSERM UMR641 Neurobiology of ionic channels
IFR Jean Roche - Mediterranee University
Marseille, France
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